Short-Term Pulmonary Response to Inhaled JP-8 Jet Fuel Aerosol in Mice

Robledo, Raymond F.; Young, Renée; Lantz, R. Clark; Witten, Mark L.

doi:10.1177/019262330002800504

Cited by 48 publications

(41 citation statements)

References 15 publications

(16 reference statements)

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“…A typical morphologic change associated with impaired barrier function is perivascular edema which was observed in our in vivo morphological evaluations (19). In addition, ultrastructural evaluation of alveolar type II cells revealed an apparent increase in the number and size of surfactant secreting lamellar bodies (19).…”

Section: Morphological Evaluation-histology and Micrographymentioning

confidence: 61%

“…In this previous mouse in vivo model, morphological evaluation following acute JP-8 exposures revealed lung lesions following doses of 50 mg/m 3 and 113 mg/m 3 (19). Specifically, alveolar injury, as shown by light microscopy sections embedded in paraffin and stained with hematoxylin and eosin, was characterized by the appearance of nondiffuse alveolar-septal thickening.…”

Section: Introductionmentioning

confidence: 86%

“…Morphological evaluation after acute JP-8 aerosol exposures showed lesions following a range of doses up to 113 mg/m 3 (4,17,19). Subsequent in vitro experiments in our labs explored the role of epithelial barrier function upon JP-8 exposure.…”

Section: Morphological Evaluation-histology and Micrographymentioning

confidence: 99%

“…The objective of this report is to demonstrate that the site(s) of injury in precision-cut lung slices exposed in vitro to JP-8 is similar to lesions observed following in vivo exposure to jet fuel (19). Demonstration that the cellular targets of in vitro exposure are similar to in vivo targets would allow us to utilize the in vitro slice model to study the mechanism of JP-8 jet fuel-induced lung injury.…”

Section: Introductionmentioning

confidence: 95%

“…Specifically, alveolar injury, as shown by light microscopy sections embedded in paraffin and stained with hematoxylin and eosin, was characterized by the appearance of nondiffuse alveolar-septal thickening. In addition, ultrastructural evaluation of alveolar type II cells, as shown by electron microscopy, revealed an apparent increase in the number and size of surfactant secreting lamellar bodies (19).…”

Section: Introductionmentioning

confidence: 95%

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Correlation Between In Vivo and In Vitro Pulmonary Responses to Jet Propulsion Fuel-8 Using Precision-Cut Lung Slices and a Dynamic Organ Culture System

2003

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In tissue slice models, interactions between the heterogeneous cell types comprising the lung parenchyma are maintained thus providing a controlled system for the study of pulmonary toxicology in vitro. However, validation of the model in vitro system must be affirmed. Previous reports, in in vivo systems, have demonstrated that Clara cells and alveolar type II cells are the targets following inhalation of JP-8 jet fuel. We have utilized the lung slice model to determine if cellular targets are similar following in vitro exposure to JP-8. Agar-filled adult rat lung explants were cored and precision cut, using the Brendel/Vitron tissue slicer. Slices were cultured on titanium screens located as half-cylinders in cylindrical Teflon cradles that were loaded into standard scintillation vials and incubated at 37 • C. Slices were exposed to JP-8 jet fuel (0.5 mg/ml, 1.0 mg/ml, and 1.5 mg/ml in medium) for up to 24 hours. We determined ATP content using a luciferin-luciferase bioluminescent assay. No significant difference was found between the JP-8 jet fuel doses or time points, when compared to controls. Results were correlated with structural alterations following aerosol inhalation of JP-8. As a general observation, ultrastructural evaluation of alveolar type II cells revealed an apparent increase in the number and size of surfactant secreting lamellar bodies that was JP-8 jet fuel-dose dependent. These results are similar to those observed following aerosol inhalation exposure. Thus, the lung tissue slice model appears to mimic in vivo effects of JP-8 and therefore is a useful model system for studying the mechanisms of lung injury following JP-8 exposure.

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Section: Morphological Evaluation-histology and Micrographymentioning

confidence: 61%

Section: Introductionmentioning

confidence: 86%

Section: Morphological Evaluation-histology and Micrographymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 95%

See 3 more Smart Citations

Correlation Between In Vivo and In Vitro Pulmonary Responses to Jet Propulsion Fuel-8 Using Precision-Cut Lung Slices and a Dynamic Organ Culture System

2003

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Acute respiratory distress associated with inhaled hydrocarbon

Weibrecht

Rhyee

2011

Am. J. Ind. Med.

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Detection of oxidative species and low‐molecular‐weight DNA in skin following dermal exposure with JP‐8 jet fuel

Rogers¹,

Gunasekar²,

Garrett³

et al. 2001

J of Applied Toxicology

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Dermal absorption of JP-8 jet fuel can lead to skin irritation within hours after exposure. This study detected the formation of oxidative species and low-molecular-weight DNA in rat skin as potential indicators of JP-8-induced skin injury. At 0, 1, 2, 4 and 6 h after the beginning of a 1-h exposure, skin samples were removed and analyzed for oxidative species formation and low-molecular-weight DNA analysis. At 1, 2 and 4 h, mean oxidative species levels increased significantly (P < 0.05) above unexposed samples. Significantly higher (P < 0.05) low-molecular-weight DNA values were observed at 4 and 6 h compared with unexposed controls. These results demonstrate significant increases in oxidative species and low-molecular-weight DNA levels in the skin following dermal exposure to JP-8. These responses may serve as indicators of skin injury following exposure to JP-8 jet fuel and other volatile chemicals or mixtures.

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Short-Term Pulmonary Response to Inhaled JP-8 Jet Fuel Aerosol in Mice

Cited by 48 publications

References 15 publications

Correlation Between In Vivo and In Vitro Pulmonary Responses to Jet Propulsion Fuel-8 Using Precision-Cut Lung Slices and a Dynamic Organ Culture System

Correlation Between In Vivo and In Vitro Pulmonary Responses to Jet Propulsion Fuel-8 Using Precision-Cut Lung Slices and a Dynamic Organ Culture System

Acute respiratory distress associated with inhaled hydrocarbon

Detection of oxidative species and low‐molecular‐weight DNA in skin following dermal exposure with JP‐8 jet fuel

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