Shoot Formation from Explant Cultures of Fourteen Potato Cultivars and Studies of the Cytology and Morphology of Regenerated Plants

Wheeler, V. A.; Evans, N. E.; Foulger, D.; Webb, K. Judith; Karp, A.; Franklin, J. F.; Bright, S. W. J.

doi:10.1093/oxfordjournals.aob.a086907

Cited by 69 publications

(35 citation statements)

References 8 publications

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“…For instance, plants regenerated directly from shoot tips tend to maintain their original genetic make up. Adventitious shoots/embryos arising directly from explants ( VAN HARTEN et al 1981;WHEELER et al 1985) or through a callus phase, exhibit genetic variations (LARKIN and ScowcROFT 1981;ORTON 1980;EvANS 1989), which increase with time for which the tissue has been maintained in culture, before regenerating plants (NAGRAJAN and WALTON 1987). In cucurbits KIM et al (1988) reported that the regenerated plants of cucumber (Cucumis sativus) were tetraploid.…”

Section: Discussionmentioning

confidence: 99%

Chromosome variations in the plants regenerated from leaf explants ofCucumis meloL. cv. ‘Pusa sharbati’

1992

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SUMMARY -The present communication deals with the cytological analysis of plants regenerated in vitro from leaf explants of Cucumis melo L. cv. 'Pusa sharbati'. The regenerants were variously multiplied in vitro prior to studying their ploidy level. Cytological analysis revealed that plants raised through leaf culture were euploid and aneuploid at diploid, tetraploid and octaploid levels. The ploidy level of regenerants increased with increase in the period of shoot culture on shoot multiplication medium. Among regenerants tetraploids were more frequent than octaploids and diploids.

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Section: Discussionmentioning

confidence: 99%

Chromosome variations in the plants regenerated from leaf explants ofCucumis meloL. cv. ‘Pusa sharbati’

1992

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“…Successful in vitro plant regeneration has been achieved from explants of different organs and tissues of the potato (Solanum tuberosum L.) such as tuber discs (Lam, 1977;Bragdo-Aas, 1977: Jarret et al, 1980aKikuta & Okazawa, 1984: Wang & Hu, 1985Smolenskaya, 1992), shoots (Patrascu, 1981;Maroti et al, 1982;Wheeler et al, 1985), and leaves (Roest & Bokelmann, 1980;Webb et al, 1983;Hulme et al, 1992). Various kinds and combinations of growth regulators were used in these studies.…”

Section: Introductionmentioning

confidence: 99%

“…Auxins are used for callus production and culture, and cytokinins for adventitious shoot formation. These two stages have been integrated by using one culture medium containing a mixture of auxins and cytokinins (Lam, 1977;Jarret et al, 1980a,b;Smolenskaya, 1992), or may be separated by using two culture media, with auxins in the first to produce callus and cytokinins in the second medium to generate adventitious shoots (Bragdo-Aas, 1977;Mix & Sixin, 1983;Wheeler et al, 1985). In both cases, roots must then be initiated and grown from these shoots in a further culture stage.…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration from tuber discs of potato (Solanum tuberosum L.) using 6-benzylaminopurine (BAP)

Esna-Ashari

Villiers

1998

Potato Res

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SummaryShoots, roots and callus were formed from tuber discs of potato, cultivar Ddsirde, when grown in vitro on the basal medium of Murashige & Skoog (1962) (MS) supplemented with 2,4-D and/or BAP. Callus was formed in MS medium with 1 mg I -I BAP plus 0.5 mg 1 -I 2,4-D, callus and roots were formed in MS with 1 mg 1-1 BAP plus more than 0.5 mg 1 -I 2,4-D and shoots were formed directly on tuber discs cultured on MS medium with I mg 1 -I BAP without the addition of 2,4-D.Nodules produced at the explant surface after the 4th week increased in size following subculture onto the same medium (MS+BAP alone), and 2 to 6 shoots developed from each nodule. After 9 weeks total time in culture, these shoots were excised and transferred as cuttings to MS medium without growth regulators, after which roots developed and plantlets were formed.A histological study of the explants at the sites of nodule formation indicated that the shoots developed from meristematic zones initiated within small outgrowths of tissue similar to those occuring in adventive organogenesis but the presence of shoot and root meristems associated with the same axis suggests the formation of somatic embryos.

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“…Recent rapid developments 428 in technology may allow much more sophisticated and complex transgenic crops to be grown in the future (Vreugdenhil et al, 2007). One of the key requirements for an efficient transformation system is the ability to readily regenerate plants from an isolated explant, and in potato a number of strategies have been successfully employed for a wide range of germplasm (Wheeler et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Application of genetic engineering in potato breeding

Gorji¹,

Polgár

2010

Acta Agronomica Hungarica

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Potato breeding programmes worldwide are undergoing a period of rapid change. In order to be successful, breeders must adapt and incorporate the newest up-to-date techniques as they become available. Recent advances in biotechnology make it possible to develop and cultivate more and more sophisticated transgenic crops with multiple modified traits. Gene transfer methods can be used for a wide range of fundamental studies, contributing to a better understanding of the mechanisms of plant/pathogen interactions and the metabolic pathways in plants. Transgenic potato plants are being generated worldwide to investigate the impact of transgene expression on parameters as complex as yield. Historically, potato was one of the first successfully transformed crop plants. Nowadays, transgenic potatoes have been introduced into the food chain of people and animals in several countries. Some of the genetic modifications give potato plants increased resistance to biotic and abiotic environmental factors, while others lead to improved nutritional value, or cause the plants to produce proteins of the immune system of humans or animals or substances that may be used as vaccines in humans or veterinary medicine. The trend today is towards the generation of crops with output traits, e.g. modified starch or carotenoids, or the production of pharmaceuticals in tubers, whereas the early targets were input traits, e.g. herbicide resistance, pest or virus resistance. This review provides a summary of examples illustrating the versatility and applicability of transgenic biology in potato improvement.

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Shoot Formation from Explant Cultures of Fourteen Potato Cultivars and Studies of the Cytology and Morphology of Regenerated Plants

Cited by 69 publications

References 8 publications

Chromosome variations in the plants regenerated from leaf explants ofCucumis meloL. cv. ‘Pusa sharbati’

Chromosome variations in the plants regenerated from leaf explants ofCucumis meloL. cv. ‘Pusa sharbati’

Plant regeneration from tuber discs of potato (Solanum tuberosum L.) using 6-benzylaminopurine (BAP)

Application of genetic engineering in potato breeding

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