Short-term adaptability to non-hyperthermal stress: Antioxidant, immune and gut microbial responses in the red swamp crayfish, Procambarus clarkii

Ruan, Guoliang; Li, Shengxuan; He, Naijuan; Liu, Fang; Wang, Qian

doi:10.1016/j.aquaculture.2022.738497

Cited by 10 publications

(4 citation statements)

References 95 publications

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“…mdpi.com/article/10.3390/antiox13050524/s1. References [63][64][65][66][67][68][69][70][71][72][73][74] are cited in the Supplementary Materials.…”

Section: Supplementary Materialsmentioning

confidence: 99%

H2O2-Induced Oxidative Stress Responses in Eriocheir sinensis: Antioxidant Defense and Immune Gene Expression Dynamics

He,

Feng,

Chen

et al. 2024

Antioxidants

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Eriocheir sinensis, a key species in China’s freshwater aquaculture, is threatened by various diseases, which were verified to be closely associated with oxidative stress. This study aimed to investigate the response of E. sinensis to hydrogen peroxide (H2O2)-induced oxidative stress to understand the biological processes behind these diseases. Crabs were exposed to different concentrations of H2O2 and their antioxidant enzyme activities and gene expressions for defense and immunity were measured. Results showed that activities of antioxidant enzymes—specificallysuperoxide dismutase (SOD), catalase (CAT), total antioxidant capacity(T-AOC), glutathione (GSH), and glutathione peroxidase (GSH-Px)—varied with exposure concentration and duration, initially increasing then decreasing. Notably, SOD, GSH-Px, and T-AOC activities dropped below control levels at 96 h. Concurrently, oxidative damage markers, including malondialdehyde (MDA), H2O2, and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels, increased with exposure duration. The mRNA expression of SOD, CAT, and GSH-Px also showed an initial increase followed by a decrease, peaking at 72 h. The upregulation of phenoloxidaseloxidase (proPO) and peroxinectin (PX) was also detected, but proPO was suppressed under high levels of H2O2. Heat shock protein 70 (HSP70) expression gradually increased with higher H2O2 concentrations, whereas induced nitrogen monoxide synthase (iNOS) was upregulated but decreased at 96 h. These findings emphasize H2O2’s significant impact on the crab’s oxidative and immune responses, highlighting the importance of understanding cellular stress responses for disease prevention and therapy development.

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“…mdpi.com/article/10.3390/antiox13050524/s1. References [63][64][65][66][67][68][69][70][71][72][73][74] are cited in the Supplementary Materials.…”

Section: Supplementary Materialsmentioning

confidence: 99%

H2O2-Induced Oxidative Stress Responses in Eriocheir sinensis: Antioxidant Defense and Immune Gene Expression Dynamics

He,

Feng,

Chen

et al. 2024

Antioxidants

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“…More specifically, increased abundance of Proteobacteria and decreased abundance of Bacteroidetes and Firmicutes was observed as the temperature elevated. However, some adaptive mechanisms were also observed as the abundance of phyla Bacteroidetes and Firmicutes, and pathogenic genera Shewanella and Acinetobacter gradually decreased while the abundance of beneficial Tenericutes and Rhodobacter gradually increased [ 125 ]. Furthermore, the effects of cadmium (Cd) at different concentrations were investigated, and from the results, it was indicated that Cd exposure could induce intestinal histological damage and affect intestinal microbiota composition and functions [ 126 ].…”

Section: Main Factors Affecting Crayfish Microbiota Abundances and Co...mentioning

confidence: 99%

Probiotics, Prebiotics, and Synbiotics Utilization in Crayfish Aquaculture and Factors Affecting Gut Microbiota

