Shrimp arginine kinase being a binding protein of WSSV envelope protein VP31

Ma, Cuiyan; Gao, Qiang; Liang, Yan; Li, Chen; Liu, Chao; Huang, Jie

doi:10.1007/s00343-016-5198-7

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…Moreover, in the closely related mollicute-leafhopper association (C. haematoceps/Spiroplasma citri), hexamerin is upregulated following infection and is required for vector survival after spiroplasma inoculation (36). Besides their role in energy metabolism (see below), arthropod arginine kinases (AK) are also involved in stress response and innate immunity; Apis cerana AK is induced by abiotic and biotic stresses (39), pacific oyster AK modulates bactericidal immune response in hemolymph (40), and AK from Fenneropenaeus chinensis shrimp has been hypothesized to be the putative receptor of the WSSV virus envelope protein (41). AK transcript was upregulated upon CYp infection, suggesting different potential roles for this protein during infection with the two phytoplasmas.…”

Section: Discussionmentioning

confidence: 99%

Two Phytoplasmas Elicit Different Responses in the Insect Vector Euscelidius variegatus Kirschbaum

et al. 2018

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Phytoplasmas are plant-pathogenic bacteria transmitted by hemipteran insects. The leafhopper is a natural vector of chrysanthemum yellows phytoplasma (CYp) and a laboratory vector of flavescence dorée phytoplasma (FDp). The two phytoplasmas induce different effects on this species: CYp slightly improves whereas FDp negatively affects insect fitness. To investigate the molecular bases of these different responses, transcriptome sequencing (RNA-seq) analysis of infected with either CYp or FDp was performed. The sequencing provided the first transcriptome assembly for a phytoplasma vector and a starting point for further analyses on differentially regulated genes, mainly related to immune system and energy metabolism. Insect phenoloxidase activity, immunocompetence, and body pigmentation were measured to investigate the immune response, while respiration and movement rates were quantified to confirm the effects on energy metabolism. The activation of the insect immune response upon infection with FDp, which is not naturally transmitted by, confirmed that this bacterium is mostly perceived as a potential pathogen. Conversely, the acquisition of CYp, which is naturally transmitted by , seems to increase the insect fitness by inducing a prompt response to stress. This long-term relationship is likely to improve survival and dispersal of the infected insect, thus enhancing the opportunity of phytoplasma transmission.

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Section: Discussionmentioning

confidence: 99%

Two Phytoplasmas Elicit Different Responses in the Insect Vector Euscelidius variegatus Kirschbaum

et al. 2018

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“…Among them, VP37 is an important envelope protein of WSSV [ 19 ], which plays a major role in WSSV infection [ 20 ], and the recombinant VP37 has shown a high binding activity with the shrimp cell membrane in a binding assay [ 21 ]. VP31 is also an important viral envelope protein that might be involved in WSSV infection [ 22 ], and was identified as being able to interact with several proteins in shrimp [ 23 , 24 ]. VP110 was able to bind to the surface of crayfish haemocyte, and the synthetic RGDT peptide was able to inhibit attachment of VP110 to the cell membrane [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

The Roles of β-Integrin of Chinese Shrimp (Fenneropenaeus chinensis) in WSSV Infection

