Shortening of the development cycle in winter rape (Brassica napus var. oleifera L.) by grafting nonvernalized scions on generative stock

Dubert, F.; Pienkowski, S.; Filek, W.

doi:10.5586/aa.1984.004

Cited by 3 publications

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“…In contrast to other published grafting procedures for B. napus [ 34 – 36 ] this work provides an easy and robust protocol for routine grafting with high success rates. Collar-free grafting is easier to handle and less laborious.…”

Section: Resultsmentioning

confidence: 99%

“…Although not as easy as in Arabidopsis, several reports describe the successful transformation of B. napus using Agrobacterium tumefaciens [ 32 , 33 ] what should allow the creation of suitable transgenic plants for grafting experiments. However, up to now only a few not very detailed grafting protocols for this species have been published in international journals [ 34 – 36 ], and no information about efficiency and reproducibility of grafting is documented.…”

Section: Introductionmentioning

confidence: 99%

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Protocol: optimisation of a grafting protocol for oilseed rape (Brassica napus) for studying long-distance signalling

et al. 2016

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BackgroundGrafting is a well-established technique for studying long-distance transport and signalling processes in higher plants. While oilseed rape has been the subject of comprehensive analyses of xylem and phloem sap to identify macromolecules potentially involved in long-distance information transfer, there is currently no standardised grafting method for this species published.ResultsWe developed a straightforward collar-free grafting protocol for Brassica napus plants with high reproducibility and success rates. Micrografting of seedlings was done on filter paper. Grafting success on different types of regeneration media was measured short-term after grafting and as the long-term survival rate (>14 days) of grafts after the transfer to hydroponic culture or soil.ConclusionsWe compared different methods for grafting B. napus seedlings. Grafting on filter paper with removed cotyledons, a truncated hypocotyl and the addition of low levels of sucrose under long day conditions allowed the highest grafting success. A subsequent long-term hydroponic cultivation of merged grafts showed highest survival rates and best reproducibility.Electronic supplementary materialThe online version of this article (doi:10.1186/s13007-016-0122-x) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protocol: optimisation of a grafting protocol for oilseed rape (Brassica napus) for studying long-distance signalling

et al. 2016

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Observations of Effects of Partial Vernalization in Winter Rape Plants Grafted Onto Generative Stock

Filek

Pienkowski

Dubert

1986

J Agronomy Crop Science

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Winter rape plants vernalized under natural conditions were used as stock for grafting apical and subapical parts of rape seedlings vernalized at 5 °C in light during different periods ranging from 0 to 10 weeks. Acceleration of generative development in both kinds of grafts occurred already after 3 weeks of their vernalization, i.e. after cold treatment of insufficient length to induce flowering in plants developing independently. Effectiveness of vernalization — measured by acceleration of flowering in grafts was highest from 3rd to 5th week of cold treatment and then it declined. Processes occurring during an early stage of vernalization, though insufficient to allow independent generative development, become manifest in grafts made on vernalized stock. This allows investigations of very early stages of vernalization processes inaccessible to observation by other methods.

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Induction of Generative Development of Winter Rape (Brassica napus L. var. oleifera) in Relation to Vernalization Conditions and Age of Vernalized Plants

Dubert

Filek

1994

J Agronomy Crop Science

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Germinating seeds and young winter rape plants were vernalized 56–63 days at 5 or 2°C under nine‐hour days or in darkness. The highest percentage of generative plants and the most rapid flowering were obtained following the vernalization of young seedlings and germinating seed under conditions of nine‐hour day, at 5°C. The least effective induction of generative development followed the vernalization at 2°C in continuous darkness. The vegetation period from the end of vernalization till the beginning of flowering was the shortest when four‐week‐old plants were vernalized under conditions of nine‐hour day, yet the vegetation period from the beginning of germination to flowering was the shortest when seed germinating under conditions of nine‐hour day were vernalized. The period was extending as older plants were being vernalized. Data indicating that the optimal temperature for vernalization of older plants is higher than for germinating seed and young seedlings were obtained.

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Shortening of the development cycle in winter rape (Brassica napus var. oleifera L.) by grafting nonvernalized scions on generative stock

Cited by 3 publications

References 0 publications

Protocol: optimisation of a grafting protocol for oilseed rape (Brassica napus) for studying long-distance signalling

Protocol: optimisation of a grafting protocol for oilseed rape (Brassica napus) for studying long-distance signalling

Observations of Effects of Partial Vernalization in Winter Rape Plants Grafted Onto Generative Stock

Induction of Generative Development of Winter Rape (Brassica napus L. var. oleifera) in Relation to Vernalization Conditions and Age of Vernalized Plants

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