Short Communication Comparison of methods to isolate DNA from Caesalpinia ferrea

Sousa, Camila Campêlo de; Gomes, Sulimary Oliveira; Lopes, Ângela Celis de Almeida; Gomes, Regina Lúcia Ferreira; Britto, Fábio Barros; Lima, P. S. da C.; Valente, Sérgio Emílio dos Santos

doi:10.4238/2014.june.16.7

Cited by 12 publications

(11 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, the use of a commercial kit for DNA extraction has advantages, including practicality, simplicity, and the lack of acid reagents such as chloroform (Bitencourt et al, 2007;Sousa et al, 2014). However, the main problem with these commercially available kits is their high cost per sample (Ahmed et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

“…However, obtaining pure DNA samples is a difficult process because the biochemical composition among plant species can be different. For this reason, several protocols have been developed and adapted to the current variation of the biochemical composition of plant tissues (Sousa et al, 2014;Viana et al, 2015). A good protocol should be simple, rapid, and efficient, providing adequate amounts of high-quality DNA suitable for molecular analysis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of eight methods to isolate genomic DNA from Hancornia speciosa

Almeida¹,

Luz²,

Martins³

et al. 2017

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Mangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, mangabeira is threatened by genetic erosion in Brazil. The characterization of the genetic diversity of plants can provide the basis for strategies to protect and conserve endangered populations, like mangabeira. This study aimed to compare eight DNA extraction methods in mangabeira because the key to success is the use of a pure genomic DNA for the characterization of genetic diversity in molecular biology techniques. The quality and concentration of DNA were revealed by agarose gel electrophoresis. Polymerase chain reaction amplifications were successfully by extractions using two commercial purification kits and by the method proposed by Khanuja et al. (1999), which produced sufficient genomic DNA of good quality from leaves of H. speciosa to perform techniques involving molecular biology. The protocol described by Khanuja et al. (1999) is less expensive when compared to the commercial purification kits.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of eight methods to isolate genomic DNA from Hancornia speciosa

Almeida¹,

Luz²,

Martins³

et al. 2017

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Caesalpinia ferrea, commonly known as "pau-ferro" or "jucá", is considered a forage crop as the trees are common fodder for cattle in extensive rearing conditions in the Northeast region of Brazil (Nascimento et al, 2002). Jucá has been economically explored providing wood for construction, besides its use in folk medicine (Lucena et al, 2007;Sousa et al, 2014). The root of C. ferrea is used as an antipyretic and the decoction of stem bark is used to treat diabetes and rheumatism (Gomes, 2003).…”

Section: Introductionmentioning

confidence: 99%

Research Article Genotoxicity of Brosimum gaudichaudii (Moraceae) and Caesalpinia ferrea (Fabaceae) in Astyanax sp. (Characidae) based on a comet assay

Sousa¹,

Campos²,

Duarte³

et al. 2018

Genet. Mol. Res.

View full text Add to dashboard Cite

The plants Brosimum gaudichaudii (inharé) and Caesalpinia ferrea (jucá) are widely distributed throughout Brazil and are considered medicinal. Inharé has been popularly used as a blood purifier, for the treatment of skin and vitiligo, while jucáis considered analgesic, anti-inflammatory and anticancer. These therapeutic properties have been attributed to phytochemicals such as coumarins, flavonoids and tannins. However, the mechanisms of action of most of these phytochemicals have not yet been fully elucidated and they may compromise human health. Consequently, the evaluation of the genotoxic effect of these plant extracts is fundamental for the determination of safe doses for human consumption. We evaluated the genotoxic effect of various concentrations of extracts of B. gaudichaudii and C. ferrea using comet assay of erythrocytes from Astyanax sp. exposed in vivo. In the comet assay indicated the tail migration of DNA increased ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 17 (4): gmr18100 M.J.B. Souza et al. 2significantly in the group of cells exposed to C. ferrea for various treatments and the olive tail movement exhibited a significantly higher extent of DNA damage, indicating the potential genotoxicity of the extract. On the other hand, it is premature to claima lack of genotoxic effect for B. gaudichauddi extracts since our experimental design was not able to rule out apotential effect of DNA damage as the concentration of 20mg/L seemed to increase the likelihood of genotoxicity. Thus, larger doses of B. gaudichauddi extracts should be tested in future studies of the kind. Our investigation provided valuable data for two species of plants widely used in folk medicine in different regions of Brazil. We recommend caution when using thses species for their medicinal properties.

show abstract

“…There are several different protocols for DNA extraction that enable researchers to focus on variations in the biochemical composition among plant species. These protocols yield DNA of sufficient quality and quantity to be used in plant molecular studies (Sousa et al, 2014). It is known that contamination of the isolated DNA by polysaccharides, phenols, and secondary compounds can damage the DNA, inhibit the action of restriction enzymes, and cause interference in migration patterns on gel electrophoresis (Romano and Brasileiro, 1999).…”

Section: Introductionmentioning

confidence: 99%

Comparison of eight methods of genomic DNA extraction from babassu

et al. 2015

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Babassu (Orbignya phalerata Martius) is one of the most important palms in Brazil because of the largest morphological variation, wide geographic distribution, and high socio-economic importance. The diversity present in babassu germplasm should be protected against loss to ensure their use with high productivity. Study of the available variability in populations of babassu is necessary to develop conservation strategies. The study of genetic variability can be conducted using molecular markers and many of these studies require significant quantity of high-quality DNA. The present study aimed to effect comparison among eight DNA extraction methods in case of O. phalerata. The quality and concentration of nucleic acids were analyzed by spectrophotometry and integrity of DNA was ascertained by agarose gel electrophoresis. The spectrophotometry revealed that some methods resulted in high levels of concentration of nucleic acids, 18003-18008 (2015) in which values of the ratio A 260/280 and A 260/230 were outside the range of purity. The agarose gel electrophoresis established the concentration and integrity of DNA. The methods of Murray and Thompson (1980) and Ferreira and Grattapaglia (1998) did not result in satisfactory quantities of DNA. Conversely, the method proposed by Khanuja et al. (1999) resulted in DNA of adequate quality and quantity that could be satisfactorily used for amplification reactions performed with two ISSR primers.

show abstract

Short Communication Comparison of methods to isolate DNA from Caesalpinia ferrea

Cited by 12 publications

References 10 publications

Comparison of eight methods to isolate genomic DNA from Hancornia speciosa

Comparison of eight methods to isolate genomic DNA from Hancornia speciosa

Research Article Genotoxicity of <i>Brosimum gaudichaudii</i> (Moraceae) and <i>Caesalpinia ferrea</i> (Fabaceae) in <i>Astyanax</i> sp. (Characidae) based on a comet assay

Comparison of eight methods of genomic DNA extraction from babassu

Contact Info

Product

Resources

About