SiBaoChongCao exhibited anti-fatigue activities and ameliorated cancer cachexia in mice

Shen, Qiang; Miao, Chunxiao; Zhang, Wanli; Li, Yiwei; Chen, Qiao-Qiao; Li, Xiaoxiang; Li, Xuan

doi:10.1039/c9ra00718k

Cited by 9 publications

(7 citation statements)

References 54 publications

(49 reference statements)

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“…In the second experiment, male BALB/c mice with the same initial body weight were randomly divided into three groups (eight mice per group): healthy group without tumour (healthy group), C26 tumour‐bearing mice group (C26 model group), and C26 tumour‐bearing mice treated with 20 mg/kg DCSD group (C26 + DCSD group). Cancer cachexia in mice was induced as reported in our previous paper 18 . Briefly, on Day 0, mice were implanted subcutaneously in the right flank with 100 μL (1.0 × 10 6 ) C26 tumour cells.…”

Section: Methodsmentioning

confidence: 99%

Carnosol and its analogues attenuate muscle atrophy and fat lipolysis induced by cancer cachexia

Shen

et al. 2021

J cachexia sarcopenia muscle

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Background Cancer cachexia is a multifactorial debilitating syndrome that directly accounts for more than 20% of cancer deaths while there is no effective therapeutic approach for treatment of cancer cachexia. Carnosol (CS) is a bioactive diterpene compound present in Lamiaceae spp., which has been demonstrated to have antioxidant, anti‐inflammatory, and anticancer properties. But its effects on cancer cachexia and the possible mechanism remain a mystery. Methods The in vitro cell models of C2C12 myotube atrophy and 3T3‐L1 mature adipocyte lipolysis were used to check the activities of CS and its synthesized analogues. C26 tumour‐bearing BALB/c mice were applied as the animal model to examine their therapeutic effects on cancer cachexia in vivo. Levels of related signal proteins in both in vitro and in vivo experiments were examined using western blotting to study the possible mechanisms. Results Carnosol and its analogues [dimethyl‐carnosol (DCS) and dimethyl‐carnosol‐D6 (DCSD)] alleviated myotube atrophy of C2C12 myotubes and lipolysis of 3T3‐L1 adipocytes in vitro. Interestingly, CS and its analogues exhibited stronger inhibitive effects on muscle atrophy induced by tumour necrosis factor‐α (TNF‐α) (CS, P < 0.001; DCS, P < 0.001; DCSD, P < 0.001) in C2C12 myoblasts than on muscle atrophy induced by IL‐6 (CS, P < 0.05; DCS, P = 0.08; DCSD, P < 0.05). In a C26 tumour‐bearing mice model, administration of CS or its analogue DCSD significantly prevented body weight loss without affecting tumour size. At the end of the experiment, the body weight of mice treated with CS and DCSD was significantly increased by 11.09% (P < 0.01) and 11.38% (P < 0.01) compared with that of the C26 model group. CS and DCSD also improved the weight loss of epididymal adipose tissue in C26 model mice by 176.6% (P < 0.01) and 48.2% (P < 0.05) increase, respectively. CS and DCSD treatment partly preserved gastrocnemius myofibres cross‐sectional area. CS treatment decreased the serum level of TNF‐α (−95.02%, P < 0.01) but not IL‐6 in C26 tumour‐bearing mice. Inhibition on NF‐κB and activation of Akt signalling pathway were involved in the ameliorating effects of CS and its analogues on muscle wasting both in vitro and in vivo. CS and its analogues also alleviated adipose tissue loss by inhibiting NF‐κB and AMPK signalling pathways both in vitro and in vivo. Conclusions CS and its analogues exhibited anticachexia effects mainly by inhibiting TNF‐α/NF‐κB pathway and decreasing muscle and adipose tissue loss. CS and its analogues might be promising drug candidates for the treatment of cancer cachexia.

