Short chain fatty acids (SCFAs) improves TNBS-induced colitis in zebrafish

Fénero, Camila Morales; Amaral, Mariana Abrantes do; Xavier, Izabella Karina; Padovani, Bárbara Nunes; Paredes, Laís Cavalieri; Takiishi, Tatiana; Lopes-Ferreira, Mônica; Lima, Carla; Colombo, Alicia; Câmara, Niels Olsen Saraiva

doi:10.1016/j.crimmu.2021.08.003

Cited by 17 publications

(13 citation statements)

References 133 publications

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“…These compact arrays of CLC droplets elicit similar optical responses when rehydrated with an amphiphile-containing sample, with the response time being as short as seconds for higher amphiphile concentrations. Lastly, we demonstrate proof-of-principle in vivo testing by gavaging live zebrafish larvae with the droplets, showing the potential of CLC droplets to detect amphiphiles within the intestine such as, for example, products of microbial fermentation 39,40 or metabolites that may be indicative of microbial dysbiosis or inflammatory processes.…”

Section: ■ Introductionmentioning

confidence: 90%

Designing Biological Microsensors with Chiral Nematic Liquid Crystal Droplets

Honaker

Chen

Dautzenberg

et al. 2022

ACS Appl. Mater. Interfaces

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Biosensing using liquid crystals has a tremendous potential by coupling the high degree of sensitivity of their alignment to their surroundings with clear optical feedback. Many existing setups use birefringence of nematic liquid crystals, which severely limits straightforward and frugal implementation into a sensing platform due to the sophisticated optical set-ups required. In this work, we instead utilize chiral nematic liquid crystal microdroplets, which show strongly reflected structural color, as sensing platforms for surface active agents. We systematically quantify the optical response of closely related biological amphiphiles and find unique optical signatures for each species. We detect signatures across a wide range of concentrations (from micromolar to millimolar), with fast response times (from seconds to minutes). The striking optical response is a function of the adsorption of surfactants in a nonhomogeneous manner and the topology of the chiral nematic liquid crystal orientation at the interface requiring a scattering, multidomain structure. We show that the surface interactions, in particular, the surface packing density, to be a function of both headgroup and tail and thus unique to each surfactant species. We show lab-ona-chip capability of our method by drying droplets in high-density two-dimensional arrays and simply hydrating the chip to detect dissolved analytes. Finally, we show proof-of-principle in vivo biosensing in the healthy as well as inflamed intestinal tracts of live zebrafish larvae, demonstrating CLC droplets show a clear optical response specifically when exposed to the gut environment rich in amphiphiles. Our unique approach shows clear potential in developing on-site detection platforms and detecting biological amphiphiles in living organisms.

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Section: ■ Introductionmentioning

confidence: 90%

Designing Biological Microsensors with Chiral Nematic Liquid Crystal Droplets

Honaker

Chen

Dautzenberg

et al. 2022

ACS Appl. Mater. Interfaces

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“…Zebrafish larvae from AB and Tg (mpx: GFP) strains were obtained by natural spawn, collected in 1X E3 medium (5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl 2 , 0.33 mM MgSO 4 , pH 7.2) and kept in Petri dishes at 28 °C incubator with 14/10 dark-light cycle with daily medium changes until 3 dpf. 25 , 26 The zebrafish (3 dpf) were placed in a 6-well plate (30 fish per well) and randomly divided into control group, model group, ATR group (7.8 μM) and clarithromycin group (167 μM). Except for the control group, the other groups were first treated with TNBS (125 μg/mL) at 28 °C for 48 h. The total quantity of TNBS to be used was prepared in 1X E3 medium.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Except for the control group, the other groups were first treated with TNBS (125 μg/mL) at 28 °C for 48 h. The total quantity of TNBS to be used was prepared in 1X E3 medium. After that, TNBS was removed, and H. pylori (5 × 10 7 CFU/mL) was added to all groups to establish a gastrointestinal mucosal injury model in zebrafish, and treated at 28 °C for 24 h. 26,27 H. pylori Culture H. pylori non-resistant strain GDMCC1.1820 was purchased from Guangdong Microbial Culture Collection Center. The strain was stored at −80 °C in brain heart infusion containing 30% glycerol.…”

Section: Establishment Of Gastrointestinal Mucosal Injury Model In Ze...mentioning

confidence: 99%

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Atractylone Alleviates Ethanol-Induced Gastric Ulcer in Rat with Altered Gut Microbiota and Metabolites

