Show Yourself, Asparaginase: An Enzymatic Reaction Explained through a Hands-On Interactive Activity

Ramírez‐Paz, Josell; Ortiz-Andrade, Bonny M.; Griebenow, Kai; Díaz-Vázquez, Liz M.

doi:10.1021/acs.jchemed.6b00612

Cited by 5 publications

(5 citation statements)

References 19 publications

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“…Using a pharmaceutically relevant protein, the authors of the other research 55 brought the concept of enzymatic activity to the classroom. They designed a hands-on interactive activity to promote the emerging field of protein-based drugs as a source of scientific careers in nanobiotechnology and to show the students an image of "scientist" as an ordinary person working in an exciting profession.…”

Section: High Impact Research and Trending Topics In The Biochemistry...mentioning

confidence: 99%

Mapping research on biochemistry education: A bibliometric analysis

Barbosa

Galembeck

2022

Biochem Molecular Bio Educ

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The purpose of this study was to map the research literature on Biochemistry education, covering the scientific production indexed on the Web of Science over the past 66 years. The open-source Bibliometrix R-package, an R-tool, was used to carry out the bibliometric analysis. Our results describe (1) how many articles were published per year and what is the annual average growth rate; (2) which are the core journals, authors, and publications in the field;(3) which countries and funding agencies contribute most to the development of research in the area; (4) the leading collaborative research and co-citation networks; (5) which articles were the most cited in the past 10 years; and (6) which are the trending topics in the field. Our main contribution is offering insights into the evolution of the field. Also, the use of a quantitative methodological design, which covers a large volume of publications, and could identify possible gaps in the area.

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Section: High Impact Research and Trending Topics In The Biochemistry...mentioning

confidence: 99%

Mapping research on biochemistry education: A bibliometric analysis

Barbosa

Galembeck

2022

Biochem Molecular Bio Educ

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“…If the enzyme is attached to a nonporous material, mass transfer limitations occur only because of the stagnant film, or Nernst layer surrounding the support material. Should the enzyme be entrapped inside a porous matrix, internal mass transfer limitations come into play as well. , Numerous experiments on enzyme catalysis have already been published in this Journal , focusing on fundamental and applied aspects of biocatalysis. − While immobilization has been tackled in diverse reports, − to the best of our knowledge no previous pedagogical experiment exists pertaining to the evaluation of immobilization on (observed) enzyme kinetics and substrate mass transfer.…”

Section: Introductionmentioning

confidence: 99%

Simple Protocol to Facilitate Students’ Understanding of the Effects of Enzyme Immobilization on Kinetics of Reaction and Mass Transfer

Simões

Malcata

2020

J. Chem. Educ.

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A laboratory experiment is described in which students carry out immobilization of the enzyme invertase via entrapment in alginate (Ca-alginate) and, accordingly, estimate values for kinetic constants and mass transfer coefficients. Immobilization of enzymes, or confinement thereof to a defined space with retention of most catalytic activity, is attractive for allowing repeated (and continuous) use. However, interest in immobilization hinges critically on whether and how it affects kinetic performance. The overall goal is indeed to retain as much activity as possible and minimize alteration of selectivity, while extending its useful life for as long as feasible. Toward this goal, it is relevant to quantify the effects of immobilization upon enzyme kinetics and mass transfer in particularwith the latter constraining observed (rather than intrinsic) reaction kinetics, owing to diffusional resistance. Invertase was selected as a model enzyme since the associated laboratory handling is safe, fast, reproducible, not expensive, and prone to easily attain the intended learning outcomes.

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“…Although bioconjugation reactions have already been presented in a small number of experimental reports in this Journal , − none of the previous papers mention the application of protein PEGylation in a lab class. As this technique has industrial importance (mainly for the biopharmaceutical sector), the knowledge and application of this reaction in lab classes contributes to a better understanding of biological drugs with chemical modifications by students.…”

Section: Introductionmentioning

confidence: 99%

Tailoring Protein PEGylation Reaction: An Undergraduate Laboratory Experiment

et al. 2020

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Protein PEGylation refers to the covalent binding of polyethylene glycol (PEG) moieties to a protein and has been increasingly applied, mainly in the biopharmaceutical industry. This technique may increase bioavailability and stability by decreasing immunogenicity and proteolytic attack. Given the industrial interest and the relevance of this technique, an example of a common PEGylation reaction is herein proposed, using lysozyme as a model protein. This class is divided into three parts: the first one is composed by the PEGylation reaction per se, the second one aims at the analytical quantification of the PEGylation yield, and the third aims to understand the effects of PEGylation on protein activity. Students should gain skills in comprehending this type of chemical reaction, calculating reaction yields, and realizing the benefits of protein PEGylation. This experimental class comprises two lab classes with the expected duration of 2 h each and is intended for students in the third and fourth years of courses such as pharmaceutical sciences, biochemistry, chemistry, biotechnology, and correlated areas.

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Show Yourself, Asparaginase: An Enzymatic Reaction Explained through a Hands-On Interactive Activity

Cited by 5 publications

References 19 publications

Mapping research on biochemistry education: A bibliometric analysis

Mapping research on biochemistry education: A bibliometric analysis

Simple Protocol to Facilitate Students’ Understanding of the Effects of Enzyme Immobilization on Kinetics of Reaction and Mass Transfer

Tailoring Protein PEGylation Reaction: An Undergraduate Laboratory Experiment

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