Light cytokinin interactions in shoot formation in epicotyl cuttings of Troyer citrange cultured in vitro

Molina, Rosa Victoria; Castello, S; García-Luis, Amparo; Guardiola, J. L.

doi:10.1007/s11240-007-9221-8

Cited by 19 publications

(18 citation statements)

References 18 publications

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“…When internodal segments were used as explant, the development of adventitious shoots was detected in 62% of the explants culture in culture media supplemented with 2.5 mg L -1 of BAP. Comparing this numbers with values of explant responsiveness obtained for other genotypes, such as 95% for Troyer and Carrizo citrange (MOREIRA-DIAS et al, 2001;BORDÓN et al, 2000), 90% for grapefruit (COSTA et al, 2004), and 96% for C. aurantifolia (PÉREZ-MOLPHE-BALCH; OCHOA-ALEJO, 1997) Rangpur lime can be considered a recalcitrant genotype.…”

Section: Introductionmentioning

confidence: 60%

“…Considering BAP culture media supplementation, contrasting with other citrus cultivars (GARCÍA-LUIS et al, 1999;MOREIRA-DIAS et al, 2001;MOURA et al, 2001) the presence of cytokinin is not essential for adventitious bud development. The percentage of responsive explants was stimulated with BAP concentrations up to 0.55 mg L -1 (Figure 2).…”

Section: Resultsmentioning

confidence: 96%

“…The success in in vitro citrus culture is related to genotype (KHAN et al, 2009), explant type (TAVANO et al, 2009), culture media composition (COSTA et al, 2004;SILVA et al, 2008), and incubation conditions (MOLINA et al, 2007). Most citrus in vitro organogenesis protocols are based on the culture of young tissue, mainly epicotyls and intermodal segments (GARCIA-LUIS et al, 1999;MOURA et al, 2001) cultivated in culture media supplemented with cytokinin (BORDÓN et al, 2000;ALMEIDA et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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In vitro organogenesis of rangpur lime

et al. 2012

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-Rangpur lime (Citrus limonia Osbeck) in vitro organogenesis was studied based on explant type and cytokinin culture media supplementation. Four explants types collected from epicotyl or hypocotyl regions of in vitro germinated seedlings were evaluated. The epicotyl-derived explants consisted of epicotyl segments and the hypocotyl-derived explants consisted of the entire hypocotyl segment, the hypocotyl segment attached to a cotyledon fragment, and the hypocotyl segment divided longitudinally. The explants were cultured on EME culture medium supplemented with benzylaminopurine (0, 0.5, 1.0, or 1.5 mg L -1 ). The evaluation was performed after 6 weeks. Best results considering both the explant responsiveness and number of shoots developed per explants were obtained when epicotyl segments-derived explants were evaluated. Considering the explant responsiveness of hypocotyl segments-derived explants no difference was detected between the entire hypocotyl segment and the hypocotyl segment attached to a cotyledon fragment. Moreover, the percentage of responsive explants decreased when hypocotyl segments divided longitudinally were tested. No difference was detected for the number of shoots developed per explant considering the three types of hypocotyl-derived explants. Culture media supplementation with BAP was not essential for Rangpur lime in vitro organogenesis. However, adventitious shoot development was stimulated in concentrations between 0.5 -1.0 mg L -1 . Index terms: adventitious shoot; Citrus limonia; tissue culture. ORGANOGÊNESE IN VITRO DE LIMÃO CRAVORESUMO -A organogênese in vitro de limão Cravo (Citrus limonia Osbeck) foi estudada em função do tipo de explante e da suplementação do meio de cultura com citocinina. Foram avaliados quatro tipos de explantes coletados da região do epicótilo e do hipocótilo de plântulas germinadas in vitro. Os explantes consistiram em segmentos de epicótilo, segmentos de hipocótilo, segmentos de hipocótilo ligado a um fragmento do cotilédone e segmentos de hipocótilo cortado longitudinalmente. Os explantes foram cultivados em meio de cultura EME suplementado com benzilaminopurina (0; 0,5; 1,0 e 1,5 mg L -1 ). As avaliações foram realizadas após seis semanas de incubação, e os melhores resultados considerando-se tanto a porcentagem de explantes responsivos como o número de gemas desenvolvidas por explante foram obtidos com o uso de segmentos de epicótilo como explante. Considerando os explantes derivados da região do hipocótilo, não foi detectada diferença na porcentagem de explantes responsivos com o uso de segmentos de hipocótilo ou de segmentos de hipocótilo ligado a um fragmento do cotilédone. A porcentagem de explantes responsivos diminuiu com o uso de segmentos de hipocótilo cortado longitudinalmente como explante. Não foi detectada nenhuma diferença no número de gemas adventícias desenvolvidas por explante considerando-se os três tipos de explantes derivados da região do hipocótilo. A suplementação do meio de cultura com BAP não é essencial para a organogênese ...

