Short Communication Cross-species transferability of eastern white pine (Pinus strobus) nuclear microsatellite markers to five Mexican white pines

Villalobos-Arámbula, Alma Rosa; Rosa, Jorge A. Pérez de la; Arias, Armando; Rajora, Om P.

doi:10.4238/2014.september.12.24

Cited by 10 publications

(6 citation statements)

References 15 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The development of SSR markers is still inefficient, laborious and costly, principally in organisms with large and complex genomes, such as conifers. A number of researches have highlighted the ability of cross amplification in Pinus species (Villalobos-Arámbula et al, 2014;Xu et al, 2013;Chauhan et al, 2010;Xiang-Xiang and Ji-sen, 2005;Echt et al, 1998). In the present study, the population genetic parameters for ten populations of P. kesiya were estimated through transspecific amplification of chloroplast microsatellite markers from P. thunberghii (Vendramin et al 1996) and P. sylvestris (Provan et al 1998).…”

Section: Genetic Diversity and Gene Flowmentioning

confidence: 82%

Microsatellite Analysis to Study Genetic Diversity in Khasi Pine (Pinus Kesiya Royle Ex. Gordon) Using Chloroplast SSR Markers

Chamling

Ginwal

2018

Silvae Genetica

View full text Add to dashboard Cite

Pinus kesiya (Khasi pine) is the principal pine species in northeast India having high commercial value. Chloroplast microsatellites (cpSSR) were used to study the genetic diversity and population genetic structure of 10 populations of P. kesiya covering entire natural range of distribution in India. A total of 33 primer pairs (cpSSRs) of P. thunberghii and P. sylvestris were tested in P. kesiya for their transferability, out of which 18 chloroplast primers showed positive amplification and 10 were found polymorphic. A total of 250 individuals from 10 different populations were genotyped using the selected 10 cpSSRs. When alleles at each of the 10 loci were jointly analysed a total of 36 size variants were discovered, which combined to designate 90 haplotypes among 250 individuals. None of the haplotype was found common among the populations as they were population specific. The cpSSR indicated that P. kesiya populations have maintained a moderately high genetic diversity (HT=0.638) which is typical in most coniferous species. However, the inter-population genetic diversity was higher than the intra population diversity and the genetic differentiation between populations was also found to be very high (FST=0.47). A Bayesian cluster analysis separated the populations into six clusters where most of the individuals were found in single population clusters with minor admixtures. The distribution of genetic diversity and sub structuring of P. kesiya reflect week pollen mediated gene flow due to geographic isolation and genetic drift. The study has revealed useful cpSSR markers for P. kesiya, which were lacking earlier and also added an insight into the state of Khasi pine forest in the region, which can be useful for the better management and future conservation programs.

show abstract

Section: Genetic Diversity and Gene Flowmentioning

confidence: 82%

Microsatellite Analysis to Study Genetic Diversity in Khasi Pine (Pinus Kesiya Royle Ex. Gordon) Using Chloroplast SSR Markers

Chamling

Ginwal

2018

Silvae Genetica

View full text Add to dashboard Cite

show abstract

“…Southwestern white pine is of both commercial and ecological value. Commercial products include timber, cellulose, resin and pulp (Farjon and Styles, 1997;Villalobos-Arámbula et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Morphological Differences in Pinus strobiformis Across Latitudinal and Elevational Gradients

et al. 2020

View full text Add to dashboard Cite

show abstract

“…White pines (genus Pinus , subgenus Strobus ) are common tree species in temperate forest ecosystems. These species provide wood, resin, and pulp as well as valuable ecological services, such as provision of wildlife habitat (Farjon and Styles, 1997 ; Richardson, 2000 ; Villalobos-Arámbula et al, 2014 ). White pine seeds are large and nutritious and provide a valuable food source for rodents, parrots and other birds (Samano and Tomback, 2003 ; Looney and Waring, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Assessment and Models of Insect Damage to Cones and Seeds of Pinus strobiformis in the Sierra Madre Occidental, Mexico

et al. 2021

View full text Add to dashboard Cite

Insect damage to cones and seeds has a strong impact on the regeneration of conifer forest ecosystems, with broader implications for ecological and economic services. Lack of control of insect populations can lead to important economic and environmental losses. Pinus strobiformis is the most widespread of the white pines in Mexico and is widely distributed throughout the mountains of northern Mexico. Relatively few studies have examined insect damage to the cones and seeds of these pines, especially in Mexico. In this study, we therefore analyzed insect damage to cones and seeds of P. strobiformis in Mexico by using X-ray and stereomicroscopic analysis. The specific objectives of the study were (a) to characterize insect damage by measuring external and internal cone traits, (b) to assess the health of seeds and cones of P. strobiformis in the Sierra Madre Occidental, Mexico, and (c) to estimate the relative importance of the effects of different environmental variables on cone and seed damage caused by insects. We found that 80% of P. strobiformis seeds and 100% of the tree populations studied had damage caused by insects. Most seeds were affected by Leptoglossus occidentalis, Tetyra bipunctata, Megastigmus albifrons, and the Lepidoptera complex (which includes Apolychrosis synchysis, Cydia latisigna, Eucosma bobana, and Dioryctria abietivorella). The cones of all tree populations were affected by some type of insect damage, with Lepidoptera causing most of the damage (72%), followed by Conophthorus ponderosae (15%), the hemipteran L. occidentalis (7%), and the wasp M. albifrons (6%). The proportion of incomplete seeds in P. strobiformis at the tree level, cone damage by M. albifrons and seed damage in L. occidentalis were associated with various climate and soil variables and with crown dieback. Thus, cone and seed insect damage can be severe and potentially impact seed production in P. strobiformis and the reforestation potential of the species. The study findings will enable managers to better identify insects that cause damage to cone and seeds. In addition, identification of factors associated with damage may be useful for predicting the levels of insect predation on seeds and cones.

show abstract

Short Communication Cross-species transferability of eastern white pine (Pinus strobus) nuclear microsatellite markers to five Mexican white pines

Cited by 10 publications

References 15 publications

Microsatellite Analysis to Study Genetic Diversity in Khasi Pine (Pinus Kesiya Royle Ex. Gordon) Using Chloroplast SSR Markers

Microsatellite Analysis to Study Genetic Diversity in Khasi Pine (Pinus Kesiya Royle Ex. Gordon) Using Chloroplast SSR Markers

Morphological Differences in Pinus strobiformis Across Latitudinal and Elevational Gradients

Assessment and Models of Insect Damage to Cones and Seeds of Pinus strobiformis in the Sierra Madre Occidental, Mexico

Contact Info

Product

Resources

About