Shrimp sensitization in house dust mite allergic patients

Ukleja-Sokołowska, Natalia; Gawrońska-Ukleja, Ewa; Lis, Kinga; Żbikowska-Gotz, Magdalena; Adamczak, Rafał; Sokołowski, Łukasz; Bartuzi, Zbigniew

doi:10.1177/2058738420907188

Cited by 5 publications

(10 citation statements)

References 11 publications

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“…As observed in our previous study, there was high concordance of sIgE against Der p 10 and Pen m 1 (ImmunoCap) (Spearman correlation 0.918, p < 0.001), which may indicate that it is economically sensible to diagnose shrimp allergy using only one of the methods. 25 We also found positive correlations between concentrations of sIgE to Der p 1, Der p 2 and Der p 10 by singleplex ImmunoCap and the corresponding allergen components in ImmunoCap ISAC. Not all patients with elevated sIgE according to the singleplex ImmunoCap method were detected, but the detection rate was above 84% in both cases.…”

Section: Discussionsupporting

confidence: 61%

Clinical utility of immunological methods based on the singleplex and multiplex ImmunoCap systems for diagnosis of shrimp allergy

et al. 2021

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Background Levels of specific IgE (sIgE) against allergen components can be assessed using multiplex assays or with highly sensitive, quantitative methods. The aim of this study was to compare the sensitivity and specificity of different immunological methods for diagnosis of shrimp allergy. Methods Twenty patients with positive skin prick tests for frozen tiger shrimp were selected for further examination. Blood samples were taken to assess concentrations of sIgE against the house dust mites Dermatophagoides pteronyssinus and D. farinae, shrimp allergen extract, allergen components Der p 1, Der p 2 and Pan a 1 (ImmunoCap), and the ImmunoCap ISAC 112 panel. Results All patients had elevated levels of sIgE against shrimp and D pteronyssinus. Eight patients were sensitized to Pen m 1, three patients were sensitized to Pen m 2, and two patients were sensitized to Pen m 4 (ISAC). ImmunoCap ISAC detected shrimp sensitization in 50% of patients. There was a strong correlation between concentrations of sIgE against Pen m1 and Der p 10 detected by ImmunoCap. Conclusions The singleplex ImmunoCap system remains the reference diagnostic method, but in the case of shrimp allergy ImmunoCap ISAC provided better insight into patient allergen profiles.

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Section: Discussionsupporting

confidence: 61%

Clinical utility of immunological methods based on the singleplex and multiplex ImmunoCap systems for diagnosis of shrimp allergy

et al. 2021

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“…In our previous studies based on the assessment of the concentrations of sIgE Der p 10 and Pen a 1 in the quantitative ImmunoCap method, the correlation coefficient between these concentrations was as much as 0.987. This suggested that it was economically unjustified to use both determinations, as the results practically coincided [19]. In the present study, the use of the semiquantitative ImmunoCap ISAC method, characterized by lower sensitivity, resulted in a significantly greater discrepancy between results.…”

Section: Discussionmentioning

confidence: 50%

“…In that study population, 86 (90.5%) patients had positive skin prick tests for house dust mite allergens [18]. As our previous observations indicated, among 232 patients with symptoms of allergic rhinitis and as many as 59 patients had elevated levels of shrimp sIgE, while only 12 were not allergic to house dust mites (20.3%) [19]. The differences may be due to a different population selection criterion, however, the high frequency of mite allergy in patients allergic to shrimp allergens is confirmed.…”

