Short-term effects of extremely low-frequency pulsed electromagnetic field and pulsed low-level laser therapy on rabbit model of corneal alkali burn

Kanavi, Mozhgan Rezaei; Tabeie, Faraj; Sahebjam, Farzin; Poursani, Nima; Jahanbakhsh, Nazanin; Paymanpour, Pouya; Afsar-Aski, Sasha

doi:10.1016/j.exer.2016.01.007

Cited by 16 publications

(8 citation statements)

References 36 publications

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“…Clinical characterization for LSCD includes corneal opacity, persist epithelial defects, neovascularization and high goblet cell density in cornea 11 , 12 , 26 . In our rabbit model, alkali burn without treatment (control group) developed severe cornea opacity, neovascularization, and conjunctivalization of the corneal surface within two weeks.…”

Section: Resultsmentioning

confidence: 99%

Human limbal niche cells are a powerful regenerative source for the prevention of limbal stem cell deficiency in a rabbit model

Zhang²,

Cai

et al. 2018

Sci Rep

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In this article, human limbal niche cells (LNC) or bone marrow derived mesenchymal stem cells (BMMSC) were used to prevent limbal stem cell deficiency (LSCD) in an alkali burn rabbit model and their results were compared. The epithelial cell defect area, corneal neovascularization, and the print cell cytometry were quantified to grade the severity of LSCD. Three months after the alkali burn, a partial LSCD was observed in the control group (no treatment) indicated by chronic corneal epithelial defects, positive corneal fluorescein staining, neovascularization and goblet cell migration. In contrast, the severity of LSCD in both the LNC and BMMSC transplantation groups was dramatically reduced as shown by smaller epithelial cell defects, decreased fluorescein sodium staining, decreased neovascularization and decreased goblet cell density. Interestingly, the LNC group was shown to more effectively prevent LSCD than the BMMSC group. Further analysis indicated subconjunctivally transplanted LNCs were more powerful than BMMSCs to prevent LSCD, at least partially, due to increased activation of SCF-c-Kit signal. We conclude that LNCs are a more powerful resource than BMMSCs to prevent LSCD in an alkali burn rabbit model, at least partially due to increased activation of SCF signaling.

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Section: Resultsmentioning

confidence: 99%

Human limbal niche cells are a powerful regenerative source for the prevention of limbal stem cell deficiency in a rabbit model

Zhang²,

Cai

et al. 2018

Sci Rep

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“…1 ). Clinical observation showed that alkali burn leads to corneal opacity, epithelial defects, and neovascularization of the cornea 23 - 25 . SJS involved ocular surfaces were also very similar 26 .…”

Section: Resultsmentioning

confidence: 99%

“…LSCD can arise from diseases such as Stevens Johnson Syndrome and through injuries such as alkali burn. Characteristics of LSCD include corneal epithelial defects, neovascularization, ingrowth of the conjunctival epithelium, and chronic inflammation 23 - 25 .These events eventually lead to visual loss 12 , 35 , 36 . Traditionally, treatment of LSCD patients is limbal tissue transplantation, which is obtained from a fellow healthy eye or a donor eye 37 .…”

Section: Discussionmentioning

confidence: 99%

A New Isolation Method of Human Lacrimal Canaliculus Epithelial Stem Cells by Maintaining Close Association with Their Niche Cells

Zhang

Tighe

et al. 2018

Int. J. Med. Sci.

