Short Term Effect of He-Ne Laser on Jatropha curcas Leaf Development and Amino Acid Biosynthesis: More Insights in Developmental Genetics of Oil Producing Plants

Abstract

JATROPHA curcas is a promising plant for biodiesel production. One tentative way to increase oil production is using non traditional physical methods such as laser. It had been shown before that laser could enhance plant growth and development as general. In this study, the DNA was extracted from leaves of plants grown from seeds collected from different regions in Egypt and tested using PCR to confirm their homogenousity. Then, the fully expanded leaves of Jatropha (6-week-old plants) were exposed to a dose equaling 300 mJ cm -2 Helium-Neon (He-Ne) laser. The effect of the administered dose on the photosynthetic pigment expression, chloroplast biogenesis and total amino acid biosynthesis were evaluated after 0, 1 and 24 h post irradiation. Laser treatment in this work did enhance all the previousely mentioned parametrs as will be discussed further in relation to the possibility of using laser as a novel protocol to enhance field crop plants as general.

Effect of Long-Term of He-Ne Laser Light Irradiation on Selected Physiological Processes of Triticale

“…The increase in the metabolic activity of seeds is due to the absorption of more energy from the environment [12]. As indicated by the results of Khalifa et al [18], the transformation of glutamate to proline may refer to the induction of proline biosynthesis in plants by increasing the thickness of the cell walls. Irradiation of seeds with laser light stimulates ATP production, and thus an increase in crops in the form of mass [17,35].…”

“…Photosynthesis is directly related to the production of biomass and the crop, as it is the source of energy for carbon fixation [40][41][42]. The laser light influences the metabolic activity of chloroplasts, leading, for example, to an increase in protein content through increased amino acid assimilation and increased starch storage [18,38]. According to Perveen et al [38] and Chen et al [12], pre-sowing laser light treatment of seeds increases the chlorophyll concentration.…”

“…Previous experiments on irradiation with laser light focused on short-term, several seconds long, time of irradiation of seeds [12,[16][17][18]. In this experiment, an attempt was made to investigate the effect of pre-sowing light irradiation of triticale grains with He-Ne laser in two-time intervals (3 and 24 h) on the germination and further growth of seedlings.…”

Effect of Laser Irradiation and Rhizobium on Growth Parameters of Clover

“…However, information regarding the myco ora on soybean seeds coupled with laser irradiation is scarce. Khalifa et al [2017], indicated that the transformation of glutamate to proline may refer to the induction of proline biosynthesis in plants by increasing the thickness of the cell walls. Irradiation of seeds with laser light stimulates ATP production, and thus an increase in crops in the form of mass [Wilczek et al, 2006].…”