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Section: Methodsmentioning

confidence: 99%

Carnosol and its analogues attenuate muscle atrophy and fat lipolysis induced by cancer cachexia

Shen

et al. 2021

J cachexia sarcopenia muscle

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“…The exhaustive running test was carried out on eight racecourse small animal treadmills (SA101, Jiangsu Saions Biotechnology Co., Ltd) similar to what Shen et al. (2019) described. Except for the NC group, mice in the other groups took exercise after 1 h of gavage.…”

Section: Methodsmentioning

confidence: 99%

Camellia oil exhibits anti‐fatigue property by modulating antioxidant capacity, muscle fiber, and gut microbial composition in mice

Huang,

Sun,

Lin

et al. 2024

Journal of Food Science

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Camellia seed oil (CO) has high nutritional value and multiple bioactivities. However, the specific anti‐fatigue characteristics and the implied mechanism of CO have not yet been fully elucidated. Throughout this investigation, male C57BL/6J mice, aged 8 weeks, underwent exhaustive exercise with or without CO pretreatment (2, 4, and 6 mL/kg BW) for 28 days. CO could extend the rota‐rod and running time, reduce blood urea nitrogen levels and serum lactic acid, and increase muscle and hepatic glycogen, adenosine triphosphate, and anti‐oxidative indicators. Additionally, CO could upregulate the mRNA and Nrf2 protein expression levels, as well as enhance the levels of its downstream antioxidant enzymes and induce the myofiber‐type transformation from fast to slow and attenuate the gut mechanical barrier. Moreover, CO could ameliorate gut dysbiosis by reducing Firmicutes to Bacteroidetes ratio at the phylum level, increasing the percentage of Alistipes, Alloprevotella, Lactobacillus, and Muribaculaceae, and decreasing the proportion of Dubosiella at the genus level. In addition, specific bacterial taxa, which were altered by CO, showed a significant correlation with partial fatigue‐related parameters. These findings suggest that CO may alleviate fatigue by regulating antioxidant capacity, muscle fiber transformation, gut mechanical barrier, and gut microbial composition in mice.Practical ApplicationOur study revealed that camellia seed oil (CO) could ameliorate exercise‐induced fatigue in mice by modulating antioxidant capacity, muscle fiber, and gut microbial composition in mice. Our results promote the application of CO as an anti‐fatigue functional food that targets oxidative stress, myofiber‐type transformation, and microbial community.

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“…The mice in the exhaustive running group were subjected to the exhaustive running test, which was performed according to a previous study described by Shen (Shen et al, 2019). At the beginning of the experiment, the mice were given appropriate exercise training on a treadmill (SANS, Nanjing, China), and each group was subjected to an exhausted running test after the last gavage.…”

Section: Exhausted Running Testmentioning

confidence: 99%

Anti-fatigue activities of anthocyanins from Lycium ruthenicum Murry

et al. 2022

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Anthocyanins from Lycium ruthenicum Murry (LRAs) were used to explore the anti-fatigue activities of LRAs at different concentrations in a mouse fatigue model. Eight biochemical indexes were determined in liver tissue, muscle tissue and serum. The results showed that the LRAs used in the experiment mainly contained delphinium, petunidin and malvidin. During 21 days of gavage, there was no significant difference in mouse body weight. The exercise time of the LRA-exposed mice increased. LRAs could increase serum glucose (Glu), liver/muscle glycogen and the activity of superoxide dismutase (SOD) and reduce the vitality of lactate dehydrogenase (LDH), the content of malondialdehyde (MDA), the levels of lactic acid (LD) and serum urea nitrogen (BUN). This study indicated that LRAs can enhance exercise endurance, increase sugar reserves, scavenge free radicals and improve metabolism, thereby increasing anti-fatigue activities.

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SiBaoChongCao exhibited anti-fatigue activities and ameliorated cancer cachexia in mice

Abstract: SBCC exhibits anti-fatigue activity by improving muscle endurance and ameliorates cancer cachexia by alleviating loss of fat and body temperature.

Cited by 9 publications

References 54 publications

Carnosol and its analogues attenuate muscle atrophy and fat lipolysis induced by cancer cachexia

Carnosol and its analogues attenuate muscle atrophy and fat lipolysis induced by cancer cachexia

Camellia oil exhibits anti‐fatigue property by modulating antioxidant capacity, muscle fiber, and gut microbial composition in mice

Anti-fatigue activities of anthocyanins from Lycium ruthenicum Murry

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