Antioxidant–Anti-Inflammatory Evaluation of a Polyherbal Formula

Abstract

Most disease—both acute and chronic—results from inflammation, and reactive oxygen species (ROS) are considered some of the strongest stimuli of inflammation. Many studies reported the traditional use of herbal species for treating inflammation, especially when ROS are involved. The present study aims to demonstrate the antioxidant–anti-inflammatory effects of a patented preparation based on Populus nigra and Rosmarinus officinalis extracts and to highlight its applicative potential; the formula was characterized by HPTLC and HPLC and in-vitro studies were conducted on TNF-α-stimulated HUVECs. The antioxidant activity of the formula was determined by DPPH assay and the phosphomolybdenum method; to assess in-vivo anti-inflammatory activity, a rat paw edema model was used; the formula contains high amounts of polyphenols. It exhibited scavenging activity of 50–85% at 1–10 mg/mL, it inhibited nitrite production and ICAM-1 expression in TNF-α-stimulated endothelial cell cultures dose-dependently, at a maximum of 58.7% at the maximum dose administered and exerted an obvious anti-inflammatory effect in vivo, settling early and decreasing at 180 min; a new herbal bioactive product was presented with promising therapeutic potential that can be an adjunct to conventional therapies for diseases based on oxidative stress and inflammation.

Novel Antidiabetic Polyherbal Formulation for Synergistic Therapeutic Effects in Streptozotocin (STZ)-Induced Diabetic Rats

“…The findings of present study are similar with the findings of others that demonstrated the substantial antihyperglycemic properties of polyherbal formulation in STZ induced diabetic rats. [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] Our findings are similar with the findings of other authors. It has been demonstrated that our discoveries can decrease blood sugar levels in diabetic animals.…”

“…Compared to individual natural products, each benefit has contributed to the increased popularity of polyherbal formulation on the trade. 7,8 Azadirachta indica, Tinospora cordifolia and Oscimum sanctum are medicinal plants used for centuries to heal hyperglycemia. Thus, it was intended to explore the anti-diabetic properties of a polyherbal formulation containing different ratios of A. indica leaves, T. cordifolia stem and O. sanctum leaves.…”