Short Communication:  New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus

Virgianti, Dewi Peti; Natalia, Desi; Aryantha, I Nyoman Pugeg

doi:10.13057/biodiv/d210449

Cited by 4 publications

(3 citation statements)

References 37 publications

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“…In the chrome azurol S (CAS) agar test, the isolate P. aeruginosa N1 had the largest zone (data not shown). Identification of P. aeruginosa N1 was performed based on a molecular technique using 27F and 1492R primers (Virgianti et al, 2020). The genomic DNA was extracted using the Quick-DNATM Fungal/Bacterial Miniprep Kit (Zymo Research; D6005), and polymerase chain reaction (PCR) was performed using a Thermocycler Agilent SureCycler 8800.…”

Section: Isolation and Identification Of Associated Bacteria Causing ...mentioning

confidence: 99%

Phenazine and quinolone are main compounds produced by Pseudomonas aeruginosa N1 during inhibitory interaction with Rhizopus microsporus

2023

ANRES

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Importance of the work: Pseudomonas aeruginosa N1 inhibits Rhizopus microsporus growth by the production of quinolone, phenazine and rhamnolipid. Objectives: To evaluate the compounds extracted from P. aeruginosa N1 monoculture and from co-culture between R. microsporus and P. aeruginosa N1 and to observe the degree of damage caused to R. microsporus by its inhibitory interaction with P. aeruginosa N1. Materials & Methods:The inhibitory interaction of P. aeruginosa N1 was studied based on the metabolite released during R. microsporus inhibition. The substance released during the inhibitory interaction between R. microsporus and P. aeruginosa N1 was compared using monoculture and co-culture methods, with further analysis using liquid chromatography mass spectrophotometry (LC-MS). Results: Based on the LC-MS analysis, quinolone, phenazine and rhamnolipid compounds were the main substances produced during the interaction between species. There was a slight difference between the detected compounds produced in monocultures and those produced in co-culture. Specifically, 2-nonyl-4-hydroxyquinoline N-oxide and monorhamnolipid compounds were detected only in the P. aeruginosa N1 monocultures. On the other hand, the compounds 2-heptyl-4-quinolone, 1-hydroxy-2-[(2E)-2-nonen-1-yl]-4 (1H)-quinolinone and 2-nonyl-3-hydroxy-4-quinolone, were detected only in co-culture. Main finding: Quinolone, phenazine and rhamnolipid were the main compounds produced during the inhibitory interaction between P. aeruginosa N1 and R. microsporus. Furthermore, these compounds played an important role in improving antagonism against R. microsporus. However, this may regulate differently in each strain.

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Section: Isolation and Identification Of Associated Bacteria Causing ...mentioning

confidence: 99%

Phenazine and quinolone are main compounds produced by Pseudomonas aeruginosa N1 during inhibitory interaction with Rhizopus microsporus

2023

ANRES

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“…Lately, information about two other endosymbionts of Rhizopus microsporus has been published: Ralstonia pickettii as an facilitator of fungal invasion during infection ( Itabangi et al, 2019 ) and Stenotrophomonas sp. whose role has not yet been established ( Virgianti and Natalia, 2020 ). The activity of endohyphal and fungi-associated bacteria involves changes in lipid metabolism ( Lumini et al, 2007 ; Lastovetsky et al, 2016 ; Uehling et al, 2017 ) changes in morphology of the fungus ( Lumini et al, 2007 ; Salvioli et al, 2016 ; Uehling et al, 2017 ) control the hosts’ sexual ( Mondo et al, 2017 ), and asexual reproduction ( Partida-Martinez et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolic Potential, Ecology and Presence of Associated Bacteria Is Reflected in Genomic Diversity of Mucoromycotina

et al. 2021

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Mucoromycotina are often considered mainly in pathogenic context but their biology remains understudied. We describe the genomes of six Mucoromycotina fungi representing distant saprotrophic lineages within the subphylum (i.e., Umbelopsidales and Mucorales). We selected two Umbelopsis isolates from soil (i.e., U. isabellina, U. vinacea), two soil-derived Mucor isolates (i.e., M. circinatus, M. plumbeus), and two Mucorales representatives with extended proteolytic activity (i.e., Thamnidium elegans and Mucor saturninus). We complement computational genome annotation with experimental characteristics of their digestive capabilities, cell wall carbohydrate composition, and extensive total lipid profiles. These traits inferred from genome composition, e.g., in terms of identified encoded enzymes, are in accordance with experimental results. Finally, we link the presence of associated bacteria with observed characteristics. Thamnidium elegans genome harbors an additional, complete genome of an associated bacterium classified to Paenibacillus sp. This fungus displays multiple altered traits compared to the remaining isolates, regardless of their evolutionary distance. For instance, it has expanded carbon assimilation capabilities, e.g., efficiently degrades carboxylic acids, and has a higher diacylglycerol:triacylglycerol ratio and skewed phospholipid composition which suggests a more rigid cellular membrane. The bacterium can complement the host enzymatic capabilities, alter the fungal metabolism, cell membrane composition but does not change the composition of the cell wall of the fungus. Comparison of early-diverging Umbelopsidales with evolutionary younger Mucorales points at several subtle differences particularly in their carbon source preferences and encoded carbohydrate repertoire. Nevertheless, all tested Mucoromycotina share features including the ability to produce 18:3 gamma-linoleic acid, use TAG as the storage lipid and have fucose as a cell wall component.

