Luteal deficiency caused by human ovarian follicular fluid peptide in post-partum marmosets,

Effect of the human follicle-stimulating hormone-binding inhibitor and its N-terminal fragment on follicle-stimulating hormone-induced progesterone secretion by granulosa cells in vitro

“…Earlier studies have shown that treatment of female marmosets with the OP led to luteal insuffi ciency and induced abortion in these animals by lowering P 4 levels during the luteal phase (Nandedkar et al 1989;Wadia et al 2003). The effect of the OP on P 4 secretion was due to its ability to prevent the action of FSH on the follicles during the follicular phase of the ovarian cycle resulting in luteal insuffi ciency and premature termination of pregnancy.…”

“…We have previously reported (Nandedkar et al 1989(Nandedkar et al , 2001 that human ovarian follicular fl uid contains a low molecularweight protein termed FSHBI, which inhibits binding of FSH to its receptors on granulosa cells. A synthetic peptide corresponding to the N-terminal region (OP) of human FSHBI also demonstrated FSHBI activity similar to the native protein.…”

“…Our laboratory has identifi ed one such low molecularweight peptide (<5 kDa) from sheep and human ovarian follicular fl uid termed ovarian follicular fl uid peptide. This peptide blocks binding of 125 I-hFSH to receptors on granulosa cells in a radioreceptor assay (Nandedkar et al 1989) and preliminary studies performed using the partially purifi ed form of the peptide of human origin have indicated that it is secreted by granulosa cells of medium and atretic follicles (Nandedkar et al 1994). Administration of the human ovarian follicular fl uid peptide, now referred to as FSH-binding inhibitor (FSHBI) and fi rst identifi ed by Darga and Reichert (1978), suppresses ovulation and induces follicular atresia in adult mice (Nandedkar et al 1988;.…”

“…Administration of the human ovarian follicular fl uid peptide, now referred to as FSH-binding inhibitor (FSHBI) and fi rst identifi ed by Darga and Reichert (1978), suppresses ovulation and induces follicular atresia in adult mice (Nandedkar et al 1988;. When administered during the preovulatory period in the common marmoset, the peptide induced luteal insuffi ciency and impaired fertility (Nandedkar et al 1989). Our laboratory has purifi ed FSHBI from human ovarian follicular fl uid (Nandedkar et al 2001;Wadia et al 2003).…”

Effect of Ovarian Follicular Fluid Peptide (OFFP) on FSH and EGF Binding to Granulosa Cells

“…An ovarian follicular fluid peptide (OFFP) has been partially purified by us from sheep ovaries which inhibits ovulation in normal cycling mice (Nandedkar, Kadam and Moodbidri 1988). Furthermore, it shortens the luteal phase and impairs fertility in marmosets (Nandedkar, Kholkute, Kadam, Moodbidri and Raghavan 1989a;Nandedkar, Kholkute, Shahid and Moodbidri 1989b). Preliminary studies indicate that the peptide inhibits FSH binding to granulosa cells (Nandedkar et al 1989a).…”