Sialyllactose occurs as free lactones in ovine colostrum

Nakamura, Tadashi; Urashima, Tadasu; Nakagawa, Miyuki; Saito, Tadao

doi:10.1016/s0304-4165(98)00040-3

Cited by 37 publications

(32 citation statements)

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“…Urashima et al separated and purified oligosaccharides by dialysis, removal of proteins and a portion of the lactose by precipitation with ethanol, active carbon column chromatography, and paper chromatography, and characterized them using monosaccharide analysis, methylation analysis, and 13 C or 1 H-NMR spectroscopy. Nakamura et al 12,46) separated and purified acidic oligosaccharides by extraction of the carbohydrates with chloroform/methanol, gel filtration, anion exchange chromatography and normal phase HPLC with an Amide-80 column (Tosoh, Tokyo, Japan), and characterized them by 1 H-NMR. Based on these studies, 3…”

Section: Milk Oligosaccharides Of Domestic Farm Animals Other Thamentioning

confidence: 99%

“…It is to be hoped that a search of milk oligosaccharides and gangliosides in the milk/colostrum of domestic farm animals will yield components that can be used in the prevention of H. pylori infection. It has been suggested that Neu5Gc(2-3)Gal(1-4)Glc, found in bovine 6) and ovine colostrum, 46) inhibits the attachment of enterotoxigenic E. coli K99 to the piglet colonic mucosa, Neu5Gc(2-3)Gal being the receptor recognized by E. coli K99 adhesins. Gal(1-4)Gal and Gal(1-3)Gal(1-4)GlcNAc have been found to be the receptors recognized by Shigella toxin and a Shiga-like toxin produced by pathogenic E. coli, and toxin A produced by Clostridium difficile respectively.…”

Section: Future Industrial Utilization Of Milk Oligosaccharides mentioning

confidence: 99%

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Recent Advances in Studies on Milk Oligosaccharides of Cows and Other Domestic Farm Animals

Urashima

Taufik

Fukuda

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Section: Milk Oligosaccharides Of Domestic Farm Animals Other Thamentioning

confidence: 99%

Section: Future Industrial Utilization Of Milk Oligosaccharides mentioning

confidence: 99%

Recent Advances in Studies on Milk Oligosaccharides of Cows and Other Domestic Farm Animals

Urashima

Taufik

Fukuda

et al. 2013

Bioscience, Biotechnology, and Biochemistry

Self Cite

194

145

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“…Human colostrum and mature milk contain 22-24 g/l and 12-13 g/l of oligosaccharides, respectively; this oligosaccharide fraction is the third largest solid components, after lactose and lipid (Newburg and Neubauer, 1995). The oligosaccharide content of human milk is about 20-fold higher than that of bovine milk and is even higher than that of bovine colostrum (Veh et al, 1981;Nakamura et al, 1998). Human milk is also considered to be unique in its high content of complex fucosylated and sialylated oligosaccharides (Kunz et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Variation of major neutral oligosaccharides levels in human colostrum

et al. 2007

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Objectives: Human colostrum is known to be important for the protection of infants against infection by pathogenic microorganisms. This protection is thought to be due, partially, to various neutral and acidic oligosaccharides that are present in colostrum and milk. However, the concentrations of each of the oligosaccharide of human colostrum have not yet been determined. The aim of this present study was to determine the concentration of each of the major neutral oligosaccharide for three consecutive days from the start of lactation. Method: We analyzed the level of each neutral oligosaccharide in human colostrum, for three consecutive days from the start of lactation, obtained from 12 healthy Japanese women (ranging in age from 21 to 35 years; primipara 6 and multipara 6). The ABO blood groups of the donors were determined: A, three; B, three; O, five; AB, one. The determined human milk oligosaccharides were 2 0 -fucosyllactose (2 0 -FL), 3-fucosyllactose (3-FL), lactodifucotetraose (LDFT), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT), three lacto-N-fucopentaose (LNFP I, II and III) and two lacto-N-difucohexaose (LNFDH I and II) using high-performance liquid chromatography (HPLC) with two derivatization techniques. Results: The concentrations of 2 0 -FL and LDFT in colostrum on day 1 were significantly higher than those on days 2 and 3 (Po0.05). An increase in LNT was observed on day 3 compared with day 1 (Po0.05). Conclusion: These changes in concentrations of 2'-FL, LDFT and LNT may reflect the requirements for prebiotics and antiinfection agents by human infants during early lactation.

