Short Overview of Some Assays for the Measurement of Antioxidant Activity of Natural Products and Their Relevance in Dermatology

Jaganjac, Morana; Tišma, Vesna Sredoja; Žarković, Neven

doi:10.3390/molecules26175301

Cited by 17 publications

(6 citation statements)

References 119 publications

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“…Natural products with antioxidant activity have been recognized as valuable tools in the management of oxidative/nitrosative stress-induced pathologies [19]. Plants that contain certain amounts of polyphenols and flavonoids are perfect sources of antioxidants [20,21], but some studies infirmed this claim [22].…”

Section: Pharmacological Studies 221 Total Antioxidant Capacitymentioning

confidence: 99%

Antioxidant–Anti-Inflammatory Evaluation of a Polyherbal Formula

et al. 2022

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Most disease—both acute and chronic—results from inflammation, and reactive oxygen species (ROS) are considered some of the strongest stimuli of inflammation. Many studies reported the traditional use of herbal species for treating inflammation, especially when ROS are involved. The present study aims to demonstrate the antioxidant–anti-inflammatory effects of a patented preparation based on Populus nigra and Rosmarinus officinalis extracts and to highlight its applicative potential; the formula was characterized by HPTLC and HPLC and in-vitro studies were conducted on TNF-α-stimulated HUVECs. The antioxidant activity of the formula was determined by DPPH assay and the phosphomolybdenum method; to assess in-vivo anti-inflammatory activity, a rat paw edema model was used; the formula contains high amounts of polyphenols. It exhibited scavenging activity of 50–85% at 1–10 mg/mL, it inhibited nitrite production and ICAM-1 expression in TNF-α-stimulated endothelial cell cultures dose-dependently, at a maximum of 58.7% at the maximum dose administered and exerted an obvious anti-inflammatory effect in vivo, settling early and decreasing at 180 min; a new herbal bioactive product was presented with promising therapeutic potential that can be an adjunct to conventional therapies for diseases based on oxidative stress and inflammation.

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Section: Pharmacological Studies 221 Total Antioxidant Capacitymentioning

confidence: 99%

Antioxidant–Anti-Inflammatory Evaluation of a Polyherbal Formula

et al. 2022

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“…The main focus of the studies is on products prepared from plant extracts [10], essential oils [11], and microorganisms [12]. In this context, many different tests include the ferric reducing‐antioxidant power (FRAP) assay, the trolox equivalent antioxidant capacity (TEAC), the 2,3‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical technique, and the total phenolic assay are performed to determine the antioxidant potency of products extracted from these natural sources [13, 14].…”

Section: Introductionmentioning

confidence: 99%

Carotenoid characterization, fatty acid profiles, and antioxidant activities of haloarchaeal extracts

Kesbiç,

Gültepe

2023

J Basic Microbiol

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Microorganisms that can survive in saline environments, known as halotolerant or halophilic organisms, have a wide range of current and potential uses in biotechnology. In this study, it was aimed to determine the carotenoids of halophilic archaea strains isolated from the brine samples taken from different points of Salt Lake (Turkey) and determine the antioxidant activities of their carotenoids. To identify the halophilic archaea strains, they were cultivated in MAM JCM 168 medium and subjected to antibiotic susceptibility, fatty acid, two‐dimensional and three‐dimensional imaging by scanning electron microscopy and atomic force microscopy, biochemical and phylogenetic assays. The findings show that five different halophilic archaea strains have been identified as Halorubrum lipolyticum, Halorubrum sodomense, Haloarcula salaria, Halorubrum chaoviator, and Haloarcula japonica with 98% and above similarity ratio. The main fatty acids of all haloarchaeal strains were octadecanoic acid (C18:0) and palmitic acid (C16:0). The major carotenoid of the species was determined as all‐trans bacterioruberin, and different carotenoid types such as lycopene, β‐carotene, and 2‐isopentenyl‐3,4‐dehydrorodopin were found as well as bacterioruberin isomers. The antioxidant activities of carotenoids extracted from the species were analyzed by the 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging method and the extracts showed antioxidant activity statistically significantly higher than ascorbic acid and butylated hydroxytoluene as reference products (p < 0.05).

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“…It is reported that natural plant products can scavenge free radicals that result in cell death, histological damage, and chronic conditions development [ 25 ]. Hesperetin has antioxidant activity.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Antitumor Activities of Newly Synthesized Hesperetin Derivatives

et al. 2022

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Hesperetin is a class of natural products with a wide range of sources and remarkable biological activities. In this study, we described the synthesis of a series of novel hesperetin derivatives and evaluated the in vitro antioxidant and antitumor activity of these compounds. Eleven novel compounds were synthesized in moderate yields. The compounds synthesized in this work exhibited antioxidant activities against DPPH and ABTS free radicals in a dose-dependent manner. Among them, compound 3f had the best antioxidant activity, with IC50 of 1.2 μM and 24 μM for DPPH and ABTS, respectively. The antitumor activity of the compounds against human cancer cell lines, such as breast MCF-7, liver HepG2, and cervical Hela, was determined by a standard 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Three compounds had moderate IC50 values. Interestingly, compound 3f had better biological activity than hesperetin, which matches the prediction by Maestro from Schrödinger. Therefore, the new hesperidin derivative is a promising drug for the treatment of cancer due to its effective antitumor activity. The results also suggested that the antitumor activities of hesperetin derivatives may be related to their antioxidant activities.

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Short Overview of Some Assays for the Measurement of Antioxidant Activity of Natural Products and Their Relevance in Dermatology

Cited by 17 publications

References 119 publications

Antioxidant–Anti-Inflammatory Evaluation of a Polyherbal Formula

Antioxidant–Anti-Inflammatory Evaluation of a Polyherbal Formula

Carotenoid characterization, fatty acid profiles, and antioxidant activities of haloarchaeal extracts

Antioxidant and Antitumor Activities of Newly Synthesized Hesperetin Derivatives

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