Short Communication: Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography

Arifiyanto, Deni; Basyuni, Mohammad; Sumardi, Sumardi; Putri, Lollie Agustina P.; Siregar, Etti Sartina; Risnasari, Iwan; Syahputra, Indra

doi:10.13057/biodiv/d180426

Cited by 25 publications

(13 citation statements)

References 16 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results of isoprenoid analysis in oil palm leaf tissue (Table 1) showed that leaf tissue of oil palm has only type II lipid pattern. These findings supported the previous study by Arifiyanto et al (2017) that type II lipid was present in oil palm Dura, Pisifera, and Tenera, oil palm infected with Ganoderma boninense (Afandy et al 2019), oil palm in different land use (Basyuni et al 2018).…”

Section: Polyisoprenoids Analysis By Two-dimensional Thin-layer Chromatographysupporting

confidence: 91%

“…The results showed that leaf tissue and mesocarp had various carbon chain lengths. The length of the polyisoprenoid carbon chains in oil palms varies according to tissue type although in the same species and forms a particular dominant polyisoprenoid family (Arifiyanto et al 2017). The difference in the length of the polyisoprenoid carbon chain in oil palm leaves (E. guineensis) is caused by several factors, including the age of tissue (tissue aging), salinity stress, tissue differences (Basyuni et al 2018).…”

Section: Quantification Of Polyisoprenoid Compoundsmentioning

confidence: 99%

See 1 more Smart Citation

Distribution of polyprenol and dolichol in oil palm genotype (Elaeis guineensis) involving lipase activity

et al. 2021

Self Cite

View full text Add to dashboard Cite

Abstract. Habsyah TS, Basyuni M, Siregar LAM, Afandi D, Syahputra I. 2021. Distribution of polyprenol and dolichol in oil palm genotype (Elaeis guineensis) involving lipase activity. Biodiversitas 22: 830-837. The aim of this study was to determine the pattern of chemical compounds from the polyisoprenoid group in oil palm plants that were contributing to the lipase activity. The study was conducted in two locations. A sampling of leaves and fruit mesocarp from 90 progenies was conducted at PT. Socfin Indonesia Aek Loba Plantation, Asahan District, North Sumatra, Indonesia. Biochemical analysis of polyisoprenoid was carried out at the Forest Biotechnology Laboratory, Faculty of Forestry, University of Sumatera Utara, Medan. The results showed that the total lipids in leaves tissue ranged from 0.40 to 1.032 mg/g, with the lowest total lipid found in SL-4966 progeny and the highest total lipid found in SL-9737 progeny. Polyisoprenoid values ranged from 1.63 to 10.82 mg/g dry weight. The highest polyisoprenoid content was obtained in SL-10682 progeny, and the lowest was in SL-5045 progeny leaf tissue. The polyisoprenoid in fruit mesocarp tissue ranged from 1.79 to 4.14 mg/g. The lowest polyisoprenoid content (1.79 mg/g) of fruit mesocarp was found in SL-4233 progeny, while the highest (4.14 mg/g) was found in SL-5010 progeny. Dolichol and polyprenol are equally dominant in palm leaves. Carbon polyprenol chain lengths ranged from C45-C105, and dolichol ranged from C50-C110. The polyprenol carbon chain lengths in the mesocarp tissues were C65-C120 and dolichol was C65-C125. Polyisoprenoid analysis on oil palm leaf and fruit mesocarp tissue showed that lipid patterns of oil palm leaf and mesocarp were categorized as type II lipid patterns. It provides important information for selecting genotypes of low lipase activity of oil palm plants.

show abstract

Section: Polyisoprenoids Analysis By Two-dimensional Thin-layer Chromatographysupporting

confidence: 91%

Section: Quantification Of Polyisoprenoid Compoundsmentioning

confidence: 99%

Distribution of polyprenol and dolichol in oil palm genotype (Elaeis guineensis) involving lipase activity

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Beberapa bakteri alami Indonesia berhasil diisolasi dari daerah tercemar dan memiliki kemampuan akumulasi tembaga. Bakteri ini dapat bertindak sebagai biosorben sehingga dapat menyisihkan tembaga dari lingkungan (5) . Populasi bakteri di daerah yang tercemar logam berat akan mengembangkan suatu proses adaptasi untuk menjadi resisten terhadap logam berat.…”

