Short-term induction of vitellogenesis in the immature male yellowfin seabream (<i>Acanthopagrus latus</i>) exposed to bisphenol A and 17<i>β</i>-estradiol

Negintaji, Ahmad; Safahieh, Alireza; Zolgharnein, Hossein; Matroodi, Soheila

doi:10.1177/0748233717748099

Cited by 18 publications

(9 citation statements)

References 61 publications

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“…Sparidae is a group of teleost fish with different reproductive strategies, including gonochorism and hermaphroditism. For example, the common dentex (Dentex dentex) is a gonochoristic species (Tsakogiannis et al, 2018a), red porgy (Pagrus pagrus), and common pandora (pagellus erythrinus) are protogynous hermaphrodites (Tsakogiannis et al, 2018b), gilthead seabream (Sparus aurata), and yellowfin seabream (Acanthopagrus latus) are protandrous hermaphrodites (Negintaji et al, 2018;Tsakogiannis et al, 2018a), and sharpsnout seabream (Diplodus puntazzo) is a rudimentary hermaphrodite fish species (Papadaki et al, 2018). Therefore, Sparidae is a good model for studying SD and sex reversal.…”

Section: Introductionmentioning

confidence: 99%

Gonadal Transcriptome Analysis of Sex-Related Genes in the Protandrous Yellowfin Seabream (Acanthopagrus latus)

Lin

Fang

et al. 2020

Front. Genet.

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Yellowfin seabream (Acanthopagrus latus), a protandrous hermaphroditic fish, is a good model for studying the mechanism of sex reversal. However, limited knowledge is known about the genetic information related to reproduction and sex differentiation in this species. Here, we performed de novo transcriptome sequencing analysis of the testis, ovotestis, and ovary to identify sex-related genes in yellowfin seabream. The results assembled 71,765 unigenes in which 16,126 and 17,560 unigenes were differentially expressed in the ovotestis and ovary compared to the testis, respectively. The most differentially expressed gene (DEG)-enriched Kyoto Encyclopedia of Genes and Genomes and GO pathways were closely associated with the synthesis of sex steroid hormones. Functional analyses identified 55 important sex-related DEGs, including 32 testis-biased DEGs (dmrt1, amh, and sox9, etc.), 20 ovary-biased DEGs (cyp19a, foxl2, and wnt4, etc.), and 3 ovotestis-biased DEGs (lhb, dmrt2, and foxh1). Furthermore, the testis-specific expression of dmrt1 and the brain-pituitary-ovary axis expression of foxl2 were characterized, suggesting that they might play important roles in sex differentiation in yellowfin seabream. Our present work provided an important molecular basis for elucidating the mechanisms underlying sexual transition and reproductional regulation in yellowfin seabream.

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Section: Introductionmentioning

confidence: 99%

Gonadal Transcriptome Analysis of Sex-Related Genes in the Protandrous Yellowfin Seabream (Acanthopagrus latus)

Lin

Fang

et al. 2020

Front. Genet.

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“…Environmental degradation by anthropic activities strongly affects ecosystems, mainly due to exposure to various contaminants (Solaun et al 2021). Even when present in concentrations in the order of μg L -1 and ng L -1 , several substances can cause risks to both human and animal health (Liu et al 2018;Negintaji et al 2018). Concerns regarding the quality of water resources are extremely relevant, due to significant increases in emerging micropollutants, as well as their by-products and metabolites originating from the chemical and biological degradation of the original compounds (Locatelli et al 2016;Zhong et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Estrogenic Activity and Endocrine Disruptor Compounds Determined in Guanabara Bay (Brazil) by Yeast Estrogen Screen Assays and Chemical Analyses

Nascimento¹,

Santos²,

Cunha³

et al. 2022

Anu. Inst. Geociênc.