Li¹,

Du²,

Wang³

et al. 2022

JIR

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Background Gastric ulcer (GU) is the most common multifactor gastrointestinal disorder affecting millions of people worldwide. There is evidence that gut microbiota is closely related to the development of GU. Atractylone (ATR) has been reported to possess potential biological activities, but research on ATR alleviating GU injury is unprecedented. Methods Helicobacter pylori ( H. pylori )-induced GU model in zebrafish and ethanol-induced acute GU model in rat were established to evaluate the anti-inflammatory and ulcer inhibitory effects of ATR. Then, 16S rRNA sequencing and metabolomics analysis were performed to investigate the effect of ATR on the microbiota and metabolites in rat feces and their correlation. Results Therapeutically, ATR inhibited H. pylori -induced gastric mucosal injury in zebrafish. In the ulceration model of rat, ATR mitigated the gastric lesions damage caused by ethanol, decreased the ulcer area, and reduced the production of inflammatory factors. Additionally, ATR alleviated the gastric oxidative stress injury by increasing the activity of superoxide dismutase (SOD) and decreasing the level of malondialdehyde (MDA). Furthermore, ATR played a positive role in relieving ulcer through reshaping gut microbiota composition including Parabacteroides and Bacteroides and regulating the levels of metabolites including amino acids, short-chain fatty acids (SCFAs), and bile acids. Conclusion Our work sheded light on the mechanism of ATR treating GU from the perspective of the gut microbiota and explored the correlation between gut microbiota, metabolites, and host phenotype.

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“…Many factors regulate SCFA production, including bacterial communities in the gut, aging, and dietary fibers ( Macfarlane and Macfarlane, 2003 ; Ran An et al, 2019 ; Mizuno et al, 2020 ). Treatment with exogenous SCFAs and increasing endogenous SCFAs have been proposed to reduce inflammation in UC ( Vernia, 2007 ; Couto et al, 2020 ; Morales Fénero et al, 2021 ; Xiao et al, 2021 ). Among various therapies, herbal medicine has exhibited potential anti-inflammatory effects by regulating gut microbiota diversity and SCFA production ( Feng et al, 2018 ; Xuedong An et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Xuedan Sustained Release Pellets Ameliorate Dextran Sulfate Sodium–Induced Ulcerative Colitis in Rats by Targeting Gut Microbiota and MAPK Signaling Pathways

et al. 2022

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Cucurbitacins have a variety of bioactivities, such as anticancer, anti-inflammatory, antidepressant-like, and antiviral effects, but their pharmacological effect in ulcerative colitis (UC) has not been reported until now. Thus, this study aims to investigate the preventive effects of Xuedan sustained release pellets (XSPs) on UC rats and the underlying mechanisms. XSPs were prepared by extracting cucurbitacins from Hemsleya. Experimental UC rats were induced by the intake of 4% dextran sulfate sodium (DSS) for a week and treated with different doses of XSP (0.95, 1.90, and 3.8 mg/kg). The body weight, colon length, disease activity index (DAI), and histological changes of colonic tissue were measured. In addition, the expressions of pro-inflammatory cytokines were detected by using the enzyme-linked immunosorbent assay. Pathways involved in the intestinal inflammation were targeted by RNA-sequencing. Moreover, the changes of gut microbial diversity and composition were analyzed by the 16SrNA analysis and the contents of short-chain fatty acids (SCFAs) were detected by GC-MS. The results revealed that XSP intervention greatly restored the weight loss and colonic shortening (p < 0.05) and reduced the raised DAI scores, myeloperoxidase, and nitric oxide activities in UC in rats (p < 0.05). XSP administration also downregulated the protein levels of pro-inflammatory factors IL-1β, IL-6, and TNF-α. Notably, it was found that XSP considerably suppressed the activation of the MAPK signaling pathway. In addition, XSP treatment improved the balance of gut microbiota that was disturbed by DSS. The beneficial bacteria Lachnospiraceae_NK4A136 group and Lactobacillus at the genus level significantly increased in the XSP group, which had decreased with the use of DSS (p < 0.05). Pathogenic bacteria including Escherichia–Shigella and Bacteroides in UC in rats were reduced by XSP intervention. Furthermore, XSP significantly elevated the production of SCFAs in UC in rats (p < 0.05). These alterations in inflammatory status were accompanied with changes in gut microbiota diversity and SCFA production. In conclusion, XSP exhibited protective effects against DSS-induced UC in rats. XSP treatment decreased inflammation via modulation of gut microbiota composition and SCFA production.

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Short chain fatty acids (SCFAs) improves TNBS-induced colitis in zebrafish

Cited by 17 publications

References 133 publications

Designing Biological Microsensors with Chiral Nematic Liquid Crystal Droplets

Designing Biological Microsensors with Chiral Nematic Liquid Crystal Droplets

Atractylone Alleviates Ethanol-Induced Gastric Ulcer in Rat with Altered Gut Microbiota and Metabolites

Xuedan Sustained Release Pellets Ameliorate Dextran Sulfate Sodium–Induced Ulcerative Colitis in Rats by Targeting Gut Microbiota and MAPK Signaling Pathways

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