Section: Latar Belakangunclassified

“…Koloni bakteri diinokulasikan ke dalam medium LB padat yang diberi CuSO4 konsentrasi terntentu kemudian diinkubasikan pada suhu 37 o C selama dua hari. Koloni yang tumbuh diuji lagi pada konsentrasi CuSO4 yang lebih tinggi hingga isolat bakteri tidak mampu tumbuh (5) . Bakteri yang paling resisten dipilih dan digunakan untuk isolasi plasmid dan analisis gen 16s rDNA.…”

Section: Uji Resistensiunclassified

Amplifikasi Gen Resistensi Tembaga (Cur) pada Bakteri yang Diisolasi dari Limbah Industri di Surabaya

Irawati

2020

Jurtekling

View full text Add to dashboard Cite

Copper pollution is one of the serious environmental problems in Indonesia. Higher concentration of copper is toxic so that threat living organism. Bio-remediation using copper resistant bacteria is effective for solving heavy metals pollution because the bacteria adapt easily when applied in environment. Using bacteria containing gene encoded copper resistance could help effort of copper bio-remediation. The purpose of this research is to isolate and characterize copper resistant bacteria from industrial sewage in Surabaya also to amplification of gene encoded resistance to copper (Cur). Bacteria was grown on Salts Base Solution medium with addition of appropriate concentration of copper. Resistance to copper was determined based on Minimum Inhibitory Concentration of CuSO4. Molecular characterization was done based on 16S rDNA gene analysis using universal primer. Amplification of Cur gene was done using specific primer of gene orf3, orf4, orf5 from Lactoccocus lactis. Three highly copper resistant bacteria have been isolated with the MIC of 2.5-7.5 mM CuSO4 encoded IrC1, IrC2, and IrC4. Isolate IrC4 is the highest copper resistant bacteria with the MIC of 7.5 mM. Resistance mechanism may be through accumulation copper inside the cells with the total of 371 mg/g dry weight of cells. The three bacteria have plasmid with the size of 21 kb. Isolate IrC4 have 96.99% similarity with Cupriavidus pauculus. Amplification of copper-resistance (Cur) gene demonstrated that a single band of 0.9 kb was obtained from isolate C4. The finding of indigenous resistant bacteria encoded Cur gene may give better solution for pollution problem in Indonesia.Keywords: bacteria, copper, Isolate IrC4, Lactococcus lactis, resistantABSTRAKPencemaran tembaga di Indonesia merupakan salah satu masalah lingkungan yang serius sehingga perlu diatasi. Tembaga pada konsentrasi yang tinggi bersifat toksik sehingga mengancam kehidupan organisme. Bioremediasi menggunakan bakteri resisten tembaga yang diisolasi dari lingkungan tercemar terbukti efektif mengatasi pencemaran logam berat karena lebih mudah beradaptasi ketika diterapkan di lingkungan. Pemanfaatan bakteri yang mengandung gen penyandi resistensi tembaga dapat menunjang keberhasilan usaha bioremediasi tembaga. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi bakteri resisten tembaga dari limbah industri di Surabaya serta melakukan amplifikasi gen yang menyandi resistensi bakteri tersebut terhadap tembaga (Cur). Isolasi bakteri dilakukan menggunakan medium Salt Base Solution dengan penambahan CuSO4. Uji resistensi ditentukan berdasarkan nilai Minimum Inhibitory Concentration (MIC) terhadap CuSO4. Karakterisasi molekular dilakukan berdasarkan analisis gen 16S rDNA menggunakan primer universal. Amplifikasi gen Cur dilakukan dengan menggunakan primer yang spesifik terhadap gen orf3, orf4, orf5 dari gen Cur pada Lactobacillus lactis. Hasil penelitian menunjukkan bahwa terdapat tiga isolat bakteri yang paling resisten dengan nilai MIC= 6.5-7.5 mM, yaitu isolat IrC1, IrC2, dan IrC4. isolat IrC4 merupakan isolat bakteri yang paling resisten dengan nilai MIC sebesar 7.5 mM. Mekanisme reistensi isolat IrC4 adalah dengan cara mengakumulasi tembaga di dalam sel selitar 371 mg/g berat kering sel. Ketiga isolat memiliki plasmid berukuran sekitar 21 kb. Analisis gen 16S rDNA menunjukkan bahwa isolat IrC4 memiliki kemiripan 96,99% dengan Cupriavidus pauculus. Gen Cur pada isolat IrC4 sepanjang 0.9 kb berhasil diamplifikasi dengan menggunakan primer spesifik gen orf3 yang menyandikan pengikatan tembaga. Penemuan bakteri indigen indonesia yang menyandikan gen resisten tembaga diharapkan dapat menunjang keberhasilan penanganan masalah pencemaran tembaga di Indonesia.Kata kunci: bakteri, isolat IrC4, Lactobacillus lactis, resisten, tembaga.

