Short communication. Nitrate reductase activity in roots and leaves of chickpea cultivars under salt stress

Garg, Neera; Singla, Ranju

doi:10.5424/sjar/2005032-149

Cited by 10 publications

(7 citation statements)

References 8 publications

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“…Many factors influence how salt affects NR activity including plant species, nitrogen supply availability, salt content, and time duration of stress exposure to plants. Like present observation, exposure to increasing levels of NaCl significantly decreased the activity of NR in roots and leaf tissues of various agriculturally important vegetable crops including Solanum lycopersicum L. (tomato), Cucumis sativus L. (cucumber), 49 Zea mays L. (maize), 50 Beta vulgaris L. (sugar beet), 51 V. radiata L. (green gram), 52 Cicer arietinum L. (chickpea), 53 etc.…”

Section: Resultssupporting

confidence: 79%

Sodium Chloride (NaCl)-Induced Physiological Alteration and Oxidative Stress Generation in Pisum sativum (L.): A Toxicity Assessment

Alharbi

Alosaimi

Alghamdi³

2022

ACS Omega

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Salinity stress has a deleterious impact on plant development, morphology, physiology, and biochemical characteristics. Considering the NaCl-induced phytotoxicity, current investigation was done to better understand the salt-tolerant mechanisms using Pisum sativum L. (pea) as a model crop. Generally, NaCl resulted in a progressive decrease in germinative attributes and physiological and biochemical parameters of P. sativum (L.). The 400 mM NaCl level had a higher detrimental effect and reduced the germination rate, plumule, radicle length, and seedling vigor index (SVI) by 78, 89, 84, and 77%, respectively, under in vitro . Furthermore, after 400 mM NaCl exposure, physiological and enzymatic profiles like root dry biomass (71%) chl-a (66%), chl-b (54%), total chlorophyll (45%), and nitrate reductase activity (NRA) (59%) of peas were decreased. In addition, a NaCl dose-related increase in soluble protein (SP) and sugar (SS), Na + and K + ions, and stressor metabolites was recorded. For instance, at 400 mM NaCl, SP, SS, Na + ion, K + ion, root proline, and malondialdehyde (MDA) contents were significantly and maximally elevated by 65, 33, 84, 79, 85, and 89%, respectively, compared to the control (0 mM NaCl). Data analysis indicated that greater doses of pesticides dramatically increased reactive oxygen species (ROS) levels and induced membrane damage through production of thiobarbituric acid reactive substances (TBARS), as well as increased cell injury. To deal with NaCl-induced oxidative stress, plants subjected to higher salinity stress showed a considerable build-up in antioxidant levels. As an example, ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were maximally and significantly ( p ≤ 0.05) increased by 68, 80, 74, and 58%, respectively, after 400 mM NaCl exposure. The propidium iodide (PI)-stained and NaCl-treated plant roots corroborated the damaging effect of salinity-induced stress on the cell membrane, which was observed under a confocal laser microscope (CLSM). The cells exposed to 400 mM NaCl had maximum fluorescence intensity, indicating that higher level of salts can cause pronounced cell damage and reactive oxygen species (ROS) generation. The increases in superoxide ion (O 2 – ) and hydrogen peroxide (H 2 O 2 ) content in NaCl-treated plant tissues indicated the elevation of ROS with increasing salt levels. This finding revealed that salt stress can cause toxicity in plants by causing alteration in metabolic activity, oxidative injury, and damage to cell membrane integrity.

