Short communication: Supply of methionine during late pregnancy enhances whole-blood innate immune response of Holstein calves partly through changes in mRNA abundance in polymorphonuclear leukocytes

Alharthi, Abdulrahman S.; Lopreiato, Vincenzo; Dai, Hongyu; Bucktrout, Ryan; Abdelmegeid, Mohamed; Batistel, Fernanda; Parys, C.; Shen, Xiang; Ballou, Michael; Trevisi, Erminio; Alhidary, Ibrahim A.; Abdelrahman, Mutassim M.; Loor, Juan J.

doi:10.3168/jds.2018-15676

Cited by 14 publications

(21 citation statements)

References 34 publications

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“…With regard to maternal AA supply and calf development, a nutritional strategy that our group has been investigating is the enhancement of maternal Met supply during the last 3 to 4 wk prepartum. Throughout the preweaning period, calves born to dams supplemented with RPM during late gestation had greater in vitro neutrophil phagocytosis, suggesting a priming effect of maternal Met supplementation on calf immune function ( Alharthi et al, 2019b ). Maternal Met supply also increased the hepatic activity of CBS and the concentrations of taurine compared with control calves, suggesting a greater flux of the transsulfuration pathways and a better antioxidant response with maternal Met supply ( Alharthi et al, 2019a ).…”

Section: Aa and Dairy Calvesmentioning

confidence: 99%

Amino acids and the regulation of oxidative stress and immune function in dairy cattle

Coleman

Lopreiato

Alharthi

et al. 2020

Journal of Animal Science

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Section: Aa and Dairy Calvesmentioning

confidence: 99%

Amino acids and the regulation of oxidative stress and immune function in dairy cattle

Coleman

Lopreiato

Alharthi

et al. 2020

Journal of Animal Science

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“…For instance, greater energy intake in late gestation can affect birth weight and neonatal immunocompetence [ 6 ]. Similar effects related to improvements on the immune system have been observed in offspring born to dams supplemented with adequate supplementation of trace minerals [ 7 ] and AA [ 8 , 9 ] during late pregnancy in dairy cows.…”

Section: Introductionmentioning

confidence: 54%

Gut health, stress, and immunity in neonatal dairy calves: the host side of host-pathogen interactions

Osorio

2020

J Animal Sci Biotechnol

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The cumulative evidence that perinatal events have long-lasting ripple effects through the life of livestock animals should impact future nutritional and management recommendations at the farm level. The implications of fetal programming due to malnutrition, including neonatal survival and lower birth weights, have been characterized, particularly during early and mid-gestation, when placental and early fetal stages are being developed. The accelerated fetal growth during late pregnancy has been known for some time, while the impact of maternal stressors during this time on fetal development and by extent its postnatal repercussions on health and performance are still being defined. Maternal stressors during late pregnancy cannot only influence colostrogenesis but also compromise adequate intestinal development in the fetus, thus, that further limits the newborn’s ability to absorb nutrients, bioactive compounds, and immunity (i.e., immunoglobulins, cytokines, and immune cells) from colostrum. These negative effects set the newborn calf to a challenging start in life by compromising passive immunity and intestinal maturation needed to establish a mature postnatal mucosal immune system while needing to digest and absorb nutrients in milk or milk replacer. Besides the dense-nutrient content and immunity in colostrum, it contains bioactive compounds such as growth factors, hormones, and cholesterol as well as molecular signals or instructions [e.g., microRNAs (miRNAs) and long non-coding RNAs (lncRNAs)] transferred from mother to offspring with the aim to influence postnatal gut maturation. The recent change in paradigm regarding prenatal materno-fetal microbiota inoculation and likely the presence of microbiota in the developing fetus intestine needs to be addressed in future research in ruminants. There still much to know on what prenatal or postnatal factors may predispose neonates to become susceptible to enteropathogens (e.g., enterotoxigenic Escherichia coli), causing diarrhea. From the host-side of this host-pathogen interaction, molecular data such as fecal RNA could, over time, help fill those gaps in knowledge. In addition, merging this novel fecal RNA approach with more established microbiome techniques can provide a more holistic picture of an enteropathogenesis and potentially uncover control points that can be addressed through management or nutrition at the farm level to minimize preweaning morbidity and mortality.

