Sickle cell membranes and oxidative damage

Rice‐Evans, Catherine; Omorphos, Savvas C.; Baysal, E.

doi:10.1042/bj2370265

Cited by 144 publications

(82 citation statements)

References 36 publications

(33 reference statements)

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“…The action of DTT in vitro suggests that circulating sickle cells are already deprived of reduced thiols (Kamp et al , 2001). Indeed, sickle cells are reported to contain lower levels of membrane thiols (Rank et al , 1985; Rice‐Evans et al , 1986). HOCl also reacts with membrane thiols.…”

Section: Discussionmentioning

confidence: 99%

“…This is particularly significant in diseases such as SCA in which vascular oxidative stress is increased (Rice‐Evans et al , 1986) with accumulations of highly reactive oxygen species (ROS) including the superoxide anion, hydrogen peroxide and the hydroxyl radical (Sies, 1997; Chirico & Pialoux, 2012; Voskou et al , 2015). Myeloperoxidase released from activated neutrophils may also add to oxidant challenge in SCA, through production of hypochlorous acid (HOCl) from hydrogen peroxide (Vissers et al , 1994; Mutze et al , 2003; Zhang et al , 2013).…”

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confidence: 99%

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Oxidative stress and phosphatidylserine exposure in red cells from patients with sickle cell anaemia

et al. 2018

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SummaryPhosphatidylserine (PS) exposure increases as red cells age, and is an important signal for the removal of senescent cells from the circulation. PS exposure is elevated in red cells from sickle cell anaemia (SCA) patients and is thought to enhance haemolysis and vaso‐occlusion. Although precise conditions leading to its externalisation are unclear, high intracellular Ca2+ has been implicated. Red cells from SCA patients are also exposed to an increased oxidative challenge, and we postulated that this stimulates PS exposure, through increased Ca2+ levels. We tested four different ways of generating oxidative stress: hypoxanthine and xanthine oxidase, phenazine methosulphate, nitrite and tert‐butyl hydroperoxide, together with thiol modification with N‐ethylmaleimide (NEM), dithiothreitol and hypochlorous acid (HOCl), in red cells permeabilised to Ca2+ using bromo‐A23187. Unexpectedly, our findings showed that the four oxidants significantly reduced Ca2+‐induced PS exposure (by 40–60%) with no appreciable effect on Ca2+ affinity. By contrast, NEM markedly increased PS exposure (by about 400%) and slightly but significantly increased the affinity for Ca2+. Dithiothreitol modestly reduced PS exposure (by 25%) and HOCl had no effect. These findings emphasise the importance of thiol modification for PS exposure in sickle cells but suggest that increased oxidant stress alone is not important.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Oxidative stress and phosphatidylserine exposure in red cells from patients with sickle cell anaemia

et al. 2018

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“…[12] Specifically, sickle erythrocytes and their membranes are susceptible to endogenous free radical-mediated oxidative damage that correlates with the proportion of irreversibly sickled erythrocytes. [13] Furthermore, accumulation of hydrogen peroxide (H 2 O 2 ) decreases the half life of erythrocytes by increasing oxidation of polyunsaturated fatty acids of cell membrane [14] and can oxidize haemoglobin to methaemoglobin. [9,15] Methaemoglobin does not bind reversibly with oxygen.…”

Section: Introductionmentioning

confidence: 99%

Levels of two oxidative stress indicators of human sickle erythrocytes incubated in aqueous extracts of Anacardium occidentale, Psidium guajava and Terminalia catappa.

Chikezie

2011

Free Radicals and Antioxidants

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“…Free radical action and lipid peroxidation have been suggested as potentially important causative agents of aging and several types of human diseases [24]. Reactive oxygen species (ROS) have been implicated in damage to erythrocytes in patients with β-thalassemia, sickle cell anemia, glucose-6-phosphate dehydrogenase deficiency and other hemoglobinopathies [25,26]. Aqueous extracts of Conyza canadensis interact in some way with the biological membranes.…”

Section: Resultsmentioning

confidence: 99%

Extract from Conyza canadensis as a modulator of plasma protein oxidation induced by peroxynitrite in vitro

et al. 2010

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Abstract:The antioxidative activity of the extract from Conyza canadensis in plasma treated with peroxynitrite (ONOO -) (0.1 mM) was studied. C. canadensis is known to possess a broad set of pharmacological effects because of content of various antioxidants, antiplatelet and anticoagulant compounds. The aim of our study was to assess if this extract protects plasma proteins against oxidative/nitrative damages induced by ONOO -. The plasma components are continuously exposed to reactive oxygen/nitrogen species action. Peroxynitrite evokes oxidative stress and induces undesirable effects in biological systems and causes damage to biomolecules. The extract from Conyza (50 -2500 mg/ml) caused a dose-dependent reduction of protein nitration by 90%. The oxidation of plasma proteins was diminished by about 75%. ONOO -oxidized the plasma thiol groups and this process was inhibited by tested extract. The level of reduced protein thiols was increased thrice at the lowest concentration of extract (50 mg/ml). The highest concentration of extract decreased twice the level of protein thiols in reduced forms and increased the homocysteine level about 4.5 times. The obtained results demonstrated that the extract from Conyza possesses antioxidative properties in vitro, protects plasma proteins against toxicity induced by peroxynitrite and has modulating effects on thiol/disulfide redox status.

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Sickle cell membranes and oxidative damage

Cited by 144 publications

References 36 publications

Oxidative stress and phosphatidylserine exposure in red cells from patients with sickle cell anaemia

Oxidative stress and phosphatidylserine exposure in red cells from patients with sickle cell anaemia

Levels of two oxidative stress indicators of human sickle erythrocytes incubated in aqueous extracts of Anacardium occidentale, Psidium guajava and Terminalia catappa.

Extract from Conyza canadensis as a modulator of plasma protein oxidation induced by peroxynitrite in vitro

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