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Purpose: To investigate whether lacrimal canaliculus epithelial stem cells (LCESC) could be isolated and expanded in vitro.Methods: The lacrimal canaliculus epithelium of 6 patients with limbal stem cell deficiency (LSCD) caused by alkali burn or Stevens Johnson Syndrome were examined by lacrimal endoscope. Cadaveric eyelids were fixed and prepared for cross section and stained with HE and antibodies against PCK, Vim, p63α, SCF and c-Kit. Canaliculus tissue was separated under an operating microscope using a lacrimal probe as an indicator and digested with collagenase A. The clusters of epithelial cells with closely associated stroma were further digested with Trypsin/EDTA to obtain single cells for culture on Matrigel-coated plastic plates in MESCM media. The expression of SCF, c-Kit and p63α was determined by immunostaining. The colony-forming efficiency on 3T3 feeder layers was also measured by calculating the percentage of the clone number divided by the total number cells seeded.Results: The epithelial layers of five out of six inferior lacrimal canaliculi and all the six superior lacrimal canaliculi were visually normal in appearance. Five to fifteen layers of the epithelium in the human lacrimal canaliculi were present with a small, tightly compacted basal layer of cells expressing PCK, p63α, SCF and c-Kit. LCESC were isolated by collagenase A and obtained clonal growth in MESCM. The colony-forming efficiency of LCESC holoclones on a 3T3 feeder layer was 3.2%, compared to 1.9% for those of limbal stem cells (LSC).Conclusions: Herein, we first report that LCESCs can be isolated and have stem cell characteristics, similar to those of LSCs. Such a discovery raises a promising substrate resource of stem cells for LSC reconstruction in LSCD patients.

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“…Depending on the intensity, exposure time, and cell types studied, heterogeneous effects of EMFs have been reported. For instance, in a series of experimental studies, short‐term application of EMFs of 2 mT‐intensity and frequency of 25 Hz was reported as a successful treatment modality for corneal wound healing [Rezaei et al, , ]. However, prolonged exposure to low‐frequency pulsed electromagnetic field (PEMF) has been considered a carcinogen, and DNA damage and alteration of cells to become cancerous have been reported from the effects of 50 to 60 Hz fields [McLean et al, ; Lai and Singh, ; Ivancsits et al, ; Wolf et al, ].…”

Section: Introductionmentioning

confidence: 99%

The effects of electromagnetic fields on cultured human retinal pigment epithelial cells

Nasrabadi

Soheili

Bagheri

et al. 2018

Bioelectromagnetics

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A great deal of evidence has confirmed that electromagnetic fields (EMFs) can affect the central nervous system. In this study, cultured neonatal human retinal pigment epithelial (hRPE) cells were exposed to pulsed EMF of 1 mT intensity and 50 Hz frequency 8 h daily for 3 days. In addition to cell proliferation and cell death assays, immunocytochemistry for RPE65, PAX6, nestin, and cytokeratin 8/18 proteins were performed. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) was performed for NES, PAX6, RPE65, and ACTA2 gene expression. Exposed hRPE cells did not demonstrate significant change in terms of cytomorphology, cell proliferation, or cell death. Protein expression of PAX6 was decreased in treated cells compared to controls and remained unchanged for RPE65, cytokeratin 8/18, and nestin. Gene expressions of NES, RPE65, and PAX6 were decreased in treated cells as compared to controls. Gene expression of ACTA2 did not significantly change. In conclusion, viability of cultivated neonatal hRPE cells did not change after short exposure to a safe dose of pulsed EMF albeit that both gene and protein expressions of retinal progenitor cell markers were reduced. Whether longer exposure durations that are being constantly produced by widely-used electronic devices may induce significant changes in these cells, needs further investigation. Bioelectromagnetics. 39:585-594, 2018.

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Short-term effects of extremely low-frequency pulsed electromagnetic field and pulsed low-level laser therapy on rabbit model of corneal alkali burn

Cited by 16 publications

References 36 publications

Human limbal niche cells are a powerful regenerative source for the prevention of limbal stem cell deficiency in a rabbit model

Human limbal niche cells are a powerful regenerative source for the prevention of limbal stem cell deficiency in a rabbit model

A New Isolation Method of Human Lacrimal Canaliculus Epithelial Stem Cells by Maintaining Close Association with Their Niche Cells

The effects of electromagnetic fields on cultured human retinal pigment epithelial cells

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