Sialic Acid-Targeted Biointerface Materials and Bio-Applications

Xiong, Yüting; Li, Minmin; Lü, Qing; Qing, Guangyan; Sun, Taolei

doi:10.3390/polym9070249

Cited by 26 publications

(15 citation statements)

References 84 publications

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“…MPC was purchased from NOF Co., Tokyo, Japan, which was synthesized using a previously reported procedure. [ 11,21 ] n ‐Butyl methacrylate (BMA) and fibronectin were obtained from FUJI FILM Wako Pure Chemical Co, Ltd. (Osaka, Japan). p ‐Vinylphenylboronic acid (VPBA) and α,α' ‐azobisisobutyronitrile (AIBN) were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

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Control of Cell‐Substrate Binding Related to Cell Proliferation Cycle Status Using a Cytocompatible Phospholipid Polymer Bearing Phenylboronic Acid Groups

Ishihara

Abe

Fukazawa

et al. 2021

Macromolecular Bioscience

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To provide high‐quality cellular raw materials for cell engineering and pharmaceutical engineering, a polymer substrate is prepared for cell separation focusing on the cell proliferation cycle. There are many types of sugar chains on cell membranes, which function as signaling molecules to control interactions with the exterior of the cell; their abundance changes during the cell‐proliferation cycle. In this study, a phenylboronic acid group, which has affinity for sugar chains, is introduced into a polymer containing a phosphorylcholine group that does not induce cell activation. On the surface of this polymer, human promyelocytic leukemia cells can adhere. The adhesion rate is increased by pretreating the substrate with an alkaline solution. Moreover, cell adhesion is dependent on the sugar additive in the culture medium. Therefore, cell adhesion is governed by reactions between the sugar chain on the cell membrane and the phenylboronic acid groups on the substrate. It is revealed that the adhesion rate changes depending on the expression level of sugar chains related to the cell‐proliferation cycle. Based on this, it may be proposed a cell proliferation cycle‐specific separation process using the polymer substrate based on cell adhesion depending on sugar chain density.

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Section: Methodsmentioning

confidence: 99%

“…The cell binding can be detected through the selective reactions. [ 20–23 ] Based on these results, we hypothesized that cells bearing sugar chains could be captured on the surface of the MPC polymer via phenylboronic acid groups. The reaction between sugar and phenylboronic acid is reversible and depends on the binding constant.…”

Section: Introductionmentioning

confidence: 99%

Control of Cell‐Substrate Binding Related to Cell Proliferation Cycle Status Using a Cytocompatible Phospholipid Polymer Bearing Phenylboronic Acid Groups

Ishihara

Abe

Fukazawa

et al. 2021

Macromolecular Bioscience

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“…Commercially available boronic acids bind sialic acid residues [177]. For that reason, they can be employed to target MSNs to HepG2 cells showing aberrant overexpression of such residues [178].…”

Section: Small Moleculesmentioning

confidence: 99%

Influence of the Surface Functionalization on the Fate and Performance of Mesoporous Silica Nanoparticles

Gisbert-Garzarán

Vallet‐Regí

2020

Nanomaterials

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Mesoporous silica nanoparticles have been broadly applied as drug delivery systems owing to their exquisite features, such as excellent textural properties or biocompatibility. However, there are various biological barriers that prevent their proper translation into the clinic, including: (1) lack of selectivity toward tumor tissues, (2) lack of selectivity for tumoral cells and (3) endosomal sequestration of the particles upon internalization. In addition, their open porous structure may lead to premature drug release, consequently affecting healthy tissues and decreasing the efficacy of the treatment. First, this review will provide a comprehensive and systematic overview of the different approximations that have been implemented into mesoporous silica nanoparticles to overcome each of such biological barriers. Afterward, the potential premature and non-specific drug release from these mesoporous nanocarriers will be addressed by introducing the concept of stimuli-responsive gatekeepers, which endow the particles with on-demand and localized drug delivery.

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“…4 -Carboxyphenylboronic acid (4cPBA) was coupled to the amine functions of PAH by an EDC/NHS coupling reaction. 4cPBA is able to bind selectively to sialic acid at physiological pH [40] and has been used by other groups to selectively target sialic acid on cells and tissues [26,[41][42][43]. During the coupling step, the average hydrodynamic diameter increased from 74 ± 2 nm to 75.6 ± 2 nm.…”

Section: (B) Functionalization Of Sers Nanoprobesmentioning

confidence: 99%

Multiplex micro-SERS imaging of cancer-related markers in cells and tissues using poly(allylamine)-coated Au@Ag nanoprobes

et al. 2020

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Surface-enhanced Raman scattering (SERS) nanoprobes based on Au@Ag core@shell nanoparticles coated with poly(allylamine) were functionalized with small targeting molecules to evaluate simultaneously the level of expression of two cancer-related markers, both in cells and in tissues. The Au@Ag nanoparticles provide a high SERS signal enhancement in the visible range when combined with resonant Raman-active molecules. The poly(allylamine) coating plays a dual key role in (i) protecting the metal surface against the complex biological medium, leading to a stable signal of the Raman-active molecules, and (ii) enabling specific biofunctionalization through its amine functions. Using small targeting molecules linked to the polymer coating, two different nanoprobes (duplex approach) were designed. Each was able to specifically target a particular cancerrelated marker: folate receptors (FRs) and sialic acid (SA). We demonstrate that the level of expression of these targeted markers can be evaluated following the SERS signal of the probes incubated on cells or tissues. The potential overexpression of folate receptors and of sialic acid was evaluated and measured in breast and ovarian cancerous tissue sections. In addition, FR and/or SA overexpression in the tumor region can be visualized with high contrast with respect to the healthy region and with high spatial accuracy consistent with histology by SERS imaging of the nanoprobe signal. Owing to the unique spectral signature of the designed nanoprobes, this approach offers an efficient tool for the spatially resolved, in situ measurement of the expression level of several cancer-related markers in tumors at the same time.

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Sialic Acid-Targeted Biointerface Materials and Bio-Applications

Cited by 26 publications

References 84 publications

Control of Cell‐Substrate Binding Related to Cell Proliferation Cycle Status Using a Cytocompatible Phospholipid Polymer Bearing Phenylboronic Acid Groups

Control of Cell‐Substrate Binding Related to Cell Proliferation Cycle Status Using a Cytocompatible Phospholipid Polymer Bearing Phenylboronic Acid Groups

Influence of the Surface Functionalization on the Fate and Performance of Mesoporous Silica Nanoparticles

Multiplex micro-SERS imaging of cancer-related markers in cells and tissues using poly(allylamine)-coated Au@Ag nanoprobes

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