Short communication: A novel method using immunomagnetic separation with a fluorescent nanobeads lateral flow assay for the rapid detection of low-concentration Escherichia coli O157:H7 in raw milk

Huang, Zhen; Chen, Xi; Xie, Quan-Yuan; Liu, Daofeng; Lai, Weihua

doi:10.3168/jds.2016-11780

Cited by 26 publications

(12 citation statements)

References 15 publications

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“…The test result is positive when both the test and control lines attain a red color. Although there are many reported LFAs for E. coli detection, most of them were prepared using various sizes of gold nanoparticles (GNPs) and capture reagents that need a strip reader and also have time-consuming steps, such as signal or enzyme enhancement, PCR amplification, complex conjugation procedures, fluorescence labeling (Huang et al, 2016), and further incubations (Cho et al, 2015;Song et al, 2016a;Aissa et al, 2017). Besides, recognition of E. coli is generally reported to detect it alone even in medium or buffer (Terao et al, 2013;Bruno, 2014;Suria et al, 2015).…”

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confidence: 99%

Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Çam

Öktem

2017

Turk J Biol

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IntroductionMany strains of Escherichia coli bacteria live in the gastrointestinal tracts of humans and animals. E. coli O157 was reported as a food pathogen after the hemorrhagic colitis outbreak of 1982 (Riley et al., 1983). O157:H7 is one of the most important E. coli strains transmitted from cattle/ animals to humans (Dorn and Angrick, 1991;Altekruse et al., 1997;Slutsker et al., 1998), either by contact, eating contaminated foods, drinking contaminated water, or passing from one person to another directly (Heiman et al., 2015). Infections of humans with E. coli O157:H7 can result in clinical issues like hemolytic uremic syndrome, acute nonbloody diarrhea, and thrombocytopenic thrombotic purpura. A comparison of the outbreaks of E. coli O157:H7 during 2003 and 2012 showed their abundance recently in the United States (Heiman et al., 2015). Hospitalizations and infections caused by E. coli O157 are still being seen, according to food safety news.Since pathogenic E. coli contaminates food and water easily (Liu and Li, 2002) and causes severe defects in humans and animals, its early detection is crucial for public health. Therefore, many kinds of detection platforms, like real-time PCR (

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confidence: 99%

Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Çam

Öktem

2017

Turk J Biol

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“…The authors claimed that the results of this method was 10 times more sensitive than the conventional LFIA strips and comparable to the traditional ELISA (Song et al, 2016). Huang, Cui, Xie, Liu, & Lai, 2016, reported a method of immunomagnetic separation coupled with a fluorescent nanobeads LFIA to establish a method for E. coli O157:H7 detection in raw milk (Huang et al, 2016). The pathogen E. coli O157:H7 was captured from milk sample by immunomagnetic nanobeads and then detected by a fluorescent nanobeads LFIA.…”

Section: Recent Advances On E Coli O157:h7 Detection By Improved Lfiamentioning

confidence: 99%

“…The pathogen E. coli O157:H7 was captured from milk sample by immunomagnetic nanobeads and then detected by a fluorescent nanobeads LFIA. Screening times, that included immunomagnetic separation and LFIA proceed, were 8, 7, 6, and 5 hr when 1, 5, 25, and 125 cfu of E. coli O157:H7 were inoculated into 225 ml of raw milk, respectively (Huang et al, 2016).…”

Section: Recent Advances On E Coli O157:h7 Detection By Improved Lfiamentioning

confidence: 99%

Review of recent advances in improved lateral flow immunoassay for the detection of pathogenic Escherichia coli O157:H7 in foods

Sun

Kuo

et al. 2020

Journal of Food Safety

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The incidence of foodborne diseases has continuingly increased over the years and resulted in public health problem globally. Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) is a human pathogen that causes diarrhea and hemorrhagic colitis. E. coli O157:H7 can be found in various foods. It is important to detect this foodborne pathogen to provide safe food supply. A lot of methods, for example, culture and PCR‐based test, used to detect foodborne pathogens are laborious and time consuming. Hence, a variety of methods have been developed for rapid, simple and reliable detection of E. coli O157:H7 as it is required in many food analyses. Lateral flow immunoassay (LFIA) are advantageous over conventional detection methods in terms of their rapidity and simplicity for end user, especially the LFIA can be developed as the strip test for on‐site point‐of‐care test (POCT) products. Gold nanoparticles (AuNPs; colloid gold) are the most commonly used labels in the LFIA for the visual analysis, however, there are still several limitations that restrict their applications of traditional LFIA. Therefore, recent reports on improved LFIA for E. coli O157:H7 detection in foods are continuously reported. This review intends to provide these recent advances in improved LFIA methods for the detection of E. coli O157:H7 in foods.

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“…Lateral flow immunoassay (LFIA) has been widely used in the field detection of foodborne pathogens due to its simplicity, rapidity (15 min), specificity, and low cost (Zhang et al, 2015;Huang et al, 2016;Jiang et al, 2016;Luo et al, 2017;Huang et al, 2018a;Chen et al, 2020). However, traditional LFIA using 20-to 40nm gold nanoparticles (AuNP) as labels suffers from low sensitivity because of the insufficient brightness of AuNP (Jiang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Integrated gold superparticles into lateral flow immunoassays for the rapid and sensitive detection of Escherichia coli O157:H7 in milk

Chen

Yuan

et al. 2020

Journal of Dairy Science

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Escherichia coli O157:H7 is a common harmful foodborne pathogen that can cause severe diseases at low infectious doses. Traditional lateral flow immunoassay (LFIA) for the rapid screening of E. coli O157:H7 in food suffers from low sensitivity due to its dependence on 20-to 40-nm gold nanoparticles (AuNP) with insufficient brightness as labels. To address this issue, we reported for the first time the successful synthesis of gold superparticles (GSP) by encapsulating numerous small AuNP into a polymer nanobead using an evaporation-induced self-assembly method. Results indicated that the resultant GSP exhibited remarkably enhanced absorbance compared with the most widely used 40 nm AuNP in LFIA. In addition, the absorbance of GSP could be easily tuned by varying GSP sizes. Under optimized conditions, we achieved a rapid and sensitive determination of E. coli O157:H7 in milk with a detection limit of 5.95 × 10 2 cfu/mL when using the GSP with a size of 342 nm as LFIA signal reporters, exhibiting improvement of approximately 32-fold relative to the conventional 40 nm AuNP-LFIA method. We further demonstrated the selectivity, accuracy, reliability, and practicality of the proposed GSP-LFIA strip. In summary, this work offers a new strategy for improving LFIA sensitivity using assembled GSP as markers and demonstrates huge potential in rapidly and sensitively detecting foodborne pathogens.

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Short communication: A novel method using immunomagnetic separation with a fluorescent nanobeads lateral flow assay for the rapid detection of low-concentration Escherichia coli O157:H7 in raw milk

Cited by 26 publications

References 15 publications

Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Review of recent advances in improved lateral flow immunoassay for the detection of pathogenic Escherichia coli O157:H7 in foods

Integrated gold superparticles into lateral flow immunoassays for the rapid and sensitive detection of Escherichia coli O157:H7 in milk

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