Shoot Generation and Callus Induction of Dioscorea hispida Dennst by Different Plant Growth Hormones and Basal Media

Abstract

Dioscorea hispida Dennst produces tuber which possess valuable medicinal properties but unsustainable harvesting has led to its reduction. The plant propagates slowly because of its low tuber sprouting rate. In average, Dioscorea hispida Dennst tubers took approximately 60 d to break dormancy and sprout. Hence, callus culture is proposed as a possible efficient type of culture for manipulation of this species. In the present study, calli were induced from stem segments to evaluate callus culture potential of Dioscorea hispida Dennst. Results indicate that the combination of 1 mgL-1 naphthaleneacetic acid (NAA), 1 mgL-1 6- benzylaminopurine (BAP) and 0.5 mgL-1 2,4-dichlorophenoxyacetic acid (2, 4-D) in Gamborg (B5) medium improved callus multiplication and differentiation in the stem culture as opposed to those in Murashige and Skoog (MS) medium. The findings from the present study provide the basis of callus culture protocol for stem explant of Dioscorea hispida Dennst with B5 being the more effective basal medium.

Effects of Indole-3-Butyric Acid (IBA) and rooting media on rooting and survival of air layered wax apple (Syzygium samarangense) CV Jambu Madu

“…Thus, leading to rapid growth and development which contributed to higher leaf area of leaves (Akwatulira et al, 2011). Mahmod et al (2020) also reported that plant growth regulators application improved callus multiplication and differentiation in the stem culture of ubi gadong. Our study indicated that rooting hormone, rooting medium and their interaction produced significant effects on leaf chlorophyll content of wax apple air layers.…”

“…In this study, the rooting media and interaction of rooting media and hormones produced a significant effect on leaf area of rooted wax apple air layers. With root inducing hormone applied with rooting medium produced more roots in cuttings, thus absorbing more nutrients and water, and increased the leaf area stem cuttings (Stancato et al, 2003;Mahmod et al, 2020). The aeration capacity of the rooting media is accountable for improving metabolic activities, enhancing vegetative growth and survivability of air layers (Yeboah et al, 2009).…”