Short communication: Enterotoxigenic potential of coagulase-negative staphylococci isolated from bovine milk in Poland

Helak, I.; Daczkowska-Kozon, E.; Dłubała, Alicja

doi:10.3168/jds.2019-17574

Cited by 9 publications

(7 citation statements)

References 23 publications

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“…genes detection rate than the other enterotoxin genes in CoNS species-induced bovine mastitis. However, the result was different from the reports of Chajecka-Wierzchowska et al [42], Helak et al [43] who reported no classical enterotoxin genes detected from CoNS isolates.…”

Section: Discussioncontrasting

confidence: 94%

Coagulase-negative Staphylococci from Bovine Milk: Virulent gene detection, and Antibiogram Profiles

Getahun,

Abey,

Beyene

et al. 2023

Preprint

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Background Coagulase-negative Staphylococcus species are an emerging cause of intramammary infection, posing a significant economic and public health threat. The cross-sectional study was conducted to isolate and assess virulence factors and antibiogram profiles of coagulase-negative Staphylococcus species in 290 milk samples collected from February to August 2022. Species isolation and identification were performed by plate culturing and biochemical tests and single-plex PCR was used for the detection of virulent genes. The antimicrobial susceptibility profile of each coagulase-negative Staphylococcus species isolate was determined by the Kirby-Bauer disc diffusion test. Results Coagulase-negative Staphylococcus species were isolated in 28.6%, (83/290) of the samples. Of these, the S. epidermidis, S. sciuri, S. warneri, S. haemolyticus, S. simulans, S. chromogens, S. cohnii, and S. captis species were isolated at the rates of 11, 5.2, 3.4, 3.1, 3.1, 1, 1, and 0.7% respectively. Coagulase-negative Staphylococcus species carried 26.5, 22.1, 21.7, 9.6, 9.6 and 8.4% of the ica D, Pvl, Mec A, hlb, Sec, and hla virulent genes respectively. All the isolates showed a high percentage (100%) of resistance to Amoxicillin, Ampicillin, and Cefotetan and 37.5% of resistance to Oxacillin. The majority (54.2%) of coagulase-negative isolates also showed multidrug resistance. Conclusion The present study revealed that coagulase-negative Staphylococcus species isolates were found carriage of one or more virulent and enterotoxin genes responsible for intramammary and food poisoning infections. The majority of the isolates (54.2%) were also found multidrug resistant. Thus, urgent disease control and prevention measures are warranted to reduce the deleterious impact of coagulase-negative species. This study is the first to document the detection of virulent and food poisoning genes in coagulase-negative Staphylococcus species from milk in Ethiopia.

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Section: Discussioncontrasting

confidence: 94%

Coagulase-negative Staphylococci from Bovine Milk: Virulent gene detection, and Antibiogram Profiles

Getahun,

Abey,

Beyene

et al. 2023

Preprint

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“…S. aureus isolates harbouring various toxin and virulence genes have been recovered from retail aquatic products including shrimp (Rong et al ., 2017; Beshiru et al ., 2021). The enterotoxigenic potential of coagulase‐negative staphylococci has been reported previously in isolates recovered from various sources including ready‐to‐eat food and bovine milk (Chajęcka‐Wierzchowska et al ., 2020; Helek et al ., 2020). However, in general, the ability of CoNS to secrete classical staphylococcal enterotoxins (SEs) or toxic shock syndrome toxins (TSST) in significant amount to cause food poisoning or TSS has not been proven (Becker et al ., 2014).…”

Section: Resultsmentioning

confidence: 99%

Genotypes and phenotypes of methicillin‐resistant staphylococci isolated from shrimp aquaculture farms

