Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Çam, Dilek; Öktem, Hüseyin Avni

doi:10.3906/biy-1705-50

Cited by 17 publications

(9 citation statements)

References 48 publications

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“…Reinholt et al [17] showed that an electrospun polylactic acid-polyethylene glycol (PLA-PEG) mat without further advancements is comparable in use as a lateral flow assay to a classic nitrocellulose membrane (Figure 1). They demonstrated its use as a matrix in an antibody-based Escherichia coli immunoassay, achieving a limit of detection of 3.8 × 10 6 CFU/mL, which is comparable to those seen in the literature for nitrocellulose membranes [18,19].…”

Section: Randomly-aligned Nanowiresmentioning

confidence: 62%

Polymeric Nanowires for Diagnostic Applications

2019

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Polymer nanowire-related research has shown considerable progress over the last decade. The wide variety of materials and the multitude of well-established chemical modifications have made polymer nanowires interesting as a functional part of a diagnostic biosensing device. This review provides an overview of relevant publications addressing the needs for a nanowire-based sensor for biomolecules. Working our way towards the detection methods itself, we review different nanowire fabrication methods and materials. Especially for an electrical signal read-out, the nanowire should persist in a single-wire configuration with well-defined positioning. Thus, the possibility of the alignment of nanowires is discussed. While some fabrication methods immanently yield an aligned single wire, other methods result in disordered structures and have to be manipulated into the desired configuration.

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Section: Randomly-aligned Nanowiresmentioning

confidence: 62%

Polymeric Nanowires for Diagnostic Applications

2019

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“…If the test strip works correctly and it is positive, both the test and control lines are seen as red. If no colored capture lines or only a red color at the test line appears, the strip is invalid, and the test should be repeated [21,23,24] ( Figure 2).…”

Section: Lateral Flow Assaymentioning

confidence: 99%

“…The disadvantage of dipping the strip into bacterial media directly is that flow can be retarded on conjugate pad because of the media components. Thus, using the suitable running buffer, simple phosphate buffer saline (PBS), may enable conjugate to flow through the strip test, efficiently [21,27]. Third is the immobilization of capture reagents.…”

Section: Lateral Flow Assaymentioning

confidence: 99%

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Lateral Flow Assay for Salmonella Detection and Potential Reagents

Çam¹

2020

New Insight Into Brucella Infection and Foodborne Diseases

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Salmonella is among the very important pathogens threating human and animal health. It is a common food pathogen transmitted from animals to humans via contaminated food, drinking water, and air. It invades the intestinal tract of hosts and causes salmonellosis leading to death. S. enteritidis was the most common species accounted for all salmonellosis cases. S. typhimurium is also another significant species causing the serious cases worldwide. To ensure public health, early detection of pathogens is crucial. Lateral flow assay (LFA), immunochromatographic assay, is a simple and rapid diagnostic test kits used in various fields and can be developed by, aptamers, antibodies (Abs), and nucleic acids. They are also being continued to develop different capture reagents coming from the recombinant technology. It has many advantages such as having mature technology, market presence, low cost, easy to use for end users without education, and stable shelf life. Gold nanoparticles (GNPs) are the most commonly used labels in the LFAs for the naked-eye analysis. Therefore, Salmonella detection by LFA based on GNPs in a rapid and simple way is always open to be developed by new reagents and methods.

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“…In conclusion, an AuNPs‐based LFIA is sensitive, simple and rapid assay for E. coli O157:H7 detection because it can be visually analyzed, does not require the skilled personnel and the availability of cost effective and large scale LFIA strips production ability (Çam, 2019). However, AuNPs‐based LFIA still have a lot of restrictions with respect to target agents of detection and assay conditions of LFIA (Borse et al, 2020; Çam & Öktem, 2017; Peruski & Peruski, 2003). In spite of the widespread use of AuNPs as a label in LFIA, there are still many additional molecules and methods used on LFIA development.…”

Section: Techniques Of Lateral Flow Immunoassaymentioning

confidence: 99%

Review of recent advances in improved lateral flow immunoassay for the detection of pathogenic Escherichia coli O157:H7 in foods

Sun

Kuo

et al. 2020

Journal of Food Safety

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The incidence of foodborne diseases has continuingly increased over the years and resulted in public health problem globally. Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) is a human pathogen that causes diarrhea and hemorrhagic colitis. E. coli O157:H7 can be found in various foods. It is important to detect this foodborne pathogen to provide safe food supply. A lot of methods, for example, culture and PCR‐based test, used to detect foodborne pathogens are laborious and time consuming. Hence, a variety of methods have been developed for rapid, simple and reliable detection of E. coli O157:H7 as it is required in many food analyses. Lateral flow immunoassay (LFIA) are advantageous over conventional detection methods in terms of their rapidity and simplicity for end user, especially the LFIA can be developed as the strip test for on‐site point‐of‐care test (POCT) products. Gold nanoparticles (AuNPs; colloid gold) are the most commonly used labels in the LFIA for the visual analysis, however, there are still several limitations that restrict their applications of traditional LFIA. Therefore, recent reports on improved LFIA for E. coli O157:H7 detection in foods are continuously reported. This review intends to provide these recent advances in improved LFIA methods for the detection of E. coli O157:H7 in foods.

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Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Cited by 17 publications

References 48 publications

Polymeric Nanowires for Diagnostic Applications

Polymeric Nanowires for Diagnostic Applications

Lateral Flow Assay for Salmonella Detection and Potential Reagents

Review of recent advances in improved lateral flow immunoassay for the detection of pathogenic Escherichia coli O157:H7 in foods

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