Short-Term Effects of Extremely Low Frequency Pulsed Electromagnetic Field on Corneas with Alkaline Burns in Rabbits

Kanavi, Mozhgan Rezaei; Sahebjam, Farzin; Tabeie, Faraj; Davari, Paniz; Samadian, Aminpasha; Yaseri, Mehdi

doi:10.1167/iovs.12-10248

Cited by 7 publications

(4 citation statements)

References 31 publications

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“…These stresses seem to greatest in the central cornea and contribute to the preferential exfoliation of cells from the epithelium . An alternative, but not mutually exclusive theory is that electromagnetic fields of the eye may influence centripetal migration, and this has been described as a vortex pattern of cell movement . Other studies have shown that sympathetic nerves stimulate epithelial proliferation during both homeostasis and wound‐healing and may also influence cell migration .…”

Section: Discussionmentioning

confidence: 99%

Tracing the Fate of Limbal Epithelial Progenitor Cells in the Murine Cornea

et al. 2014

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Stem cell (SC) division, deployment, and differentiation are processes that contribute to corneal epithelial renewal. Until now studying the destiny of these cells in a living mammal has not been possible. However, the advent of inducible multicolor genetic tagging and powerful imaging technologies has rendered this achievable in the translucent and readily accessible murine cornea. K14CreERT2‐Confetti mice that harbor two copies of the Brainbow 2.1 cassette, yielding up to 10 colors from the stochastic recombination of fluorescent proteins, were used to monitor K‐14+ progenitor cell dynamics within the corneal epithelium in live animals. Multicolored columns of cells emerged from the basal limbal epithelium as they expanded and migrated linearly at a rate of 10.8 µm/day toward the central cornea. Moreover, the permanent expression of fluorophores, passed on from progenitor to progeny, assisted in discriminating individual clones as spectrally distinct streaks containing more than 1,000 cells within the illuminated area. The centripetal clonal expansion is suggestive that a single progenitor cell is responsible for maintaining a narrow corridor of corneal epithelial cells. Our data are in agreement with the limbus as the repository for SC as opposed to SC being distributed throughout the central cornea. This is the first report describing stem/progenitor cell fate determination in the murine cornea using multicolor genetic tracing. This model represents a powerful new resource to monitor SC kinetics and fate choice under homeostatic conditions, and may assist in assessing clonal evolution during corneal development, aging, wound‐healing, disease, and following transplantation. Stem Cells 2015;33:157–169

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Section: Discussionmentioning

confidence: 99%

Tracing the Fate of Limbal Epithelial Progenitor Cells in the Murine Cornea

et al. 2014

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“…In contrast, control groups (RS and RB) had irregular regenerating epithelium with uneven thickness, significant stromal keratocyte loss, and undesirable architecture distortion. In addition, compared to the reported nonpharmacological electromagnetic and laser therapeutic approaches based on similar rabbit models (14-day treatment course) 52 , 53 (Fig. 4h ), our correlated BPCL system demonstrated outstanding comprehensive repair effect under the evaluation criteria of the corneal clarity recovery, epithelial integrity and thickness, stromal keratocyte loss, and endothelium integrity.…”

Section: Resultsmentioning

confidence: 58%

Snowflake-inspired and blink-driven flexible piezoelectric contact lenses for effective corneal injury repair

Yao,

Mo,

Liu

et al. 2023

Nat Commun

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The cornea is a tissue susceptible to various injuries and traumas with a complicated cascade repair process, in which conserving its integrity and clarity is critical to restoring visual function. Enhancing the endogenous electric field is recognized as an effective method of accelerating corneal injury repair. However, current equipment limitations and implementation complexities hinder its widespread adoption. Here, we propose a snowflake-inspired, blink-driven flexible piezoelectric contact lens that can convert mechanical blink motions into a unidirectional pulsed electric field for direct application to moderate corneal injury repair. The device is validated on mouse and rabbit models with different relative corneal alkali burn ratios to modulate the microenvironment, alleviate stromal fibrosis, promote orderly epithelial arrangement and differentiation, and restore corneal clarity. Within an 8-day intervention, the corneal clarity of mice and rabbits improves by more than 50%, and the repair rate of mouse and rabbit corneas increases by over 52%. Mechanistically, the device intervention is advantageous in blocking growth factors’ signaling pathways specifically involved in stromal fibrosis whilst preserving and harnessing the signaling pathways required for indispensable epithelial metabolism. This work put forward an efficient and orderly corneal therapeutic technology utilizing artificial endogenous-strengthened signals generated by spontaneous body activities.

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“…In particular, experiences both in animal models and in clinical settings have confirmed the safety and effectiveness of TMR in promoting tissue repair in posttraumatic and chronic wounds, making this technology potentially interesting for the management of DF postsurgical wounds, 12,13 as a recent clinical experience reported. 14…”

mentioning

confidence: 93%

Safety and Effectiveness of Therapeutic Magnetic Resonance in the Management of Postsurgical Lesion of the Diabetic Foot

Abbruzzese

Iacopi

Coppelli

et al. 2015

The International Journal of Lower Extremity Wounds

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To evaluate the safety and effectiveness of therapeutic magnetic resonance (TMR) in the management of the diabetic foot (DF), we treated a group of consecutive type 2 diabetic inpatients with wide postsurgical lesions (Group A: N = 10; age 67.7 ± 18.9 years, duration of diabetes 22.3 ± 6.6 years, 8.1 ± 1.1%, body mass index 29.4 ± 2.1 kg/m(2)), for 2 consecutive weeks, while admitted, with a low-intensity magnetic resonance equipment, in addition to standard treatment. Patients, compared with a matched control group with the same clinical characteristics (Group B), were then followed monthly for 6 months to evaluate healing rate (HR), healing time (HT), rate of granulation tissue (GT) at 3 months, and adverse events. HR was of 90% in Group A and 30% in Group B (P < .05); GT was 73.7 ± 13.2% in Group A versus 51.84 ± 18.77% in Group B (P < .05). HT in Group A was 84.46 ± 54.38 days versus 148.54 ± 78.96 days in Group B (P < .01). No difference in adverse events (5 in Group A and 6 in Group B) was observed throughout the study period. In this pilot study, the use of TMR at this dose and duration was safe. The results also permit the observation that TMR plus standard care offered a faster healing rate compared with standard care alone.

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Short-Term Effects of Extremely Low Frequency Pulsed Electromagnetic Field on Corneas with Alkaline Burns in Rabbits

Cited by 7 publications

References 31 publications

Tracing the Fate of Limbal Epithelial Progenitor Cells in the Murine Cornea

Tracing the Fate of Limbal Epithelial Progenitor Cells in the Murine Cornea

Snowflake-inspired and blink-driven flexible piezoelectric contact lenses for effective corneal injury repair

Safety and Effectiveness of Therapeutic Magnetic Resonance in the Management of Postsurgical Lesion of the Diabetic Foot

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