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“…Work from our group on maternal postruminal Met supply has generated data revealing how this essential AA can affect immune function in the neonatal calf (Jacometo et al, 2015(Jacometo et al, , 2018Alharthi et al, 2019b). The present study went further than previously to assess for the first time cobalt, FOA, and Met supply.…”

Section: Discussionmentioning

confidence: 93%

“…For instance, several studies with dietary methyl donors (e.g., methionine, choline, and folic acid) in peripartal dairy cows have confirmed benefits in performance, health, and immune function (Graulet et al, 2007;Osorio et al, 2013a;Vailati-Riboni et al, 2017b;Arshad et al, 2020). Many of these benefits were also linked to positive responses on aspects of energy metabolism, inflammation, antioxidant status, and birth BW in the offspring (Jacometo et al, 2016;Alharthi et al, 2019a;Duplessis and Girard, 2019), underscoring that adequate supply of methyl donors during later stages of fetal life is an important determinant of immunocompetence and growth in the neonatal calf (Alharthi et al, 2018(Alharthi et al, , 2019bZenobi et al, 2018).…”

Section: Introductionmentioning

confidence: 93%

“…Cellular functions of the neonatal immune system require an initial period of maturation through exposure to new antigens, with some data suggesting that nutritional status during late stages of fetal life also is critical (Hulbert and Moisá, 2016;Jacometo et al, 2018;Alharthi et al, 2019b). The role of methyl donors and 1-carbon metabolism in dairy cattle has received substantial attention over the past few decades (Girard et al, 1995;Girard and Matte, 2005;Zhou et al, 2016b).…”

Section: Introductionmentioning

confidence: 99%

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Maternal supplementation with cobalt sources, folic acid, and rumen-protected methionine and its effects on molecular and functional correlates of the immune system in neonatal Holstein calves

Lopes

Alharthi

Lopreiato

et al. 2021

Journal of Dairy Science

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Calves born to multiparous Holstein cows fed during the last 30 d of pregnancy 2 different cobalt sources [cobalt glucoheptonate (CoPro) or cobalt pectin (CoPectin)], folic acid (FOA), and rumenprotected methionine (RPM) were used to study neonatal immune responses after ex vivo lipopolysaccharide (LPS) challenge. Groups were (n = 12 calves/ group) CoPro, FOA+CoPro, FOA+CoPectin, and FOA+CoPectin+RPM. Calves were weighed at birth and blood collected at birth (before colostrum), 21 d of age, and 42 d of age (at weaning). Growth performance was recorded once a week during the first 6 wk of age. Energy metabolism, inflammation, and antioxidant status were assessed at birth through various plasma biomarkers. Whole blood was challenged with 3 µg/mL of LPS or used for phagocytosis and oxidative burst assays. Target genes evaluated by real-time quantitative PCR in whole blood samples were associated with immune response, antioxidant function, and 1-carbon metabolism. The response in mRNA abundance in LPS challenged versus nonchallenged samples was assessed via ∆ = LPS challenged − LPS nonchallenged samples. Phagocytosis capacity and oxidative burst activity were measured in neutrophils and monocytes, with data reported as ratio (percentage) of CD14 to CH138A-positive cells. Data including all time points were subjected to ANOVA using PROC MIXED in SAS 9.4 (SAS Institute Inc.), with Treatment, Sex, Age, and Treatment × Age as fixed effects. A 1-way ANOVA was used to determine differences at birth, with Treatment and Sex as fixed effects. Calf birth body weight and other growth parameters did not differ between groups. At birth, plasma haptoglobin concentration was lower in FOA+CoPro compared with CoPro calves. We detected no effect for other plasma biomarkers or immune function due to maternal treatments at birth. Compared with CoPro, in response to LPS challenge, whole blood from FOA+CoPectin and FOA+CoPectin+RPM calves had greater mRNA abundance of intercellular adhesion molecule 1 (ICAM1). No effect for other genes was detectable. Regardless of maternal treatments, sex-specific responses were observed due to greater plasma concentrations of haptoglobin, paraoxonase, total reactive oxygen metabolites, nitrite, and β-carotene in female versus male calves at birth. In contrast, whole blood from male calves had greater mRNA abundance of IRAK1, CADM1, and ITGAM in response to LPS challenge at birth. The longitudinal analysis of d 0, 21, and 42 data revealed greater bactericidal permeability-increasing protein (BPI) mRNA abundance in whole blood from FOA+CoPectin versus FOA+CoPro calves, coupled with greater abundance in FOA+CoPro compared with CoPro calves. Regardless of maternal treatments, most genes related to cytokines and cytokine receptors (IL1B, IL10, TNF, IRAK1, CXCR1), toll-like receptor pathway (TLR4, NFKB1), adhesion and migration (ICAM1, ITGAM), antimicrobial function (MPO), and antioxidant function (GPX1) were downregulated over time. Phagocytosis capacity and oxidative burst activity in both neutrophils and ...

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Short communication: Supply of methionine during late pregnancy enhances whole-blood innate immune response of Holstein calves partly through changes in mRNA abundance in polymorphonuclear leukocytes

Cited by 14 publications

References 34 publications

Amino acids and the regulation of oxidative stress and immune function in dairy cattle

Amino acids and the regulation of oxidative stress and immune function in dairy cattle

Gut health, stress, and immunity in neonatal dairy calves: the host side of host-pathogen interactions

Maternal supplementation with cobalt sources, folic acid, and rumen-protected methionine and its effects on molecular and functional correlates of the immune system in neonatal Holstein calves

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