Side-effects of pesticides on larvae of Chrysoperla carnea (Neuroptera, Chrysopidae): actual state of the laboratory method

Vogt, Heidrun; Degrande, Paulo Eduardo; Just, John J.; Klepka, S.; Kühner, Ch.; Nickless, A.; Ufer, A.; Waldburger, M.; Waltersdorfer, A.; Bigler, F.

doi:10.1007/978-1-4615-5791-3_14

Cited by 17 publications

(17 citation statements)

References 4 publications

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“…H. armigera larvae were reared with a corn flour-based artificial diet (32), while adults were maintained with a 10% honey solution. The diet of green lacewings consisted of Ephestia kuehniella eggs (Koppert Biological Systems, The Netherlands) for larvae and a specific artificial diet for adults (39).…”

Section: Methodsmentioning

confidence: 99%

Lack of Detrimental Effects of Bacillus thuringiensis Cry Toxins on the Insect Predator Chrysoperla carnea : a Toxicological, Histopathological, and Biochemical Analysis

Rodrigo-Simón

Maagd

Avilla

et al. 2006

Appl Environ Microbiol

102

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The effect of Cry proteins of Bacillus thuringiensis on the green lacewing (Chrysoperla carnea) was studied by using a holistic approach which consisted of independent, complementary experimental strategies. Tritrophic experiments were performed, in which lacewing larvae were fed Helicoverpa armigera larvae reared on Cry1Ac, Cry1Ab, or Cry2Ab toxins. In complementary experiments, a predetermined amount of purified Cry1Ac was directly fed to lacewing larvae. In both experiments no effects on prey utilization or fitness parameters were found. Since binding to the midgut is an indispensable step for toxicity of Cry proteins to known target insects, we hypothesized that specific binding of the Cry1A proteins should be found if the proteins were toxic to the green lacewing. In control experiments, Cry1Ac was detected bound to the midgut epithelium of intoxicated H. armigera larvae, and cell damage was observed. However, no binding or histopathological effects of the toxin were found in tissue sections of lacewing larvae. Similarly, Cry1Ab or Cry1Ac bound in a specific manner to brush border membrane vesicles from Spodoptera exigua but not to similar fractions from green lacewing larvae. The in vivo and in vitro binding results strongly suggest that the lacewing larval midgut lacks specific receptors for Cry1Ab or Cry1Ac. These results agree with those obtained in bioassays, and we concluded that the Cry toxins tested, even at concentrations higher than those expected in real-life situations, do not have a detrimental effect on the green lacewing when they are ingested either directly or through the prey.

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Section: Methodsmentioning

confidence: 99%

Lack of Detrimental Effects of Bacillus thuringiensis Cry Toxins on the Insect Predator Chrysoperla carnea : a Toxicological, Histopathological, and Biochemical Analysis

Rodrigo-Simón

Maagd

Avilla

et al. 2006

Appl Environ Microbiol

102

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“…The experiments were carried out using standard methods developed within the framework of the IOBC-WPRS working group pesticides and beneficial organisms (Jacas and Viñuela 1994;Vogt et al 1998Vogt et al , 2000Van de Veire et al 2001). Both rearing and bioassays were done in a controlled environmental cabinet [25 ± 2°C, 75 ± 10% R.H., 16:8 (L:D) photoperiod].…”

Section: Bioassaysmentioning

confidence: 99%

Effects of pesticides commonly used in peach orchards in Brazil on predatory lacewing Chrysoperla carnea under laboratory conditions

et al. 2008

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Effects of the maximum field recommended concentration of five pesticides currently used on peaches in Brazil; abamectin, deltamethrin, methoxyfenozide, phosmet and trichlorfon were tested on the predator Chrysoperla carnea (Stephens) (Neuroptera, Chrysopidae). Dimethoate was used as positive standard. Three analytical laboratory tests, based on IOBC-working group pesticides and beneficial organisms guidelines were used: (1) exposure to fresh pesticide residue on glass plates of (a) larvae (susceptible life stage) and (b) adults (less susceptible life stage); (2) direct spraying of eggs and pupae; (3) exposure of larvae and adults to pesticide residues on plant leaves at different intervals after application (persistence). In tests 1, abamectin was slightly harmful to C. carnea larvae and phosmet and trichlorfon were slightly harmful and moderately harmful to C. carnea adults, respectively. After direct spraying of eggs and pupae, all the pesticides were harmless. In the persistence tests, abamectin and trichlorfon were classified as short lived and therefore they could be considered for use in Integrated Pest Management (IPM) programs under special conditions (i.e. reduced direct contact). Phosmet, however, caused between 56.3 and 75.0% mortality up to 30 days after treatment and it was rated as persistent. No sublethal effects were detected in the reproductive behaviour of adults (fecundity and fertility) compared with the control in any treatment. In conclusion, the insecticides with little or no toxicity to C. carnea such as abamectin, deltamethrin and methoxyfenozide could be considered as IPM-compatible, subject to further field studies.

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“…Procedure of the test The experiments were carried out based on the method defined by IOBC (Bigler, 1988;Vogt et al, 1998). To do the test, two-day-old larvae of the common green lacewing were placed on glass plates sprayed with a potter tower to get 2 mg of wet deposit per cm 2 of each pesticide or tap water (as control) and left to dry.…”

Section: Iobc Approachmentioning

confidence: 99%

Impacts of the pesticides imidacloprid, propargite, and pymetrozine on Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae): IOBC and life table assays

et al. 2006

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The effects of imidacloprid, propargite, and pymetrozine on the common green lacewing, Chrysoperla carnea (Stephens) were investigated in the laboratory, using the IOBC-system and the life table response experiment. Residual glass plate bioassays were carried out using two-day-old larvae at the Iranian maximum field recommended rate of each commonly used pesticide. All three tested pesticides produced significant adverse effects on pre-imaginal survival (p < 0.01). Imidacloprid had no significant effect on fecundity, but propargite and pymetrozin caused significant reductions (p < 0.05). According to the IOBC classification, imidacloprid was found to be harmless (E = 27.44%); propargite (E = 49.78%) and pymetrozine (E = 66.9%) were slightly harmful. Life table assays revealed that imidacloprid and propargite had no significant effects on the intrinsic rate of natural increase, while pymetrozine caused a 34% reduction in r m value (p < 0.05). Propargite was non-toxic to C. carnea under the tested conditions. In the case of imidacloprid, using a glass plate as test substrate led to underestimation of its effects. The life table assay showed more adverse effects of pymetrozine than the IOBC method.

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Side-effects of pesticides on larvae of Chrysoperla carnea (Neuroptera, Chrysopidae): actual state of the laboratory method

Cited by 17 publications

References 4 publications

Lack of Detrimental Effects of Bacillus thuringiensis Cry Toxins on the Insect Predator Chrysoperla carnea : a Toxicological, Histopathological, and Biochemical Analysis

Lack of Detrimental Effects of Bacillus thuringiensis Cry Toxins on the Insect Predator Chrysoperla carnea : a Toxicological, Histopathological, and Biochemical Analysis

Effects of pesticides commonly used in peach orchards in Brazil on predatory lacewing Chrysoperla carnea under laboratory conditions

Impacts of the pesticides imidacloprid, propargite, and pymetrozine on Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae): IOBC and life table assays

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