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Section: Introductionmentioning

confidence: 60%

Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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In vitro organogenesis of rangpur lime

et al. 2012

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“…Similarly, it was evidenced by Molina et al (2007) that zeatin, instead of kinetin, was most effective in shoot formation of Troyer citrange. However, the developmental response of the explants to exogenous hormones is related to the hormone concentration in the medium.…”

Section: Shoot Induction From In Vitro Stem Explantsmentioning

confidence: 75%

Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata

Ling

Tan

Hussein

2013

J. Zhejiang Univ. Sci. B

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Abstract:Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs.

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“…The concentration of the cytokinin used had a significant effect only on the organogenesis percentage achieved (p < 0.01), as every cytokinin tested proved to be more efficient at its lowest concentration. The inhibitory effect of high cytokinin concentration (especially that of BA) has been reported with other citrus species too (Moreira-Dias et al, 2000;Molina et al, 2007;Cervera et al, 2008). The control treatment resulted in nearly 5% successful organogenesis.…”

Section: -Cppu Enhances Sour Orange Epicotyl Explant Direct Shoot Ormentioning

confidence: 70%

Short communication. N-(2-chloro-4-pyridyl)-N-phenylurea(4-CPPU) enhances in vitro direct shoot organogenesis of Citrus aurantium L. epicotyl segments compared to other commonly used cytokinins

Roussos¹,

Dimitriou²,

Voloudakis³

2011

Span. j. agric. res.

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The effect of three concentrations of five different cytokinins, i.e. 6-benzylamino purine, 2-isopentyl adenine, kinetin (Kin), thidiazuron and N-(2-chloro-4-pyridyl)-N-phenylurea (4-CPPU), was evaluated on the in vitro direct shoot organogenesis of epicotyl explants of sour orange (Citrus aurantium L.). The basal medium used was that of Murashige and Tucker and epicotyl explants were incubated in medium supplemented with the prementioned cytokinins for 45 days. The addition of Kin and 4-CPPU in the medium enhanced the direct shoot organogenesis of sour orange epicotyl segments. The concentration of each of these two cytokinins which gave the best results, was combined with indole-3-acetic acid (IAA) or α-naphthalene acetic acid (α-NAA) at concentrations ranging from 0. presented equally high organogenesis percentages (91.7%). The incubation of the produced shoots, in medium supplemented with either indole-3-butyric acid or α-NAA resulted in high rooting percentages (up to 90%) and the rooted explants were successfully acclimatized under mist (85%). Although 4-CPPU has been used in in vitro culture of various species, this is the first report on its use in the direct shoot organogenesis of citrus species and could be of great value in citrus genetic transformation protocols using epicotyl segments, since this cytokinin resulted in the absolute organogenesis percentage. Additional key words: 2-isopentyl adenine; 6-benzylamino purine; explants; kinetin; sour orange; thidiazuron. Resumen Comunicación corta. El N-(2-cloro-4-piridil)-N-fenilurea (4 CPPU) mejora la regeneración in vitro vía organogénesis directa de segmentos de epicótilo de Citrus aurantium L. en comparación con otras citoquininas de uso comúnSe evaluó el efecto de tres concentraciones de cinco diferentes citoquininas [6-bencilaminopurina; 2-isopentil adenina; kinetina (Kin), tidiazurón y N-(2-cloro-4-piridil)-N-fenilurea (4-CPPU)], en la organogénesis directa in vitro de explantes de epicótilo de naranjo amargo (Citrus aurantium L.). Se incubaron durante 45 días explantes de epicótilo en medio Murashige y Tucker suplementado con las cinco citoquininas. La adición de Kin y 4-CPPU en el medio aumentó la organogénesis directa de segmentos de epicótilo de naranjo amargo. Se combinó la concentración que dio mejores resultados de cada uno de estas dos citoquininas con ácido indol-3-acético (IAA) o con ácido α-naftaleno acético (α-NAA) en concentraciones desde 0,01 hasta 0,2 mg L -1 . La inclusión de IAA a 0,2 mg L -1 en el medio con 4-CPPU a 0,05 mg L -1 resultó en un 100% de éxito en la organogénesis directa, mientras que la combinación de Kin a 0,25 mg L -1 con IAA o α-NAA, ambas a 0,01 mg L -1 , resultó en un 91,7% de organogénesis. La incubación de los

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Light cytokinin interactions in shoot formation in epicotyl cuttings of Troyer citrange cultured in vitro

Cited by 19 publications

References 18 publications

In vitro organogenesis of rangpur lime

In vitro organogenesis of rangpur lime

Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata

Short communication. N-(2-chloro-4-pyridyl)-N-phenylurea(4-CPPU) enhances in vitro direct shoot organogenesis of Citrus aurantium L. epicotyl segments compared to other commonly used cytokinins

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