Shoot apical meristem: A sustainable explant for genetic transformation of cereal crops

Sticklen, Mariam B.; Oraby, Hesham F.

doi:10.1079/ivp2004616

Cited by 77 publications

(51 citation statements)

References 143 publications

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“…Explants derived from mature seeds are considered an excellent source material for biotechnological application due to easy storage and accessibility to large amounts of uniform quality explant material (Sudhakar et al, 2004). Shoot apical meristems have been used successfully in the regeneration systems in cereals as starting material to obtain stable transformation in barley, wheat, maize, sorghum and millet (Sticklena and Orabya, 2005). The use of shoot apex in regeneration is critical since it can divide to produce viable new organs such as leaves, stems and adventitious roots (Itoh et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Efficient plant regeneration protocol for finger millet [Eleusine coracana (L.) Gaertn.] via somatic embryogenesis

Ngetich¹,

Mweu²,

Ngugi³

et al. 2018

Afr. J. Biotechnol.

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In the present study, an efficient protocol for somatic embryogenesis and plant regeneration was established in six finger millet varieties (GBK-043137, GBK-043128, GBK-043124, GBK-043122, GBK-043094 and GBK-043050). Shoot tips from 3 days in vitro grown plants were inoculated on MS supplemented with various concentrations and combinations of α-naphthaleneacetic acid (NAA), 2,4-Dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP) and kinetin for callus induction and somatic embryogenesis. For shoot regeneration, somatic embryos were cultured on various concentrations of BAP, while root induction was done using different concentrations and combinations of NAA, kinetin, BAP and 2,4-D. Acclimatization of regenerated plants was tested using forest soil, cocopeat, manure, sand and fertilizer either singly or in combination. Best callus formation was achieved on 2.5 mg/l of 2,4-D and 1.5 mg/l BAP with a mean of 12.33±0.33 on variety GBK-043128 while shooting and rooting were best on 1.75 mg/l BAP with a mean of 25.07±0.64 and 1.0 BAP+0.25 NAA with a mean of 15.00±2.2, respectively. Best acclimatization was attained using soil, sand and fertilizer on GBK-043094. Plants regenerated were morphologically similar to in vivo plants with 97% survival rate. Moreover, they were fertile and able to set viable seeds. This efficient protocol has the potential for crop improvement and genomic studies.

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Section: Discussionmentioning

confidence: 99%

Efficient plant regeneration protocol for finger millet [Eleusine coracana (L.) Gaertn.] via somatic embryogenesis

Ngetich¹,

Mweu²,

Ngugi³

et al. 2018

Afr. J. Biotechnol.

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“…Shoot apices are used as explants in many cereals since they produce plantlets identical to their parents. Main steps involved in shoot apex culture are the separation of tissues surrounding the meristem followed by exposing the meristem by creating wounds [44]. Girijashankar et al, [14] used shoot apices of sorghum BTX623 genotype for cotransformation with a selectable marker (bar) and an insect resistant (Cry1AC) gene using plasmids pJS108 and pmpiCIcry1AC.…”

Section: Discussionmentioning

confidence: 99%

Development of transgenic Sorghum bicolor (L.) Moench resistant to the Chilo partellus (Swinhoe) through Agrobacterium-mediated transformation

Ignacimuthu¹,

Premkumar²

2014

Mol Biol Genet Eng

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“…In their efforts to improve plant transformation techniques, the researchers continued to attempt transformation studies using various in vivo (on planta) tissues and organs, such as the shoot apex, apical meristems and mature seeds (Chen and Dale, 1992;Sticklen and Oraby, 2005;Al-Abed et al, 2006;Risacher and Craze, 2012). These strategies are simple, time-and labor-effective and genotype-independent since they do not depend on tissue culture procedures.…”

Section: Introductionmentioning

confidence: 99%

Generation of Transgenic Maize by Two Germinating Seed Transformation Methods

XueLian¹,

Du²,

Yaoshan³

et al. 2015

IJAB

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Two germinating seed transformation methods i.e., scarifying and puncturing methods, were used to transfer the bar gene into an inbred maize line. We generated transgenic glufosinate-tolerant maize plants, and conducted a comparison of the two transformation methods. We used the plasmid pCAMBAR.CHI.11 as the donor and germinating maize seeds as the recipients. The germinating seeds were subjected to puncture or scarifying treatment after being soaked in water for 12 h. The injured seeds were co-cultivated with Agrobacterium for 24 h and then sown in the experimental plots. Putative transgenic maize plants were selected by basta tolerance screening and PCR detection, and were further confirmed by Southern blotting. Following glufosinate tolerance assay and molecular detection of transgenic lines of the transgenic seedling (T0), first generation of transgenic seedling (T1) and second generation of transgenic seedling (T2), we have confirmed that the bar gene was stably inherited and expressed. The PCR amplification result for T2 transgenic plants showed that the genetic segregation ratio of the bar gene followed the 3:1 ratio of a single dominant Mendelian factor. The study proved that both the germinating seed treatment approaches are rapid and simple plant transformation methods. In particular, the application of the puncture method can expand the tissue culture free transformation to dicotyledonous plants.

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Shoot apical meristem: A sustainable explant for genetic transformation of cereal crops

Cited by 77 publications

References 143 publications

Efficient plant regeneration protocol for finger millet [Eleusine coracana (L.) Gaertn.] via somatic embryogenesis

Efficient plant regeneration protocol for finger millet [Eleusine coracana (L.) Gaertn.] via somatic embryogenesis

Development of transgenic Sorghum bicolor (L.) Moench resistant to the Chilo partellus (Swinhoe) through Agrobacterium-mediated transformation

Generation of Transgenic Maize by Two Germinating Seed Transformation Methods

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