et al. 2023

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Aquaculture is affected by numerous factors that may cause various health threats that have to be controlled by the most environmentally friendly approaches. In this context, prebiotics, probiotics, and synbiotics are frequently incorporated into organisms’ feeding rations to ameliorate the health status of the host’s intestine, enhancing its functionality and physiological performance, and to confront increasing antimicrobial resistance. The first step in this direction is the understanding of the complex microbiome system of the organism in order to administer the optimal supplement, in the best concentration, and in the correct way. In the present review, pre-, pro-, and synbiotics as aquaculture additives, together with the factors affecting gut microbiome in crayfish, are discussed, combined with their future prospective outcomes. Probiotics constitute non-pathogenic bacteria, mainly focused on organisms’ energy production and efficient immune response; prebiotics constitute fiber indigestible by the host organism, which promote the preferred gastrointestinal tract microorganisms’ growth and activity towards the optimum balance between the gastrointestinal and immune system’s microbiota; whereas synbiotics constitute their combination as a blend. Among pro-, pre-, and synbiotics’ multiple benefits are boosted immunity, increased resistance towards pathogens, and overall welfare promotion. Furthermore, we reviewed the intestinal microbiota abundance and composition, which are found to be influenced by a plethora of factors, including the organism’s developmental stage, infection by pathogens, diet, environmental conditions, culture methods, and exposure to toxins. Intestinal microbial communities in crayfish exhibit high plasticity, with infections leading to reduced diversity and abundance. The addition of synbiotic supplementation seems to provide better results than probiotics and prebiotics separately; however, there are still conflicting results regarding the optimal concentration.

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“…15 Ruan conducted heat stress experiments on Procambarus clarkii and found that the AKP activity increased significantly at 12h, reached a peak, and then gradually decreased to a minimum, and the ACP activity increased significantly at 6h-12h, reached a peak, and then gradually decreased. 16 Xiong found that ACP activity in the gill tissue of P. monodon decreased significantly under high-temperature stress and combined stress of high temperature and ammonia nitrogen. 12 In this experiment, the activity of ACP in the 0mg/L group was the lowest and decreased gradually in 111mg/L, 222mg/L, and 333mg/L groups.…”

Section: Effects Of Vc On Antioxidant Capacity During Transportation ...mentioning

confidence: 99%

Effects of vitamin C on transport of Penaeus monodon

Yang

Jiang

Yang

et al. 2023

Israeli Journal of Aquaculture - Bamidgeh

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The experiment simulated the logistical transportation of Penaeus monodon. It set four distinct gradient Vitamin C (Vc) addition levels, including 0mg/L, 111mg/L, 222mg/L, and 333mg/L, to investigate the impact of Vc on the anti-stress ability of P. monodon during transportation. And the survival rate following transportation and a 15-day interim rearing period were recorded. Moreover, the alterations in tissue structure and activity of the enzymes alkaline phosphatase (ACP), alkaline phosphatase (AKP), total superoxide dismutase (T-SOD), and total superoxide dismutase (T-AOC) were checked. The results showed that with the increase of Vc supplemental level, the survival rate of P. monodon after transportation and after 15 days of temporary rearing increased to varying degrees. ACP and AKP in the hepatopancreas increased first and then decreased. T-SOD activity in the 0mg/L group was the lowest and then decreased gradually. The activity of total antioxidant capacity (T-AOC) in the 0mg/L group was the lowest and then stabilized. The branchial tissue structure also changed. The branchial tissue blood cell disorder decreased, the diaphragm gradually narrowed, the cornered cortex gradually recovered, and the swelling decreased. In conclusion, Vc positively affects the survival rate of P. monodon after transportation and temporary cultivation and alleviates the stress of P. monodon. The amount of Vc added at about 333mg/Lin, the transportation of P. monodon could play a positive role. The experimental results provide primary data for the transportation of P. monodon.

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Short-term adaptability to non-hyperthermal stress: Antioxidant, immune and gut microbial responses in the red swamp crayfish, Procambarus clarkii

Cited by 10 publications

References 95 publications

H2O2-Induced Oxidative Stress Responses in Eriocheir sinensis: Antioxidant Defense and Immune Gene Expression Dynamics

H2O2-Induced Oxidative Stress Responses in Eriocheir sinensis: Antioxidant Defense and Immune Gene Expression Dynamics

Probiotics, Prebiotics, and Synbiotics Utilization in Crayfish Aquaculture and Factors Affecting Gut Microbiota

Effects of vitamin C on transport of Penaeus monodon

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