A rapid and efficient micropropagation method has been established for six European poplar cultivars of economic interest -four Populus × interamericana and two Populus × euramericana. Using a three-step procedure, we were able to regenerate plantlets from callus and acclimate them within 4 months. In the first step, callogenesis was induced when explants were cultured for 25 days on culture medium supplemented with 10 µM α-naphthaleneacetic acid and 5 µM N 6 (2-isopentenyl) adenine. Bud regeneration followed by shoot elongation was then obtained from callus tissue by combining the cytokinin-like compound thidiazuron with the surfactant Pluronic F-68 at concentrations adjusted for each cultivar. The usefulness of this procedure in the area of genetic engineering is discussed.Abbreviations 2iP: N 6 (2-isopentenyl)adenine · NAA: α-Naphthaleneacetic acid · TDZ: Thidiazuron · WPM: Woody plant medium Communicated by M. Beckert
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