SHORT REPORT Evaluation of a rapid test for the detection of antibodies to human immunodeficiency virus type 1 and 2

Ng, Kurtis; Saw, T.L.; Baki, Ali; Singh, Nanda Bahadur; Lyles, Cynthia M.

doi:10.1258/0956462991914320

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For usage in resource-poor laboratories, we focused on developing rapid HIV incidence tests. Commercially available rapid HIV tests were modified as less sensitive assays to identify recent HIV infection [ 16 ]. Although these modified assays can be used to estimate HIV-1 incidence, complicated steps for dilution and subtype B-derived antigen limit their practical application [ 17 – 18 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a New Limiting-Antigen Avidity Dot Immuno-Gold Filtration Assay for HIV-1 Incidence

Gao¹,

Yan²,

Xia³

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

Several laboratory assays on cross-sectional specimens for detecting recent HIV infections were developed, but these assays could not be applied in resource-limited and high HIV-incidence areas. This study describes the development of a rapid assay that can simultaneously detect the presence of HIV-1 antibodies of current and/or recent infection. The dot immuno-gold filtration assay (DIGFA) was used to detect recent infection on the principle of antibody avidity changes between recent and long-term infections. The dot immuno-gold silver staining filtration assay (DIGSSA) increases the sensitivity and accuracy of antibody detection by adding a silver staining step to the DIGFA. In the meantime the digital results were produced by the scanner for ambiguous specimens. Further, HIV-1 routine diagnostic antibody was detected simultaneously for improving practicability. The performance of the assays was then assessed through five serum panels with known serological statuses and seroconversion dates. The proportion of false recent infection (PFR) of the DIGSSA was obtained. Through the optimization of basic parameters for DIGSSA, six specimens were all classified correctly. DIGSSA demonstrated good repeatability and high sensitivity. The agreement of DIGSSA with the BED assay was 92.10% (κ = 0.65) and 95.36% with the LAg-Avidity assay (κ = 0.75). Moreover, the gray values of DIGSSA correlated well with BED ODn (R2 = 0.9397) and LAg-Avidity ODn (R2 = 0.9549). The PFR of DIGSSA was 2.73%, which was lower than that of the BED assay but higher than that of the LAg-Avidity assay. The DIGSSA can feasibly be applied to detect HIV infection and estimate HIV incidence.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of a New Limiting-Antigen Avidity Dot Immuno-Gold Filtration Assay for HIV-1 Incidence

Gao¹,

Yan²,

Xia³

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

show abstract

HIV in prison in low-income and middle-income countries

Dolan

Kite

Black

et al. 2007

The Lancet Infectious Diseases

215

172

View full text Add to dashboard Cite

Rapid Whole-Blood Finger-Stick Test for HIV Antibody: Performance and Acceptability Among Women in Northern Thailand

Liu

Kilmarx²,

Supawitkul

et al. 2003

JAIDS Journal of Acquired Immune Deficiency Syndromes

View full text Add to dashboard Cite

Although use of rapid HIV antibody tests of finger-stick blood specimens could expand voluntary counseling and testing in areas where fear of venipuncture and delays in learning test results are barriers, there is little information on performance and acceptability of these tests in Asia. We used the Hema. Strip HIV-1/2 test (Saliva Diagnostic Systems, Vancouver, WA) in a prospective cohort study of HIV seroincidence among women in northern Thailand from 1998 to 1999. Nurses obtained whole-blood specimens by finger-stick testing and provided test results and counseling at each visit. Acceptability of the rapid test was assessed at the first 6-month follow-up visit. HIV-1 seroprevalence among the 804 women screened at enrollment was 3.1%. Positive rapid test results from 25 women were confirmed by enzyme immunoassay and Western blot analysis using serum obtained by venipuncture. Of the 741 women who returned for follow-up, 56% preferred specimen collection by finger-stick testing to venipuncture, 80% preferred immediate rather than delayed test results, 79% preferred the rapid test method to typical testing methods, and 97% were satisfied with the test method used. Results from this study demonstrate the utility and acceptability of the rapid finger-stick test for HIV antibody among women in northern Thailand.

show abstract

SHORT REPORT Evaluation of a rapid test for the detection of antibodies to human immunodeficiency virus type 1 and 2

Cited by 3 publications

References 0 publications

Development of a New Limiting-Antigen Avidity Dot Immuno-Gold Filtration Assay for HIV-1 Incidence

Development of a New Limiting-Antigen Avidity Dot Immuno-Gold Filtration Assay for HIV-1 Incidence

HIV in prison in low-income and middle-income countries

Rapid Whole-Blood Finger-Stick Test for HIV Antibody: Performance and Acceptability Among Women in Northern Thailand

Contact Info

Product

Resources

About