Short Communication Transient increase in IL-1β, IL-6 and TNF-α gene expression in rat liver exposed to gold nanoparticles

Khan, Haseeb A.; Abdelhalim, Mohamed Anwar K; Alhomida, Abdullah S.; Ayed, M S Al

doi:10.4238/2013.november.22.12

Cited by 69 publications

(40 citation statements)

References 23 publications

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“…Elevated levels of TNF-α and decreased the anti-inflam-matory IL-4 contents in testicular and prostatic tissues are additional evidences of the aggressive cellular inflammatory response to ZnO NP exposure through the current study. Such in vivo increased TNF-α in nanoparticle exposure is in accordance with [41][42][43]. Increased TNF-α, decreased IL-4 and disturbed levels of other inflammation modulatory cytokines on exposure to nanoparticles was reported also by [42,44].…”

Section: The Mean Area % Of Masson Trichrome and Inos Immunoreactiosupporting

confidence: 77%

Toxicity of Subacute Oral Zinc Oxide Nanoparticles on Testes and Prostate of Adult Albino Rats and Role of Recovery

Mesallam¹,

Deraz²,

Aal³

et al. 2019

J Histol Histopathol

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Background: Zinc oxide nanoparticles (ZnO NPs) have immense potential and application in various types of cosmetic products and health care sectors.They have attractive properties, and attracted great interest worldwide.Yet despite its potentially interesting beneficial effects, it has been proven that they can be also cytotoxic and genotoxic for multiple types of human cells, especially the reproductive system. Aim of the work: Our study aimed to detect the effect of oral subacute ZnO NPs on the testes and prostate of adult albino rats and the role of recovery. Materials and Methods: Forty adult male albino rats were divided into three groups; control (positive and negative), ZnONP-treated group for four weeks and a similarly treated group left for supposed recovery improvement testing two weeks after cessation of ZnONP administration. All rats were examined for serum oxidative stress markers, testicular and prostatic tissue inflammatory cytokines, DNA fragmentation, and extended histopthological evaluation.Histological study was performed using hematoxylin and eosin and Masson trichrome stains. Immunohistochemical expression for iNOs was performed. Morphometric and statistical analysis were also performed. Results: Statistically significant difference in the oxidative stress and inflammatory markers and DNA shearing and aggressive histopathological testicular and prostatic changes were also detected in ZnONPtreated group as compared to the control and the follow up group that showed significant improvement.. Immunohistochemical examination of testicular and prostatic sections of ZnONP-treated group revealed highly statistically significant increase in the mean area percent of iNOs immunoreactions which significantly decreased in follow up groups. Conclusion: ZnONPs have detrimental male reproductive effects that can be controlled by limiting the exposure to such products.

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Section: The Mean Area % Of Masson Trichrome and Inos Immunoreactiosupporting

confidence: 77%

Toxicity of Subacute Oral Zinc Oxide Nanoparticles on Testes and Prostate of Adult Albino Rats and Role of Recovery

Mesallam¹,

Deraz²,

Aal³

et al. 2019

J Histol Histopathol

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“…Parts of liver samples were used to evaluate the Real-time RT-PCR for IL-1β, IL-6 and TNF-α. It was investigated as reported by Kha et al [20]. The primers sequences were IL-1β, forward (5′-CACCTTCTTTTCCTTCATCTTTG-3′) and reverse (5′-GTCGTTGCTTGTCTCTCCTTGTA -3′), IL-6, forward (5′-TGATGGATGCTTCCAAACTG-3′) and reverse (5′-GAGCATTGGAAGTTGGGGTA-3′), TNF-α, forward (5′-ACTGAACTTCGGGGTGATTG -3′) and reverse (5′-GCTTGGTGGTTTGCTACGAC-3′) and GADPH forward (5′-GTATTGGGCGCCTGGT-CACC-3′) and reverse (5′-CGCTCCTGGAAGATGGT-GATGG-3′).…”

Section: Real-time Rt-pcr For Il-1β Il-6 and Tnf-αmentioning

confidence: 99%

Carvacrol Alleivated Negative Effects of Diabetes on Inflammation and Oxidation by Modulation in Gene Expression of Inflammatory and Antioxidant System in Diabetic Rat Model

2017

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Background: Diabetes has been known as a prevalence disorder and the use of common drugs has been faced many issues with multiple limitations. This study aimed to evaluate the use of carvacrol, as a novel agent, for treatment of diabetes. Materials and Methods:A single dose of streptozotocin (55 mg/kg body weight) was used to induce the diabetes in rats. The animals were grouped into five groups including 1. Control healthy animals; 2. Diabetic controls; 3, 4 & 5. Diabetic animals given carvacrol (5, 10 and 15 mg/kg body weight/day) in neutral sterile olive oil solution oral gavage, respectively. The levels of Malondialdehyde (MDA) and catalase, Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPX) activities were evaluated. The levels of IL-1β, IL-6 and TNF-α expression in liver were assessed.Results: Administration of streptozotocin increased levels of MDA, IL-1β, IL-6 and TNF-α and also decreased activities of catalase, SOD and GPX (P<0.05). Oral administration of carvacrol, especially 15 mg/kg body weight/day, could decrease levels of MDA, IL-1β, IL-6 and TNF-α and also increase activities of catalase, SOD and GPX in comparison to diabetic control (P<0.05). Conclusion:Carvacrol could decrease or alleviate the negative effects of carvacrol on inflammation and antioxidant status that could be attributed to antioxidant properties. It could be recommended to apply carvacrol in commercial prescription in combination with other agents or as a single agent for treatment of diabetes.

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“…Gold nanoparticles (10 nm and 50 nm in size) induced IL-1 β , IL-6, and TNF- α in rat liver cells after 1 day of acute treatment and then subsided by day 5 of subchronic treatment. The 50 nm gold nanoparticles produced more severe inflammation than the 10 nm gold nanoparticles [48]. However, limited studies demonstrated whether or which TLR is involved in the NPs induced proinflammatory cytokines production.…”

Section: Nanoparticles and Immune Systemmentioning

confidence: 99%

Metal-Based Nanoparticles and the Immune System: Activation, Inflammation, and Potential Applications

Luo

Chang

Lin

2015

BioMed Research International

188

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Nanomaterials, including metal-based nanoparticles, are used for various biological and medical applications. However, metals affect immune functions in many animal species including humans. Different physical and chemical properties induce different cellular responses, such as cellular uptake and intracellular biodistribution, leading to the different immune responses. The goals of this review are to summarize and discuss the innate and adaptive immune responses triggered by metal-based nanoparticles in a variety of immune system models.

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Short Communication Transient increase in IL-1β, IL-6 and TNF-α gene expression in rat liver exposed to gold nanoparticles

Cited by 69 publications

References 23 publications

Toxicity of Subacute Oral Zinc Oxide Nanoparticles on Testes and Prostate of Adult Albino Rats and Role of Recovery

Toxicity of Subacute Oral Zinc Oxide Nanoparticles on Testes and Prostate of Adult Albino Rats and Role of Recovery

Carvacrol Alleivated Negative Effects of Diabetes on Inflammation and Oxidation by Modulation in Gene Expression of Inflammatory and Antioxidant System in Diabetic Rat Model

Metal-Based Nanoparticles and the Immune System: Activation, Inflammation, and Potential Applications

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