Short-Term Collagen Nerve Wrapping Facilitates Motor and Sensory Recovery from Nerve Degeneration in a Sciatic Nerve Injury Rat Model

Abstract

Purpose This study used a sciatic nerve injury rat model to investigate the short-term effects of a polyglycolic acid (PGA)-collagen tube for nerve injury in continuity. Materials and Methods Sixteen female Wistar rats (6–8 weeks) were used, and the left sciatic nerve was crushed with a Sugita aneurysm clip. Sciatic nerve model rats were randomly categorized into two groups (n = 8; control group, n = 8; nerve wrapping group). Then, we measured four sensory thresholds, magnetically stimulated the lumbar region to induce motor-evoked potentials (MEPs), and evaluated the sciatic nerve histopathologically. Results In the sensory thresholds, there were significant differences for the main effect in 250 and 2000 Hz stimulation (p = 0.048 and 0.006, respectively). Further, a significant difference was observed with 2000 Hz stimulation at 1 week (p = 0.003). In the heat stimulation, there were significant differences for the main effect in both weeks and groups (p = 0.0002 and 0.0185, respectively). The post-hoc test showed a significant difference between groups only in 2W (p = 0.0283). Three weeks after the surgery, both 2nd and 3rd MEPs waves-related latencies in the nerve wrapping group were significantly shorter than those in the control group (p = 0.0207 and 0.0271, respectively). Histological evaluation of the sciatic nerve revealed considerable differences in the number of axons between the two groups (p = 0.0352). Conclusion The short-term PGA-collagen tube nerve wrapping facilitated motor and sensory recovery from nerve degeneration in the sciatic nerve injury rat model.

Whole body vibration accelerates the functional recovery of motor nerve components in sciatic nerve-crush injury model rats

“…1Bi ). The first was stable and symmetrical ( Sonohata et al, 2023 ), the second was intensity dependency for the LMS, and the third was composed of 1st negative wave, 2nd positive wave, and 3rd small negative ( Fig. 1Bi ) ( Sonohata et al, 2023 ).…”

“…The Intercross-210 was connected to a personal computer. We measured the time from the onset of heat stimulation to the observation of the withdrawal reflex ( Sonohata et al, 2023 ).…”

“…A single magnetic stimulation was imposed on the rats, and MEPs were recorded into lab chart 8 software via power lab system (AD Instruments, Dunedin, New Zealand). We used magnetic stimulation on the lumbar region (LMS)-induced MEPs to measure motor nerve responses ( Sonohata et al, 2023 ). To simultaneously record bilateral responses from both gastrocnemius muscles, two- needle electrodes for non-references (MLA1203, needle electrode, AD Instruments) were inserted into the middle portion of the lateral head at the bilateral gastrocnemius.…”

“…Two ground electrodes (EM-275S, Noraxon) were put on the bilateral pelvic area. The low-cut filter for the recording was 300 Hz, and the high-cut was 500 Hz ( Sonohata et al, 2023 ). After measuring the MEPs, atipamezole (1.5 mg/kg) was injected intraperitoneally.…”

“…The model animal research are evaluated with compound action potentials ( Hamad et al, 2022 ; Lin et al, 2018 ), behavior ( Alvites et al, 2021 ), gait analysis ( Alvites et al, 2021 ; Wang et al, 2018 ), histological evaluation ( Alvites et al, 2021 ; Wang et al, 2018 ). Further, we also investigated the impact of the nerve wrapping with both sensory thresholds and lumbar magnetic stimulation (LMS)-induced MEPs ( Sonohata et al, 2023 ).…”