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“…Lately, information about two other endosymbionts of Rhizopus microsporus has been published: Ralstonia pickettii as an enabler of fungal invasion during infection [29] and Stenotrophomonas sp. whose role has not yet been established [30]. The activity of endohyphal and fungi-associated bacteria involve (but is not limited to) change in lipid metabolism [31–33], change in morphology of the fungus [31,32,34], control of the hosts’ sexual [35], and asexual reproduction [36].…”

Section: Introductionmentioning

confidence: 99%

Metabolic potential, ecology and presence of endohyphal bacteria is reflected in genomic diversity of Mucoromycotina

Muszewska

Okrasińska

Steczkiewicz

et al. 2020

Preprint

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We describe the genomes of six Mucoromycotina fungi representing distant saprotrophic lineages within the subphylum (i.e. Umbelopsidales and Mucorales). We selected two Umbelopsis isolates from soil (i.e. U. isabellina, U. vinacea), two soil-derived Mucor isolates (i.e. M. circinatus, M. plumbeus), and two Mucorales representatives with extended proteolytic activity (i.e. Thamnidium elegans and Mucor saturninus). We complement genome analyses with a description of their digestive capabilities, their cell wall carbohydrate composition, and total lipid profiles. Finally, we link the presence of endohyphal bacteria with observed characteristics.One of the genomes, Thamnidium elegans, harbours a complete genome of an associated bacterium classified to Paenibacillus sp. This fungus displays multiple altered traits compared to remaining isolates regardless of their evolutionary distance. T. elegans has expanded carbon assimilation capabilities particularly efficiently degrades carboxylic acids, has a higher diacylglycerol: triacylglycerol ratio and phospholipid composition suggesting a more rigid cellular membrane. Comparison of early-diverging Umbelopsidales with evolutionary younger Mucorales points at several differences particularly in their carbon source preferences and encoded carbohydrate repertoire. All tested Mucoromycotina shares features including the ability to produce 18:3 gamma-linoleic acid and fucose as a cell wall component.Author SummaryIn our paper, we report on the genomic sequences of six Mucoromycotina strains and an associated bacterium from Paenibacillus genus. Mucoromycotina are often studied in pathogenic context albeit their basic biology remains understudied. This manuscript expands on the collection of currently sequenced Mucorales and Umbelopsidales, including the first sequenced Thamnidium isolate, which was sequenced together with a Paenibacillus bacterium. The interaction with a bacterial partner alters the metabolism, cell membrane composition but not the exoskeleton of the fungus. The associated bacterium provided multiple enzymes that significantly expanded the digestive capabilities of the fungal host. Parallel sequencing and phenotyping of Mucorales and Umbelopsidales enabled us to look at the differences of both lineages within Mucoromycotina. We demonstrate that the predicted digestive capabilities are in line with experimental validation. Based on the cell wall composition data and genomic underpinnings of carbohydrate metabolism we were able to confirm the universal presence of fucose in Mucoromycotina cell walls. Fatty acid, phospholipid and acylglycerol composition support the usage of 18:3 gamma-linoleic acid as a chemotaxonomic marker of Mucoromycotina and corroborate TAG as a dominant storage lipid in these organisms.Genomic features, digestive capabilities, fatty acid composition differ between Mucorales and Ubelopsidales pointing at subtle but significant changes in the course of Mucoromycotina radiation.

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Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus

Cited by 4 publications

References 37 publications

Phenazine and quinolone are main compounds produced by Pseudomonas aeruginosa N1 during inhibitory interaction with Rhizopus microsporus

Phenazine and quinolone are main compounds produced by Pseudomonas aeruginosa N1 during inhibitory interaction with Rhizopus microsporus

Metabolic Potential, Ecology and Presence of Associated Bacteria Is Reflected in Genomic Diversity of Mucoromycotina

Metabolic potential, ecology and presence of endohyphal bacteria is reflected in genomic diversity of Mucoromycotina

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