Shoot Morphogenesis: Structure, Physiology, Biochemistry and Molecular Biology

Joy, Richard W.; Thorpe, Trevor A.

doi:10.1007/978-94-015-9253-6_7

Cited by 10 publications

(4 citation statements)

References 165 publications

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“…This variability has been interpreted in different ways. While Joy and Thorpe (1999) addressed this variability in the sense that endogenous concentrations, rather than exogenous ones, determine the organogenic outcome, Veltcheva et al (2005) considered that the physiological and molecular mechanism underlying this factor is still unknown. The significant BA-experiment interaction, which explained a major part of the total variability for bud and shoot formation at the apical end in our experiments (Table 1), may not be explained solely in terms of endogenous cytokinin concentrations.…”

Section: Discussionmentioning

confidence: 99%

Light cytokinin interactions in shoot formation in epicotyl cuttings of Troyer citrange cultured in vitro

Molina

Castello

García-Luis

et al. 2007

Plant Cell Tiss Organ Cult

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Section: Discussionmentioning

confidence: 99%

Light cytokinin interactions in shoot formation in epicotyl cuttings of Troyer citrange cultured in vitro

Molina

Castello

García-Luis

et al. 2007

Plant Cell Tiss Organ Cult

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“…In fact, Joy and Thorpe (1999) noted that, based on the gaseous nature of ethylene, which diffuses easily through the tissues, endogenous ethylene measured after it has passed through the cells could still affect them prior to being released. The researchers pointed out that there is no direct method for separating this causeand-effect relationship.…”

Section: Resultsmentioning

confidence: 97%

Involvement of ethylene in the rooting of seedling shoot cultures of Bixa orellana L.

Neto

Reis

Finger

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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Using seedlings derived from the shoot apex of annatto (Bixa orellana L. cv. Bico-de-Pato) we observed the rooting frequency of B. orellana, the number and length of roots and the rate of ethylene production during 30 d in culture. The rhizogenesis response was affected by auxins (NAA or IBA) and by both the ethylene biosynthesis precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and the inhibitor 2-aminoethoxyvinylglycine (AVG). Auxin supplementation to the medium resulted in root induction, ethylene production, and an undesirable callusing in the epidermal and cortical tissues. Irrespective of the presence of auxins, supplementing the medium with ACC promoted ethylene biosynthesis and callusing, which resulted in increased cell proliferation mainly in the cortical and vascular tissues, while the epidermis was mostly unaltered. In both ACC and auxin-supplemented medium, increased ethylene production and callusing occurred, suggesting a synergistic effect between these two responses. ACC was capable of inducing adventitious root formation, but the roots produced had a wrinkled appearance when compared to normal roots. Conversely, AVG reduced ethylene production and callusing, while the epidermis, cortex, and inner tissues remained unaltered, regardless of the presence of auxins. AVG was beneficial in these aspects, although its application led to a reduction in the number of roots and in the average root length. In conclusion, it was not possible to establish a direct relation between ethylene and rooting, but we hypothesize that, under the experimental conditions described, ethylene may enhance tissue sensitivity to auxin. However, ethylene did not seem essential to the rhizogenesis process in annatto.

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“…180). Molecular, physiological, and biochemical studies have allowed for an indepth understanding of cytodifferentiation, mainly tracheary element formation [181], organogenesis [182,183], and somatic embryogenesis [184][185][186].…”

Section: The Presentmentioning

confidence: 99%

History of plant tissue culture

Thorpe

2007

Mol Biotechnol

Self Cite

240

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Plant tissue culture, or the aseptic culture of cells, tissues, organs, and their components under defined physical and chemical conditions in vitro, is an important tool in both basic and applied studies as well as in commercial application. It owes its origin to the ideas of the German scientist, Haberlandt, at the begining of the 20th century. The early studies led to root cultures, embryo cultures, and the first true callus/tissue cultures. The period between the 1940s and the 1960s was marked by the development of new techniques and the improvement of those that were already in use. It was the availability of these techniques that led to the application of tissue culture to five broad areas, namely, cell behavior (including cytology, nutrition, metabolism, morphogenesis, embryogenesis, and pathology), plant modification and improvement, pathogen-free plants and germplasm storage, clonal propagation, and product (mainly secondary metabolite) formation, starting in the mid-1960s. The 1990s saw continued expansion in the application of the in vitro technologies to an increasing number of plant species. Cell cultures have remained an important tool in the study of basic areas of plant biology and biochemistry and have assumed major significance in studies in molecular biology and agricultural biotechnology. The historical development of these in vitro technologies and their applications are the focus of this chapter.

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Shoot Morphogenesis: Structure, Physiology, Biochemistry and Molecular Biology

Cited by 10 publications

References 165 publications

Light cytokinin interactions in shoot formation in epicotyl cuttings of Troyer citrange cultured in vitro

Light cytokinin interactions in shoot formation in epicotyl cuttings of Troyer citrange cultured in vitro

Involvement of ethylene in the rooting of seedling shoot cultures of Bixa orellana L.

History of plant tissue culture

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