Shortening of luteal phase in common marmosets by sheep ovarian follicular fluid peptide

Effect of Ovarian Follicular Fluid Peptide (OFFP) on FSH and EGF Binding to Granulosa Cells

“…The purification of sheep ovarian follicular fluid peptide has been described in detail earlier (Nandedkar, Kadam and Moodbidri 1988). Briefly, sheep follicular fluid was purified by gel permeation chromatography on Sephadex G-100 column followed by Sephadex G-25 column using 1 % ammonium bicarbonate as the eluting buffer.…”

“…An ovarian follicular fluid peptide (OFFP) has been partially purified by us from sheep ovaries which inhibits ovulation in normal cycling mice (Nandedkar, Kadam and Moodbidri 1988). Furthermore, it shortens the luteal phase and impairs fertility in marmosets (Nandedkar, Kholkute, Kadam, Moodbidri and Raghavan 1989a;Nandedkar, Kholkute, Shahid and Moodbidri 1989b). Preliminary studies indicate that the peptide inhibits FSH binding to granulosa cells (Nandedkar et al 1989a).…”

“…Furthermore, it shortens the luteal phase and impairs fertility in marmosets (Nandedkar, Kholkute, Kadam, Moodbidri and Raghavan 1989a;Nandedkar, Kholkute, Shahid and Moodbidri 1989b). Preliminary studies indicate that the peptide inhibits FSH binding to granulosa cells (Nandedkar et al 1989a).…”