Section: Discussionmentioning

confidence: 51%

Assessment of TSLP, IL 25 and IL 33 in patients with shrimp allergy

Ukleja-Sokołowska

Żbikowska-Gotz

Lis

et al. 2021

Allergy Asthma Clin Immunol

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Background Shrimp allergy is a growing problem among the European population. TSLP, IL-25 and IL-33 are involved in the pathophysiology of allergic diseases, including asthma and atopic dermatitis, as they activate the Th2-dependent immune response. Methods Thirty-seven patients (18 male and 19 female) with a positive history of symptoms associated with shrimp consumption were selected. All patients had blood samples taken to assess the concentration of allergen-specific IgE (sIgE) to house dust mites (HDM) and shrimp (Singleplex, quantitative method with cut off value > 0,35 kAU/L) as well as the level of allergen components using the ImmunoCap ISAC method (Microarray test, semi-quantitative with cut off value > 0,3 ISU-E). The concentrations of TSLP, IL-25 and IL-33 in the patients’ blood serum was assessed using the ELISA method (Cusabio). Twenty patients with negative allergy history of allergic disease tests were included in the control group. Results Among the 37 shrimp-allergic patients, ImmunoCap ISAC was identified the presence of sIgE to the available shrimp allergen components in only 14 cases (37.8%). TSLP and IL25 levels were significantly higher in the study group. No statistically significant correlation was found between the concentration of analyzed alarmins and the concentration of sIgE level to shrimp or HDM between the study and control groups. No statistically significant correlation was found between poly-sensitization occurring in patients and levels of TSLP, IL-25 and IL-33 . Conclusion In shrimp-allergic patients, the concentrations of TSLP and IL-25 were significantly higher than in the control group (1.33 vs. 0.49 and 157 vs. 39.36, respectively). There was no correlation between the concentrations of TSLP, IL-25 and IL-33 and the concentration of sIgE in the patients or the number of allergen components that the patients were sensitized to. Trial registration: Bioethics Committee 147/2015, 11.03.2015.

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“…Our study is the first to describe molecular sensitization patterns to HDM allergens in a Lithuanian population and to highlight the role played by age and gender, while simultaneously analyzing the phenomenon of concomitant reactivity among different allergens. [18][19][20] Immediate hypersensitivity to indoor allergens increases the risk of asthma, allergic rhinitis, and atopic dermatitis. HDM sensitization is globally recognized as the leading cause of inhaled allergen-triggered allergies.…”

Section: Discussionmentioning

confidence: 99%

Sensitization profiles to house dust mite Dermatophagoides pteronyssinus molecular allergens in the Lithuanian population: Understanding allergic sensitization patterns

Biliute,

Miskinyte,

Miskiniene

et al. 2024

Clinical & Translational All

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BackgroundHouse dust mite (HDM) allergy is a prevalent global health concern, with varying sensitization profiles observed across populations. We aimed to provide a comprehensive assessment of molecular allergen sensitization patterns in the Lithuanian population, with a focus on Dermatophagoides pteronyssinus (Der p), and investigate patterns of concomitant reactivity among different allergens to enhance the accuracy of HDM allergy diagnostics.MethodsA comprehensive analysis of 1520 patient test results in Lithuania from 2020 to 2022 was performed. Sensitization patterns to major (Der p 1, Der p 2, and Der p 23) and minor (Der p 5, Der p 7, and Der p 21) Der p allergen components were described using molecular‐based diagnostics. Additionally, we investigated sensitization to allergen components from other allergen sources, including tropomyosins (Der p 10, Per a 7, Pen m 1, Ani s 3, Blo t 10) and arginine kinases (Pen m 2, Bla g 9, Der p 20).ResultsThis study reveals a high prevalence of HDM sensitization in Lithuania ‐ 481 individuals (45.38% of the sensitized group) exhibited sensitization to at least one Der p allergen component. Importantly, within the sensitized group, 37.21% of patients were sensitized to Der p 5, Der p 7, or Der p 21 in addition to major allergenic components. Distinct sensitization patterns were observed across different age groups, indicating the influence of age‐related factors. Furthermore, we confirmed cross‐reactivity between Der p 5 and Blo t 5 as well as between Der p 21 and Blo t 21, emphasizing the clinical relevance of these associations. We also highlighted the complexity of sensitization patterns among tropomyosins and arginine kinases.ConclusionThis study provides valuable insights into HDM allergy sensitization profiles in Lithuania, emphasizing the importance of considering major and minor HDM allergen components for accurate diagnosis and management of HDM‐related allergic diseases. Differences between populations and age‐related factors impact sensitization patterns. Understanding concomitant reactivity among allergens, such as Der p 5 and Blo t 5, Der p 21 and Blo t 21, tropomyosins, and arginine kinases, is crucial for improving diagnostic strategies and developing targeted interventions for allergic individuals.

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Shrimp sensitization in house dust mite allergic patients

Cited by 5 publications

References 11 publications

Clinical utility of immunological methods based on the singleplex and multiplex ImmunoCap systems for diagnosis of shrimp allergy

Clinical utility of immunological methods based on the singleplex and multiplex ImmunoCap systems for diagnosis of shrimp allergy

Assessment of TSLP, IL 25 and IL 33 in patients with shrimp allergy

Sensitization profiles to house dust mite Dermatophagoides pteronyssinus molecular allergens in the Lithuanian population: Understanding allergic sensitization patterns

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