show abstract

“…Dolichol is present in nearly all plant tissues, particularly in the roots (Basyuni et al 2016(Basyuni et al , 2017a(Basyuni et al , b, 2018a, from the shorter (C25-C40), medium-chain (C65-C85), and longer chain length (C90-C140). In Arifiyanto et al (2017) research, the length of the polyisoprenoid carbon chain in oil palms varies according to each tissue or organ even though it is in the same species but comes from different growth phases and the type of fruit produced.…”

Section: Introductionmentioning

confidence: 99%

Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation

2021

Self Cite

View full text Add to dashboard Cite

Abstract. Kartika EBA, Siregar LAM, Basyuni M. 2021. Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation. Biodiversitas 22: 3423-3436. Oil palm tissue culture is carried out through indirect embryogenesis, which causes somaclonal diversity to occur at the in vitro propagation stage, especially in the callus growth phase. In the cells of all living organisms can be found a group of polyisoprenoid compounds. This study aims to determine variations in oil palm plants resulting from tissue culture based on the presence of polyisoprenoid compounds. Oil palm leaf samples (Elaeis guineensis Jacq.) were collected from the plantation of PT. Socfin Indonesia, North Sumatra, Indonesia. Sample extraction, saponification, isolation of polyisoprenoid compounds, and two-dimensional thin-layer chromatography analysis were carried out to obtain data related to total lipids, polyprenol and dolichol profile, and Carbon (C) chain length polyprenol and dolichol from oil palm leaves of Pisifera parents and their propagated derivatives by indirect embryogenesis. The results showed that the amount of polyisoprenoid mother S24 was 2.41 mg/g higher than that of the parentS8 at 2.17 mg/g dry weight. Total polyisoprenoid fromS8 plants in vitro ranged from 0.71 - 8.53 mg/g dry weight, with the lowest total polyisoprenoid found atS894/22 and the highest was found atS893/2. While for total polyisoprenoid from plant tissue culture of Pisifera S24 ranged from 0.73 - 8.05 mg/g dry weight, with the lowest total polyisoprenoid found at S24H14 and the highest was found at S24H16. The parent plants of Pisifera S8 and S24, as well as plants resulting from tissue culture, were categorized as having lipid pattern type II, which showed a balanced distribution of polyprenol with dolichol. The longest carbon chain was found in vitro plantsS8 93/4 ranged from C50-C110, while the shortest was found in plants produced in vitro S24H7 starting from C45-C55. There were variations in the carbon chain length of polyprenol and dolichol in the leaf samples derived from in vitro propagation of the Pisifera S8 and S24 parents.

show abstract

Short Communication: Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography

Cited by 25 publications

References 16 publications

Distribution of polyprenol and dolichol in oil palm genotype (Elaeis guineensis) involving lipase activity

Distribution of polyprenol and dolichol in oil palm genotype (Elaeis guineensis) involving lipase activity

Amplifikasi Gen Resistensi Tembaga (Cur) pada Bakteri yang Diisolasi dari Limbah Industri di Surabaya

Distribution of polyprenol and dolichol in oil palms from Pisifera parents and mature plants from tissue culture propagation

Contact Info

Product

Resources

About