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Studies assessing the presence of endocrine disrupting compounds in marine environments have increased in the last decades. In Brazil, the combination of poor sanitation conditions and low investment in sewage treatment plants leads to significant contamination of receiving waters. The risks of these micropollutants in the aquatic biota include biochemical and histopathological alterations of the liver, gonads, and kidneys, as well as, reproductive process and development modifications, and behavioral changes, among others. The aim of this study was to evaluate the quality of the surface and deep waters of Guanabara Bay, southeastern Brazil, regarding the presence of estrogenic substances. Acute toxicity assays were also conducted employing Vibrio fischeri. The estrogenic activity of the water samples was determined by Yeast Estrogen Screen assay and the quantification of the Bisphenol A, estriol, 17β-estradiol, and 17α-ethinylestradiol by high-performance liquid chromatography, using fluorescence and diode array detectors. Estrogenic activity ranged from 9 to 77 ng L-1 of estradiol equivalents. The highest micropollutants concentrations were detected for bisphenol A (298.5 and 465.5 ng L-1), followed by 17α-ethinylestradiol (248 and 256.9 ng L-1), estriol (70.7 and 179.6 ng L-1), and 17β-estradiol (167 and 174.8 ng L-1) for surface and deep waters, respectively. The findings indicate significant risks for the Guanabara Bay ecosystem. No acute toxicity effects were observed in the V. fisheri assay. These data reflect the current environmental degradation situation of the bay’s waters and highlight the need for the systematic monitoring of this important estuary.

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“…The use of qPCR assay for VTG mRNA in several teleosts has been developed in other countries (Ciu et al 2017;Mushirobira et al 2020) and they are used as the basis for policy formulation and monitoring aquatic environments. In previous studies, optimization of the qPCR assay is done using sexually mature females (Bowley et al 2010) or hormonally induced males (Caspillo et al 2014;Negintaji et al 2018) to ensure the presence of VTGs and then characterize the isolated VTG, and sequenced. Some fish species used in VTG quantification studies were zebrafish, Danio rerio (Humble et al 2013;Chen et al 2018), tilapia, Oreochromis mossambicus (Celino-Brady et al 2019;Chen et al 2020), These studies showed that the presence of VTG in male fish is a good biomarker for contamination of estrogens.…”

Section: Introductionmentioning

confidence: 99%

Identification and quantification of vitellogenin gene in eyebrow goby (Oxyurichthys ophthalmonema)

et al. 2022

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Abstract. Sa-An AC, Quinitio GF, Anasco NC, Traifalgar RFM, Nillos MGG. 2022. Identification and quantification of vitellogenin gene in eyebrow goby (Oxyurichthys ophthalmonema). Biodiversitas 23: 5606-5613. Vitellogenin (VTG) gene has been used as a biomarker for exposure to estrogenic compounds in marine and freshwater fish species. There was limited information about the detection of VTG mRNA in an estuarine environment using a bioindicator fish species. In this study, a quantitative real-time polymerase chain reaction (qPCR) assay was developed for quantification of VTG mRNA normalized to ?-actin in eyebrow goby, Oxyurichthys ophthalmonema. VTG mRNA in the liver of reproductively mature female samples was isolated, amplified, and sequenced. There were 430 bp fragments of VTG, corresponding to a 143 amino acid sequence obtained from eyebrow goby. The partial protein sequence exhibited similar identities with the phosvitin motif of VTG in other gobies. The new qPCR assay was developed through the evaluation of hepatic mRNA levels in nonreproductive male eyebrow goby at 0, 6, 12, and 24 h following intraperitoneal injection of 10 mg kg-1 17?-estradiol. Hepatic mRNA levels were detected 6 h after 17?-estradiol injection and the highest VTG expression was noted 24 h after injection. Results of the present study suggest that male eyebrow goby can be used as indicator species for estrogenic contamination. Likewise, the established qPCR assay can be used to assess recent exposure to estrogenic contaminants in the aquatic environment. The characterization of the amplified VTG gene from eyebrow goby and the application of the qPCR assay developed for eyebrow goby in monitoring EDCs in the field may be considered for future investigation.

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Short-term induction of vitellogenesis in the immature male yellowfin seabream (Acanthopagrus latus) exposed to bisphenol A and 17β-estradiol

Cited by 18 publications

References 61 publications

Gonadal Transcriptome Analysis of Sex-Related Genes in the Protandrous Yellowfin Seabream (Acanthopagrus latus)

Gonadal Transcriptome Analysis of Sex-Related Genes in the Protandrous Yellowfin Seabream (Acanthopagrus latus)

Estrogenic Activity and Endocrine Disruptor Compounds Determined in Guanabara Bay (Brazil) by Yeast Estrogen Screen Assays and Chemical Analyses

Identification and quantification of vitellogenin gene in eyebrow goby (Oxyurichthys ophthalmonema)

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