Tang

Zhai

Sheng

et al. 2017

IJMS

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Our previous study demonstrated that an integrin β subunit of Chinese shrimp (Fenneropenaeus chinensis) (FcβInt) plays an important role in white spot syndrome virus (WSSV) infection. In the present work, in order to further elucidate the potential role of FcβInt in WSSV infection, the recombinant extracellular domain of β integringene of F. Chinensis (rFcβInt-ER) was expressed in Escherichia coli BL21 (DE3), and the eukaryotic expression plasmid PcDNA3.1-FcβInt-ER (PFcβInt-ER) was also constructed. Far-western blotting was performed to determine the binding specificity of rFcβInt-ER to WSSV envelope proteins, and results showed that rFcβInt-ER was able to specifically interact with rVP31, rVP37, rVP110 and rVP187. Moreover, the blocking effects of mouse anti-rFcβint-ER antibodies were both detected in vivo and in vitro. The ELISA and Dot-blotting in vitro assays both showed that mouse anti-rFcβInt-ER antibodies could partially block the binding of WSSV to the hemocyte membrane of F. chinensis. In the in vivo assays, the mortality of shrimp injected with WSSV mixed with anti-rFcβInt-ER antibodies was delayed, and was lower than in the control group. While the shrimp were intramuscularly injected with PFcβInt-ER, transcripts of PFcβInt-ER could be detected in different shrimp tissues within 7 days, and the mortality of shrimp injected with PFcβInt-ER was also delayed and lower compared with the control group post WSSV challenge. Furthermore, gene silencing technology was also used to verify the effect of FcβInt in WSSV infection, and results showed that the expression levels of the WSSV immediate early gene iel, early gene wsv477, and late gene VP28 and the mortality of F. Chinensis were all significantly decreased in the FcβInt knock-down hemocyctes compared to the control group. Taken together, these results suggest that FcβInt plays important roles in WSSV infection.

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“…ORFwsv230 is a nonstructural protein suspected to be involved in viral replication, viral particle assembly and inhibition of host cell functions (Liu et al 2006;S anchez-Paz 2010). VP26 was shown to interact with actin fibres and is associated with the nucleocapsid cytoplasmic transport (Xie & Yang, 2005;Ma et al 2016). ORFwsv89 encodes nearly 165-kDa protein, localized in the host nucleus might function as transcription regulator and can repress its promoter as well as the kinase and thymidine-thymidylate kinase of WSSV and sought to modulate viral gene expression during dormant state (Tsai et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Challenges and prospects of Viral Envelope protein VP28‐based control strategies to combat white spot syndrome virus in penaeid shrimps: a review

Chandrika

Puthiyedathu

2020

Reviews in Aquaculture

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VP28 is an essential WSSV envelope protein used to develop innate immune recognition in shrimp against WSSV, owing to its remarkable capability to localize on the surface of shrimp epithelial cells. Transgenically engineered viral VP28 protein from several expression systems was used to increase the survival chances of shrimp against WSSV pathogen, and their efficacy was found to last for 2-3 weeks following therapy. Though few reports have shown that DNA vaccination using the VP28 DNA construct is capable of protecting shrimp from WSSV, the exact mechanism behind the immunity is still unknown. Several research groups have provided insights into VP28-driven dsRNA vaccination to protect shrimp against WSSV infection, but the stability attained by many of these vaccines turned out to be short-lived. RNAi treatment with VP28dsRNA, shrimp showed better resistance to the WSSV challenge, although multiple doses of dsRNA are required to provide extended protection for up to 28 days. However, no foolproof protection was currently offered by VP28-based recombinant protein or DNA vaccination against WSSV. In recent years, attention has been directed towards identifying small molecule inhibitors of VP28 through molecular docking and molecular dynamics simulation studies and is an emerging approach in drug development and design against WSSV. This review highlights the opportunities, limits and challenges of VP28-based control strategies of White Spot Syndrome Virus, by emphasizing the status quo of current methodological approaches and suggesting future research directions of using it as a potential drug discovery target.

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Shrimp arginine kinase being a binding protein of WSSV envelope protein VP31

Cited by 4 publications

References 40 publications

Two Phytoplasmas Elicit Different Responses in the Insect Vector Euscelidius variegatus Kirschbaum

Two Phytoplasmas Elicit Different Responses in the Insect Vector Euscelidius variegatus Kirschbaum

The Roles of β-Integrin of Chinese Shrimp (Fenneropenaeus chinensis) in WSSV Infection

Challenges and prospects of Viral Envelope protein VP28‐based control strategies to combat white spot syndrome virus in penaeid shrimps: a review

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