Siderophore coated magnetic iron nanoparticles: Rational designing of water soluble nanobiosensor for visualizing Al 3+ in live organism

Raju, Muralikrishna; Srivastava, Sakshi; Nair, Ratish R.; Raval, Ishan H.; Haldar, Soumya; Chatterjee, Pabitra B.

doi:10.1016/j.bios.2017.06.013

Cited by 21 publications

(22 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An insight into the possibility of SPION/PVD formation was also confirmed by the FTIR (Fig. S2 ) which was in agreement with previous reports 27 , 30 , 32 . However, SPION/PVD conjugates were previously used as analytical platforms.…”

Section: Discussionsupporting

confidence: 91%

“…Besides, the bare SPIONs can be degraded via the body metabolism causing an overload of iron ions in tissues. Inspired by reports that used Fe 3 O 4 NPs to selectively adsorb siderophores as a microbial chelator with high affinity 26 , 27 , we demonstrated the capability of magnetic NPs chelated with purified bacterial PVD. Previous studies revealed that SPION can be conjugated with any drugs and/or natural compounds containing sulfhydryl (-SH), amine (-NH 2 ), phosphate (PO 4 3- ), hydroxyl (-OH), and carboxyl (-COOH) groups or their combinations 28 , 29 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Targeted SPION siderophore conjugate loaded with doxorubicin as a theranostic agent for imaging and treatment of colon carcinoma

Nosrati

Abnous

Alibolandi

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Recently, the siderophores have opened new horizons in nanomedicine. The current study aimed to design a theranostic platform based on superparamagnetic iron oxide nanoparticles-pyoverdine (SPION/PVD) conjugates bound to MUC1 aptamer (MUC1Apt) and loaded with doxorubicin (DOX) as an anti-cancer agent. The SPION/PVD complex was covalently conjugated to MUC1Apt and loaded with DOX to prepare a targeted drug delivery system (SPION/PVD/MUC1Apt/DOX). The investigation of cellular cytotoxicity and uptake of formulations by MTT and flow cytometry in both MUC1 positive (C26) and MUC1 negative (CHO) cell lines revealed that MUC1Apt could improve both cellular uptake and toxicity in the C26 cell line. The evaluation of tumor-targeting activity by in vivo bio-distribution showed that the targeted formulation could enhance tumor inhibitory growth effect and survival rate in C26 tumor-bearing mice. Furthermore, the potential of synthesized SPION/PVD/MUC1Apt/DOX complex as diagnostic agents was investigated by magnetic resonance imaging (MRI) which improved the contrast of tumor site in MRI. Our findings confirm that aptamer-targeted PVD chelated the SPION as a diagnostic agent and loaded with DOX as a chemotherapeutic drug, would be beneficial as a novel theranostic platform.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Targeted SPION siderophore conjugate loaded with doxorubicin as a theranostic agent for imaging and treatment of colon carcinoma

Nosrati

Abnous

Alibolandi

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…To assess the performance of H 2 L for bio‐imaging of Zn 2+ in biological milieu, live whole organism Artemia (a marine crustacean) was used (Figure ) instead of the default practice of working in cultured cell lines ( in‐vitro ). Because of their small body size, tiny life span, easy to hatch, and minimum skill needed to nurture has developed the brine shrimp Artemia as a model for studying the increased bio‐accumulation of different heavy metal ions ,,. Control experiments with live marine crustacean exposed to the aqueous solution of H 2 L did not show any bright fluorescence image (control image, Figure a), while blue fluorescence image (Figure b) was observed for the brine shrimps exposed to Zn 2+ .…”

