Shoot induction competence and callus determination in Populus deltoides

Coleman, Gary D.; Ernst, Stephen G.

doi:10.1016/0168-9452(90)90070-5

Cited by 29 publications

(10 citation statements)

References 17 publications

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“…During the formation of the initial callus, the tissue theoretically became competent to respond to the subsequent inducing signal (Christianson & Warnick 1983). Similar stages have been distinguished in several other morphogenic systems (Nicotiana, Smulders et al 1990; Populus, Coleman & Ernst 1990;Ammirato 1985). A somatic embryogenesis system, previously developed in alfalfa (Meijer & Brown 1987), was used to characterize the competence and induction stages in terms of their auxin and time requirements in an effort to understand the nature of competence.…”

Section: Introductionmentioning

confidence: 73%

Characterization of competence during induction of somatic embryogenesis in alfalfa tissue culture

Finstad

Brown

Joy

1993

Plant Cell Tiss Organ Cult

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Systematic manipulations of the culture protocol leading to somatic embryogenesis in alfalfa petiolederived callus were performed to study the competence phase during somatic embryogenesis in alfalfa. These demonstrated a requirement for the acquisition of competence prior to the induction of the embryogenesis pathway. Induction was triggered by a number of synthetic auxins including 2,4-dichlorophenoxyacetic acid (2,4-D). Competence could be acquired in the presence of these auxins as well as phenylacetic acid (PAA), an auxin that did not induce embryogenesis. Different degrees of competence were apparently acquired by exposure to 2,4-D and PAA. Some degree of competence was acquired in the absence of auxin treatment. The current understanding of the concept of competence is discussed.

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Section: Introductionmentioning

confidence: 73%

Characterization of competence during induction of somatic embryogenesis in alfalfa tissue culture

Finstad

Brown

Joy

1993

Plant Cell Tiss Organ Cult

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“…In general, most of the regeneration experiments carried out on poplar species have been preferentially conducted on hybrid poplar clones from the section Populus because these are easily micropropagated in vitro and plant regeneration from different organs is rather easy to induce (Leplé et al 1999). In contrast, there have been very few publications reporting data on inter-or euramerican hybrids in vitro culture, since P. deltoides and its interspecific hybrids are known to be particularly recalcitrant to shoot regeneration (Sellmer et al 1989;Coleman and Ernst 1990).…”

Section: Introductionmentioning

confidence: 95%

Optimization of in vitro micropropagation and regeneration for Populus × interamericana and Populus × euramericana hybrids (P. deltoides, P. trichocarpa, and P. nigra)

2002

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A rapid and efficient micropropagation method has been established for six European poplar cultivars of economic interest -four Populus × interamericana and two Populus × euramericana. Using a three-step procedure, we were able to regenerate plantlets from callus and acclimate them within 4 months. In the first step, callogenesis was induced when explants were cultured for 25 days on culture medium supplemented with 10 µM α-naphthaleneacetic acid and 5 µM N 6 (2-isopentenyl) adenine. Bud regeneration followed by shoot elongation was then obtained from callus tissue by combining the cytokinin-like compound thidiazuron with the surfactant Pluronic F-68 at concentrations adjusted for each cultivar. The usefulness of this procedure in the area of genetic engineering is discussed.Abbreviations 2iP: N 6 (2-isopentenyl)adenine · NAA: α-Naphthaleneacetic acid · TDZ: Thidiazuron · WPM: Woody plant medium Communicated by M. Beckert

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“…According to the study, nine Populus tremula clones were tested for direct regeneration, with the Dursunbey genotype giving the best results. Similarly, Coleman and Ernst (1990) showed that the adventitious shoot regeneration competence states of P. deltoides were genotype dependent. Rutledge and Douglas (1988) studied 12 commercial clones of the poplar in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…There are several studies on in vitro organogenesis with Populus spp. including P. deltoides or its hybrids (Rutledge and Douglas, 1988;Sellmer et al, 1989;Coleman and Ernst, 1990;Noël et al, 2002;Yadav et al, 2009). However, with several of the known Populus genotypes, it was found that they are hard to perpetuate and difficult to work with for in vitro culture studies (Yadav et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Direct and indirect plant regeneration from various explants of eastern cottonwood clones (Populus deltoides Bartram ex Marsh.) with tissue culture

Çavuşoğlu¹,

Ipekci-Altas²,

Bajroviç³

et al. 2011

Afr. J. Biotechnol.

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Shoot induction competence and callus determination in Populus deltoides

Cited by 29 publications

References 17 publications

Characterization of competence during induction of somatic embryogenesis in alfalfa tissue culture

Characterization of competence during induction of somatic embryogenesis in alfalfa tissue culture

Optimization of in vitro micropropagation and regeneration for Populus × interamericana and Populus × euramericana hybrids (P. deltoides, P. trichocarpa, and P. nigra)

Direct and indirect plant regeneration from various explants of eastern cottonwood clones (Populus deltoides Bartram ex Marsh.) with tissue culture

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