Rajan

Sivaraman

Vijayan

et al. 2021

Environ Microbiol Rep

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Summary The population of methicillin‐resistant (MR) staphylococci in aquatic environment is rarely investigated. Here, we characterized a collection of MR staphylococci recovered from shrimp aquaculture farms (n = 37) in Kerala, India. A total of 261 samples yielded 47 MR isolates (16 S. aureus, 13 S. haemolyticus, 11 S. epidermidis, 3 S. saprophytics and 2 each of S.intermedius and S. kloosii). Multi‐drug resistance was evident in 72.3% of the isolates, with resistance mainly towards erythromycin (78.7%), norfloxacin and trimethoprim‐sulfamethoxazole (53.2%), and gentamicin (34%). Major resistance genes identified included mecA (100%), ermC (38.3%), aacA‐aphD (21.3%), tetK (14.9%) and tetM (21.3%). Almost 60% of the isolates carried type V SCCmec (Staphylococcal Cassette Chromosome mec), and the remaining harboured untypeable SCCmec elements. Comprehensive genotyping of the methicillin‐resistant Staphylococcus aureus isolates revealed high prevalence of ST772‐t345‐V (sequence type‐spa type‐SCCmec type) (75%), followed by minor representations of ST6657‐t345‐V and ST3190‐t12353. The isolates of S. haemolyticus and S. epidermidis were genotypically diverse as shown by their pulsed‐field gel electrophoresis (PFGE) profiles. Genes encoding staphylococcal enterotoxins were observed in 53.2% of the isolates. Various genes involved in adhesion and biofilm formation were also identified. In conclusion, our findings provide evidence that shrimp aquaculture settings can act as reservoirs of methicillin‐resistant staphylococci.

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“…The literature data points to the increasing prevalence of various virulence factors in bacteria, particularly CoNS. This includes the identification of genes responsible for the production of staphylococcal enterotoxins in CoNS [ 2 , 15 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. In the study, the presence of one or more genes that encode the ability to produce enterotoxins in a total of 73 (97.3%) isolates was found, including all S .…”

Section: Discussionmentioning

confidence: 99%

Identification of the Enterotoxigenic Potential of Staphylococcus spp. from Raw Milk and Raw Milk Cheeses

Wiśniewski,

Gajewska,

Zadernowska

et al. 2023

Toxins

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This study aimed to genotypic and phenotypic analyses of the enterotoxigenic potential of Staphylococcus spp. isolated from raw milk and raw milk cheeses. The presence of genes encoding staphylococcal enterotoxins (SEs), including the classical enterotoxins (sea-see), non-classical enterotoxins (seg-seu), exfoliative toxins (eta-etd) and toxic shock syndrome toxin-1 (tst-1) were investigated. Isolates positive for classical enterotoxin genes were then tested by SET-RPLA methods for toxin expression. Out of 75 Staphylococcus spp. (19 Staphylococcus aureus and 56 CoNS) isolates from raw milk (49/65.3%) and raw milk cheese samples (26/34.7%), the presence of enterotoxin genes was confirmed in 73 (97.3%) of them. Only one isolate from cheese sample (1.3%) was able to produce enterotoxin (SED). The presence of up to eight different genes encoding enterotoxins was determined simultaneously in the staphylococcal genome. The most common toxin gene combination was sek, eta present in fourteen isolates (18.7%). The tst-1 gene was present in each of the analyzed isolates from cheese samples (26/34.7%). Non-classical enterotoxins were much more frequently identified in the genome of staphylococcal isolates than classical SEs. The current research also showed that genes tagged in S. aureus were also identified in CoNS, and the total number of different genes detected in CoNS was seven times higher than in S. aureus. The obtained results indicate that, in many cases, the presence of a gene in Staphylococcus spp. is not synonymous with the ability of enterotoxins production. The differences in the number of isolates with genes encoding SEs and enterotoxin production may be mainly due to the limit of detection of the toxin production method used. This indicates the need to use high specificity and sensitivity methods for detecting enterotoxin in future studies.

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Short communication: Enterotoxigenic potential of coagulase-negative staphylococci isolated from bovine milk in Poland

Cited by 9 publications

References 23 publications

Coagulase-negative Staphylococci from Bovine Milk: Virulent gene detection, and Antibiogram Profiles

Coagulase-negative Staphylococci from Bovine Milk: Virulent gene detection, and Antibiogram Profiles

Genotypes and phenotypes of methicillin‐resistant staphylococci isolated from shrimp aquaculture farms

Identification of the Enterotoxigenic Potential of Staphylococcus spp. from Raw Milk and Raw Milk Cheeses

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