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“…Another study has shown that mature milk from Spanish goats contains 6'-SL, 3'-SL, disialyllactose, Nglycolylneuraminyllactose, 3'-galactosyllactose, N-acetyl glucosaminyllactose, LNH and additional high molecular oligosaccharides, as demonstrated by analysis with FAB-MS, but no fucosyl oligosaccharides (MartinezFerez et al, 2006). Ovine colostrum contains more 3'-N-glycolylneuraminyllactose than 3'-SL and 6'-SL (Nakamura et al, 1998) and, notably, contains Neu5Gc in preference to Neu5Ac.…”

Section: Milk Oligosaccharides Of the Cow And Other Domestic Farm Animentioning

confidence: 93%

“…Human colostrum and mature milk contain 22-24 g/l and 12-13 g/l of oligosaccharides, respectively; this oligosaccharide fraction is the third largest solid components, after lactose and lipid (Newburg & Neubauer, 1995). The oligosaccharide content of human milk is about 20-fold higher than that of bovine milk and is even higher than that of bovine colostrum (Veh et al, 1981;Nakamura et al, 1998). Human milk is also considered to be unique in its high content of complex fucosylated and sialylated (HPLC) -chip mass spectrometry (MS) (Ninonuevo et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Oligosaccharides in Milk: Their Benefits and Future Utilization

Urashima¹,

Taufik²

2010

medpet

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The percentage of carbohydrate in the milk/colostrum of the mammalian is range from trace to over 10%, of which disaccharide lactose (Gal(β1-4)Glc) is usually constitutes the major part. Apart from the lactose (Gal(β1-4)Glc; Gal, D-galactose; Glc, D-glucose), the rest of carbohydrate components is composed of variety of sugars, commonly named as milk oligosaccharides. Human mature milk and colostrum contain 12 ~ 13 g/l and 22 ~ 24 g/l of oligosaccharides, respectively. In contrast, bovine colostrum contains more than 1 g/l oligosaccharides and this concentration rapidly decreases after 48 hr post partum. Most of human milk oligosaccharides (HMO) are resistant to digestion and absorption within the small intestine. Therefore they can reach the infant colon, where they can act as prebiotics that stimulate the growth of benefi cial microorganisms such as various species of Bifi dobacterium. They can also act as receptor analogues that inhibit the a achment of pathogenic microorganisms to the infant's colonic mucosa. A small part of the milk oligosaccharides is absorbed intact into the circulation and it has been hypothesized that these may act as immunomodulators. Generally, the bovine milk oligosaccharides are believed not to be absorbed by human adults or infants, thus making them available to be utilized as prebiotics or anti-infection materials. The colostrum of cows and other domestic farm animals is a potential source of free oligosaccharides, and oligosaccharides isolated from these natural sources can be utilized as functional foods or animal feedstuff s on the industrial scale.Key words: milk, oligosaccharides, prebiotic, Bifi dobacterium * Corresponding author: e-mail: urashima@obihiro.ac.jp oligosaccharides . In this review, we will introduce the structural feature of human milk oligosaccharides, on their fate within the gastrointestinal tract as well as on their possible biological functions as prebiotics, anti infection agents and immunomodulation factors. In addition, we will discuss the possibility of the commercial utilization of bovine milk oligosaccharides and those of other domestic farm animals. THE CHEMICAL STRUCTURES AND THEIR QUANTITATIVE ASPECTS OF HUMAN MILK OLIGOSACCHARIDESThe structures of at least 115 human milk oligosaccharides (HMOs) have been determined to date (Urashima et al., in press), while as many as 200 diff erent oligosaccharides have been separated and studied by microfl uidic high performance liquid chromatography

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Sialyllactose occurs as free lactones in ovine colostrum

Cited by 37 publications

References 23 publications

Recent Advances in Studies on Milk Oligosaccharides of Cows and Other Domestic Farm Animals

Recent Advances in Studies on Milk Oligosaccharides of Cows and Other Domestic Farm Animals

Variation of major neutral oligosaccharides levels in human colostrum

Oligosaccharides in Milk: Their Benefits and Future Utilization

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