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Section: Resultssupporting

confidence: 79%

Sodium Chloride (NaCl)-Induced Physiological Alteration and Oxidative Stress Generation in Pisum sativum (L.): A Toxicity Assessment

Alharbi

Alosaimi

Alghamdi³

2022

ACS Omega

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“…Nitrate reductase enzyme is substrate inducible enzyme, its' induction is closely dependent on the availability of nitrate and the enzyme is found in cytosol of plant cells in leaves. The results are in close agreement with Garg and Singla (2005) who reported that nitrate reductase activity in the leaves in the chickpea cultivars decreased however maximum activity of this enzyme was observed after 70 days after sowing. Similar trend were also observed in the present investigation.…”

Section: Resultssupporting

confidence: 82%

“…NR and NiR enzymes are sensitive to alkalinity and their activities decreased with increasing level of alkalinity in the soil. The results are in close agreement with Garg and Singla (2005). Nitrogenase activity generally increased with plant age or decreased at flowering stage (Bidlack et al, 2001).…”

Section: Resultssupporting

confidence: 80%

The Possible Involvement of Exogenous Gibberellic Acid on Nitrogen Assimilating Enzyme Activity and Total Nitrogen Content in Chickpea (Cicer aeritinum L.) Genotypes

Arya¹,

Arya²,

Singh³

2018

Int.J.Curr.Microbiol.App.Sci

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“…Plant nitrogen conditions were no different in the various clones tested. In research Garg and Singla (2005), there was a decrease in NRA under saline conditions causing the degradation of nitrate reductase or decreasing in enzymes synthesis rate. NRA reduction in leaf and root occurred proportionally to the reduction of the dry weight root and shoot accumulation.…”

Section: Discussionmentioning

confidence: 99%

Root detection by resistivity imaging and physiological activity with the dead-end trench on three clones of cocoa (Theobroma cacao)

Nailil

Putra

2020

Biodiversitas

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Abstract. Maghfiroh CN, Putra ETS, Dewi HSES. 2020. Root detection by resistivity imaging and physiological activity with the dead-end trench on three clones of cocoa (Theobroma cacao). Biodiversitas 21: 2794-2803. Indonesia is one of the largest cocoa production countries in Southeast Asia, but has low average productivity (982 kg/ha) based on Indonesian Plantation Statistics (2017). Productivity increase effort by giving dead-end trenches that applied to collect organic material, accommodate surface runoff sediments, prevent erosion, and maintain nutrient availability. Dead-end trench had an impact on root cutting and improve rooting. Clone is very influential on cocoa (Theobroma cacao L.) yield. The superior clones recommended are clones RCC-70, RCC-71, and KKM-22, which have high productivity and are resistant to pests and diseases. The objectives of this research were (i) to detect the presence of roots by resistivity imaging (ii) to study the effects of dead-end trench application on physiological activities of cocoa leaves and yields of three clones (RCC-70, RCC-71, and KKM-22); (iii) to determine which cocoa clone (s) performed a significant yield increase with the application of dead-end trench. Research was conducted in August 2018-April 2019 at PT. Pagilaran cocoa plantation in North Segayung Production Unit, subdistrict Tulis, Batang, Central Java. The experiment was arranged in a randomized complete design with two factors and three blocks as replications. The first factor was dead-end trench application (with or without dead-end trench application) and the second factor was cocoa clones (RCC-70, RCC-71, and KKM-22). This study showed that dead-end trench applications affected plant roots based on the interpretation results of geoelectric-resistivity measurements. Dead-end trench application significantly affected chlorophyll a, stomatal conductance, transpiration, and H2O leaf content. Dead-end trench application has no significant effect on the content of chlorophyll b, total chlorophyll content, nitrate reductase activity, stomatal density, CO2 leaf content, and photosynthesis rate.

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Short communication. Nitrate reductase activity in roots and leaves of chickpea cultivars under salt stress

Cited by 10 publications

References 8 publications

Sodium Chloride (NaCl)-Induced Physiological Alteration and Oxidative Stress Generation in Pisum sativum (L.): A Toxicity Assessment

Sodium Chloride (NaCl)-Induced Physiological Alteration and Oxidative Stress Generation in Pisum sativum (L.): A Toxicity Assessment

The Possible Involvement of Exogenous Gibberellic Acid on Nitrogen Assimilating Enzyme Activity and Total Nitrogen Content in Chickpea (Cicer aeritinum L.) Genotypes

Root detection by resistivity imaging and physiological activity with the dead-end trench on three clones of cocoa (Theobroma cacao)

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