Side-by-Side Comparison of the Biological Characteristics of Human Umbilical Cord and Adipose Tissue-Derived Mesenchymal Stem Cells

Abstract

Both human adipose tissue-derived mesenchymal stem cells (ASCs) and umbilical cord-derived mesenchymal stem cells (UC-MSCs) have been explored as attractive mesenchymal stem cells (MSCs) sources, but very few parallel comparative studies of these two cell types have been made. We designed a side-by-side comparative study by isolating MSCs from the adipose tissue and umbilical cords from mothers delivering full-term babies and thus compared the various biological aspects of ASCs and UC-MSCs derived from the same individual, in one study. Both types of cells expressed cell surface markers characteristic of MSCs. ASCs and UC-MSCs both could be efficiently induced into adipocytes, osteoblasts, and neuronal phenotypes. While there were no significant differences in their osteogenic differentiation, the adipogenesis of ASCs was more prominent and efficient than UC-MSCs. In the meanwhile, ASCs responded better to neuronal induction methods, exhibiting the higher differentiation rate in a relatively shorter time. In addition, UC-MSCs exhibited a more prominent secretion profile of cytokines than ASCs. These results indicate that although ASCs and UC-MSCs share considerable similarities in their immunological phenotype and pluripotentiality, certain biological differences do exist, which might have different implications for future cell-based therapy.

Useful properties of undifferentiated mesenchymal stromal cells and adipose tissue as the source in liver-regenerative therapy studied in an animal model of severe acute fulminant hepatitis

“…At this point, these cells are not recommended for clinical use. Although some authors [37,43,44] have published that MSCs could transform spontaneously, by following them in longterm in vitro expansion culture, interaction with tumors stroma and genetic manipulation [21]. Rubio et al [43] retracted their results in 2010.…”

Conditioned Media from Human Adipose Tissue-Derived Mesenchymal Stem Cells and Umbilical Cord-Derived Mesenchymal Stem Cells Efficiently Induced the Apoptosis and Differentiation in Human Glioma Cell Lines In Vitro

“…ASCs and UC-MSCs were isolated and amplified as described elsewhere [19]. ASCs and UC-MSCs were analyzed by multichannel flow cytometry using a standard Becton-Dickinson FACS Aria instrument and the CellQuest Pro software (BD Biosciences).…”

Stem cells; mesenchymal stem cells and current clinical applications

“…Kemik ili¤i, adipoz doku, göbek kordonu, kordon kanı, karaci¤er, beyin, di pulpası, deri ve fetal dokularda bulunan farklılamamı, ço¤alma kapasitesi yüksek, kendini farklılamadan yenileyebilme özelli¤ine sahip, mezodermal farklılama yetisi çok fazla olan kök hücrelerdir. [15,16] Mezenkimal kök hüc-reler kemik, kıkırdak, kas gibi farklı hücre tiplerine dönüüm kapasitelerinin yanında ürettikleri birçok büyüme faktörü ve sitokinler nedeni ile immün baskılayıcı ve trofik etki potansiyeline de sahiptir. [15] hücrelerine farklılaabilmeleri gerekmektedir.…”

“…Ya¤ doku ve kemik ili¤i MKH ile benzer fenotip, antiapoptotik özellik ve morfolojileri paylaırlar fakat izolasyon verimlili¤i daha düük oldu¤u için kullanımları kısıtlıdır. [15,16,18] Kemik ili¤i ve ya¤ dokusu kaynaklı MKH'ler benzer morfoloji ve immün fenotiplere sahip olmalarına ra¤men, ya¤ dokusu daha yüksek konsantrasyonda MKH içerir. Fakat kemik ili¤i kaynaklı olan MHK'ler daha uzun süredir çalııldı¤ı için klinik güvenilirli¤i ve uygulamasına ait veriler de daha fazladır.…”