Clinical utility of immunological methods based on the singleplex and multiplex ImmunoCap systems for diagnosis of shrimp allergy

Ukleja-Sokołowska, Natalia; Lis, Kinga; Żbikowska-Gotz, Magdalena; Adamczak, Rafał; Kuźmiński, Andrzej; Bartuzi, Zbigniew

doi:10.1177/03000605211006597

Cited by 4 publications

(4 citation statements)

References 20 publications

(30 reference statements)

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“…This indicates that although component diagnostics based on the ImmunoCap ISAC test is important in assessing the allergen profile of patients, in the case of shrimp allergy, it plays an auxiliary role, and a negative result in the context of the allergen components Pen m 1, Pen m 2 and Pen m 4 does not rule out the shrimp allergy. In our previous research, we found that, in case of shrimp sensitization, the results of sIgE levels against corresponding allergen components determined using singleplex ImmunoCap (quantitative and highly sensitive) and ImmunoCap ISAC (semi-quantitative, multiplex micro-assay test) are similar in most cases, with correlations above 0.7 [22].…”

Section: Discussionmentioning

confidence: 86%

Assessment of TSLP, IL 25 and IL 33 in patients with shrimp allergy

Ukleja-Sokołowska

Żbikowska-Gotz

Lis

et al. 2021

Allergy Asthma Clin Immunol

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Background Shrimp allergy is a growing problem among the European population. TSLP, IL-25 and IL-33 are involved in the pathophysiology of allergic diseases, including asthma and atopic dermatitis, as they activate the Th2-dependent immune response. Methods Thirty-seven patients (18 male and 19 female) with a positive history of symptoms associated with shrimp consumption were selected. All patients had blood samples taken to assess the concentration of allergen-specific IgE (sIgE) to house dust mites (HDM) and shrimp (Singleplex, quantitative method with cut off value > 0,35 kAU/L) as well as the level of allergen components using the ImmunoCap ISAC method (Microarray test, semi-quantitative with cut off value > 0,3 ISU-E). The concentrations of TSLP, IL-25 and IL-33 in the patients’ blood serum was assessed using the ELISA method (Cusabio). Twenty patients with negative allergy history of allergic disease tests were included in the control group. Results Among the 37 shrimp-allergic patients, ImmunoCap ISAC was identified the presence of sIgE to the available shrimp allergen components in only 14 cases (37.8%). TSLP and IL25 levels were significantly higher in the study group. No statistically significant correlation was found between the concentration of analyzed alarmins and the concentration of sIgE level to shrimp or HDM between the study and control groups. No statistically significant correlation was found between poly-sensitization occurring in patients and levels of TSLP, IL-25 and IL-33 . Conclusion In shrimp-allergic patients, the concentrations of TSLP and IL-25 were significantly higher than in the control group (1.33 vs. 0.49 and 157 vs. 39.36, respectively). There was no correlation between the concentrations of TSLP, IL-25 and IL-33 and the concentration of sIgE in the patients or the number of allergen components that the patients were sensitized to. Trial registration: Bioethics Committee 147/2015, 11.03.2015.

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Section: Discussionmentioning

confidence: 86%

Assessment of TSLP, IL 25 and IL 33 in patients with shrimp allergy

Ukleja-Sokołowska

Żbikowska-Gotz

Lis

et al. 2021

Allergy Asthma Clin Immunol

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“…The results were narratively synthesized. For consistency, data in the synthesis were limited to measurements with the conventional 32 , 33 , 34 cut‐off values of 0.35 kUA/l for ImmunoCAP and 0.3 ISU for ISAC. Meta‐analysis was not possible to perform, given the insufficient and disparate data.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of sensitization to molecular food allergens in Europe: A systematic review

Lisik

Ioannidou

Spolidoro

et al. 2022

Clinical & Translational All

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Background Recent reports indicate that the prevalence of food allergy is increasing, but accurate estimates remain a challenge due to cross‐reactivity and limited use of precise diagnostic methods. Molecular allergy diagnostics, in which sensitization to individual molecular allergens is measured, is emerging as a promising tool for evaluation of sensitization profiles. In this systematic review, we summarized estimates of prevalence of sensitization to molecular food allergens in the general population in Europe. Methods Following a protocol prospectively registered with the International Prospective Register of Systematic Reviews (PROSPERO; reference CRD42021266657), we searched seven databases with no restrictions on publication date or language. Two reviewers independently screened the literature, extracted data, and appraised the risk of bias in the included studies. The findings were synthesized narratively. Results From 4776 de‐duplicated records, five studies, with low to moderate overall risk of bias, were included. Forty‐six molecular allergens from 18 foods were investigated. Overall, the prevalence of sensitization was low, particularly for major allergens, and non‐existent for 10 molecular allergens (0% [95% CI 0–0.8]). The highest prevalence was seen for PR‐10 proteins, such as Cor a 1.04 (13.6% [95% CI 10.9–16.9]). Conclusions Available data, primarily from North‐western Europe, indicate that sensitization to molecular food allergens is overall low. The highest estimates were found for cross‐reactive PR‐10 proteins. There were not enough studies to discern regional differences or perform meta‐analysis, highlighting the need for more population‐representative studies in order to elucidate patterns of sensitization to molecular food allergens in Europe.

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“…ImmunoCAP detected elevated levels of shrimp sIgE in all patients. However, ISAC 112 indicated only a 50% detection rate against at least one out of three shrimp allergen components [ 123 ].…”

Section: Diagnosismentioning

confidence: 99%

IgE-Mediated Shellfish Allergy in Children

et al. 2023

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Shellfish, including various species of mollusks (e.g., mussels, clams, and oysters) and crustaceans (e.g., shrimp, prawn, lobster, and crab), have been a keystone of healthy dietary recommendations due to their valuable protein content. In parallel with their consumption, allergic reactions related to shellfish may be increasing. Adverse reactions to shellfish are classified into different groups: (1) Immunological reactions, including IgE and non-IgE allergic reactions; (2) non-immunological reactions, including toxic reactions and food intolerance. The IgE-mediated reactions occur within about two hours after ingestion of the shellfish and range from urticaria, angioedema, nausea, and vomiting to respiratory signs and symptoms such as bronchospasm, laryngeal oedema, and anaphylaxis. The most common allergenic proteins involved in IgE-mediated allergic reactions to shellfish include tropomyosin, arginine kinase, myosin light chain, sarcoplasmic calcium-binding protein, troponin c, and triosephosphate isomerase. Over the past decades, the knowledge gained on the identification of the molecular features of different shellfish allergens improved the diagnosis and the potential design of allergen immunotherapy for shellfish allergy. Unfortunately, immunotherapeutic studies and some diagnostic tools are still restricted in a research context and need to be validated before being implemented into clinical practice. However, they seem promising for improving management strategies for shellfish allergy. In this review, epidemiology, pathogenesis, clinical features, diagnosis, and management of shellfish allergies in children are presented. The cross-reactivity among different forms of shellfish and immunotherapeutic approaches, including unmodified allergens, hypoallergens, peptide-based, and DNA-based vaccines, are also addressed.

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Clinical utility of immunological methods based on the singleplex and multiplex ImmunoCap systems for diagnosis of shrimp allergy

Cited by 4 publications

References 20 publications

Assessment of TSLP, IL 25 and IL 33 in patients with shrimp allergy

Assessment of TSLP, IL 25 and IL 33 in patients with shrimp allergy

Prevalence of sensitization to molecular food allergens in Europe: A systematic review

IgE-Mediated Shellfish Allergy in Children

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