Section: Resultsmentioning

confidence: 99%

Visualizing Zn²⁺ in Living Whole Organism Artemia by a Natural Fluorimetric Intermediate Siderophore

et al. 2017

Self Cite

View full text Add to dashboard Cite

Aeruginic acid (H 2 L), a weakly fluorescent natural ionophore, was synthesized via one-pot mechanochemical grinding at room-temperature. This sulfur containing intermediate siderophore showed unique specificity towards Zn 2 + in 100% water at physiological pH even in presence of other challenging metal ions particularly, paramagnetic metal ions such as Fe 3 + , Co 2 + , Ni 2 + , and Cu 2 + . H 2 L also displayed a naked eye observable instant color change under UV light in presence of Zn 2 + , in aqueous solution and in solid phase as well. The receptor H 2 L with 49 nM limit of detection, upon excitation at 360 nm, reversibly binds one equivalent Zn 2 + as evidenced from Job's plot, ESI-MS, and 1 H NMR spectroscopy. Being fully water soluble, non-toxic, and permeable in nature, this natural ionophore can visualize Zn 2 + in the gastrointestinal tract of live whole brine shrimp Artemia. We are not aware of any report on bio-imaging of Zn 2 + in living organism.

show abstract

“…Siderophores have vast importance in pharmaceutical and agricultural industries (Ahmed and Holmström 2014). For instance, non-toxic nanobiosensor for detection of heavy metal contamination in water (Raju et al 2017), bioimaging of heavy metals in marine animals (Nair et al 2015;Raju et al 2015;Mudhulkar et al 2017), as a fluorosensor for the detection of K + in water (Raju et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization of phenazine-1-carboxylic acid by Klebsiella sp. NP-C49 from the coral environment in Gulf of Kutch, India

et al. 2019

Self Cite

View full text Add to dashboard Cite

Coral-associated microbes from Marine National Park (MNP), Gulf of Kutch (GoK), Gujarat, India, were screened for siderophore production. Maximum siderophore-producing isolate NP-C49 and its compound were identified and characterized. The isolate was identified as Klebsiella sp. through 16S rRNA genes sequencing (GenBank accession nos. KY412519 and MTCC 25160). Antibiotic susceptibility profile against 20 commercial antibiotics showed its more sensitivity compared to human pathogenic strain, i.e., Klebsiella pneumonia. The compound was identified as phenazine-1-carboxylic acid (PCA) using the multinuclear ID ( 1 H and 13 C) and 2D ( 1 H-1 H COSY and 1 H-13 C HETCOR) NMR along with high-resolution mass spectrometry. No significant difference in the bacterial growth in the presence of PCA, FeCl 3 and Fe(OH) 3 indicated involvement of factors other than PCA in bacterial growth. The study first reports the identification and characterization of PCA from Klebsiella sp. both from terrestrial and marine sources. Keywords Coral environment • phenazine-1-carboxylic acid • Klebsiella sp. • Gulf of Kutch Communicated by Erko Stackebrandt. Neha P. Patel and M. Raju contributed equally to the manuscript.

show abstract

Siderophore coated magnetic iron nanoparticles: Rational designing of water soluble nanobiosensor for visualizing Al 3+ in live organism

Cited by 21 publications

References 44 publications

Targeted SPION siderophore conjugate loaded with doxorubicin as a theranostic agent for imaging and treatment of colon carcinoma

Targeted SPION siderophore conjugate loaded with doxorubicin as a theranostic agent for imaging and treatment of colon carcinoma

Visualizing Zn²⁺ in Living Whole Organism Artemia by a Natural Fluorimetric Intermediate Siderophore

Characterization of phenazine-1-carboxylic acid by Klebsiella sp. NP-C49 from the coral environment in Gulf of Kutch, India

Contact Info

Product

Resources

About

Siderophore coated magnetic iron nanoparticles: Rational designing of water soluble nanobiosensor for visualizing Al 3+ in live organism

Cited by 21 publications

References 44 publications

Targeted SPION siderophore conjugate loaded with doxorubicin as a theranostic agent for imaging and treatment of colon carcinoma

Targeted SPION siderophore conjugate loaded with doxorubicin as a theranostic agent for imaging and treatment of colon carcinoma

Visualizing Zn2+ in Living Whole Organism Artemia by a Natural Fluorimetric Intermediate Siderophore

Characterization of phenazine-1-carboxylic acid by Klebsiella sp. NP-C49 from the coral environment in Gulf of Kutch, India

Contact Info

Product

Resources

About

Visualizing Zn²⁺ in Living Whole Organism Artemia by a Natural Fluorimetric Intermediate Siderophore