Short communication: Search for superantigen genes in coagulase-negative staphylococci isolated from bovine milk in Canada

Corbeil, Audrey; Labrie, Josée; Goetz, Coralie; Dufour, Simon; Doghri, Ibtissem; Rivière, Loïc; Jacques, Mario

doi:10.3168/jds.2018-15648

Cited by 4 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most subclinical cases were associated with S. chromogenes (15.6%) followed by S. simulans (6.8%), S. xylosus (6.5%), S. haemolyticus (6.3%), S. cohnii (5.8%), S. epidermidis (5.5%), S. capitis (5.3%), S. sciuri (5.3%), S. gallinarum (5.0%), S. warneri (4.8%), S. equorum (4.5%), S. saprophyticus (4.0%), S. succinus (3.8%), S. arlettae (3.5%), and S. agnetis (3.3%). These findings are consistent with a growing number of studies which report that coagulase-negative staphylococci are emerging pathogens associated with mastitis and persistence of intramammary infection in bovine worldwide 7 . As observed in this study, in a recent Canadian study, the S. chromogenes and S. simulans were among the most common species found in clinical mastitis cases 8 .…”

Section: Discussionsupporting

confidence: 92%

Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes

Pizauro

Almeida

Silva

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Mastitis is a common and costly disease on dairy farms, commonly caused by Staphylococcus spp. though the various species are associated with different clinical outcomes. In the current study, we performed genomic analyses to determine the prevalence of adhesion, biofilm, and related regulatory genes in 478 staphylococcal species isolated from clinical and subclinical mastitis cases deposited in public databases. The most prevalent adhesin genes (ebpS, atl, pls, sasH and sasF) were found in both clinical and subclinical isolates. However, the ebpS gene was absent in subclinical isolates of Staphylococcus arlettae, S. succinus, S. sciuri, S. equorun, S. galinarum, and S. saprophyticus. In contrast, the coa, eap, emp, efb, and vWbp genes were present more frequently in clinical (vs. subclincal) mastitis isolates and were highly correlated with the presence of the biofim operon (icaABCD) and its transcriptional regulator, icaR. Co-phylogenetic analyses suggested that many of these adhesins, biofilm, and associated regulatory genes could have been horizontally disseminated between clinical and subclinical isolates. Our results further suggest that several adhesins, biofilm, and related regulatory genes, which have been overlooked in previous studies, may be of use for virulence profiling of mastitis-related Staphylococcus strains or as potential targets for vaccine development.

show abstract

Section: Discussionsupporting

confidence: 92%

Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes

Pizauro

Almeida

Silva

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In our study, only 5% of the 163 CNS bovine milk isolates were enterotoxin gene positive, with all 8 of the positive CNS strains harboring the seg gene only. The results of other studies, assaying different numbers and types of enterotoxin genes and applying different analytical methods, indicated that 0 to 80% of CNS strains tested were potentially enterotoxigenic (Nemati et al, 2008;Park et al, 2011;Fijałkowski et al, 2014;Bertelloni et al, 2015;Siqueira et al, 2017;Corbeil et al, 2019).…”

Section: Short Communicationmentioning

confidence: 99%

“…Screening 71 CNS bovine isolates for 12 enterotoxin genes (sea, seb, sec, sed, seg, selk, sell, selm, seln, selo, selp, selq) by single PCR (Corbeil et al, 2019) revealed the presence of 27 amplicons matching those typical for selo (9), sec (8), seg (5), seb (4), and sell (1) genes. However, when assessed by sequencing, none of these amplicons was confirmed as an enterotoxin gene.…”

Section: Short Communicationmentioning

confidence: 99%

Short communication: Enterotoxigenic potential of coagulase-negative staphylococci isolated from bovine milk in Poland

Helak

Daczkowska-Kozon

Dłubała

2020

Journal of Dairy Science

View full text Add to dashboard Cite

“…Despite being considered minor mastitis pathogens [ 17 ], the importance of CoNS as a causative agent of intramammary infections in cattle is growing [ 18 ]. These may have coding genes for the biofilm formation, hemolysins, exoenzymes and superantigens [ 19 ], however, there is little information about their virulence factors [ 20 ] and the different immune response of the host to intramammary infections caused by environmental species or adapted to the host [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis

et al. 2022

View full text Add to dashboard Cite

Background Mastitis is one of the major diseases in dairy cattle, as it causes great economic losses to producers due to the reduction of milk production and changes in the quality of the product. The disease is mainly caused by bacteria of the genus Staphylococcus spp., these microorganisms can express various virulence factors, such as biofilms for example. In herds with organic management, producers and technicians use unconventional ways to treat and control the disease, such as homeopathy. However, it is not known if this type of treatment is able to control pathogenic bacteria such as those of the genus Staphylococcus, of relevance to animal and human health. Thus, the objective of this study was to investigate the production of biofilm in vitro and its genes by Staphylococcus spp. isolated in the milk of cows treated with homeopathy, as well as the persistence of microorganisms in animals. Methods Ninety-nine isolates of Staphylococcus spp. from cows treated and not treated with homeopathy were identified by internal transcribed space-polymerase chain reaction and investigated for the presence of the icaABCD, bap, aap, atlE, and bhp genes and in vitro biofilm production using the adhesion method on polystyrene plates. The enzyme restriction profile was determined by Pulsed-Field Gel Electrophoresis. Clusters of S. aureus and S. epidermidis with three or more isolates had an isolate selected for Multilocus Sequence Typing. Results The frequency of S. aureus isolations was similar in treated and untreated cows, while 71.4% of the coagulase-negative identified were isolated in cows treated with homeopathy. The distribution of the operon ica genes was similar in animals with and without treatment, except for the icaD gene, more frequent in treated cows. Production of biofilm was associated with presence of one or more genes from the icaADBC operon. S. aureus revealed a greater diversity and greater dissemination in cows treated and not treated with homeopathy. Sequence Types ST1, ST5, and ST126 were identified in S. aureus. Conclusions The presence of biofilm-associated genes and the in vitro production of biofilms, combined with the persistence of clonal profiles of Staphylococcus spp. demonstrate other forms of control for bovine mastitis should be researched for organic production herds.

show abstract

Short communication: Search for superantigen genes in coagulase-negative staphylococci isolated from bovine milk in Canada

Cited by 4 publications

References 17 publications

Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes

Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes

Short communication: Enterotoxigenic potential of coagulase-negative staphylococci isolated from bovine milk in Poland

Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis

Contact Info

Product

Resources

About