Development of transgenic Sorghum bicolor (L.) Moench resistant to the Chilo partellus (Swinhoe) through Agrobacterium-mediated transformation

Ignacimuthu, Savarimuthu; Premkumar, A.

doi:10.7243/2053-5767-2-1

Cited by 10 publications

(3 citation statements)

References 42 publications

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“…The gus reporter gene expression was examined histochemically to con rm the transient transformation of calli using the GUS assay as described by Ignacimuthu et al (2014) [13]. 400 µL of X-gluc in 1.5 mL eppendorf tubes containing callus samples from selection media was used for the GUS assay.…”

Section: Histochemical Gus Assaymentioning

confidence: 99%

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Invitro regeneration and standardization of Agrobacterium mediated transformation of immature embryo callus from Sweet sorghum

Yadala¹,

Bandi²,

Palle³

et al. 2022

Preprint

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Sweet sorghum is the present target and cheap source for biofuel production. Tissue culture recalcitrance and low levels of transformation reproducibility of protocols are main constrains for transgenic sweet sorghum development. Production of a transient transgenic sweet sorghum for the development of disease resistant transgenics was the aim of our studies. In this study we have developed an efficient regeneration and transformation system for in vitro culture of sweet sorghum from the immature embryo callus. Histological studies of sweet sorghum embryogenic calli revealed that the development of embryogenic shoots. Murashige and Skoog nutrient agar medium with different concentrations of 2mg/l 2,4-D and combination of 0.2 mg/l NAA and 2 mg/l Kinetin gave higher frequency of callus induction and shoot regeneration. Embryogenic callus was competent to accept the DNA with GV2600 strain. These tissues are susceptible to Agrobacterium mediated transformation carrying pCAMBIA1301 with gus gene construct as well as for shoot multiplication. For effective transformation, GV2600 strain carrying pCAMBIA1301 at 0.6 O.D. was found to be compatible in giving sweet sorghum transgenics. Molecular confirmation was done by Polymerase Chain Reaction and Southern blotting for the putative plants. The regenerated plantlets survived during acclimatization were growing similar to the normal plants. All the major parameters that affecting transformation frequency were optimized for the development of perfect sweet sorghum transgenics.

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Section: Histochemical Gus Assaymentioning

confidence: 99%

“…The regeneration and transformation frequency of sweet sorghum is relatively low compared to other sweet sorghum varieties [8][9][10]. But in certain sorghum varieties, high transformation frequency has been reported [11][12][13][14][15]. Zhao et al (2003) [16] for the rst time successfully developed transgenic sweet sorghum with gus gene.…”

Section: Introductionmentioning

confidence: 99%

Invitro regeneration and standardization of Agrobacterium mediated transformation of immature embryo callus from Sweet sorghum

Yadala¹,

Bandi²,

Palle³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…In addition to its principal uses as flour, in the preparation of porridge and unleavened bread, sorghum species are sources of fiber, fuel and secondary products and are also used in the alcohol industry (sweet sorghum) as they contain high amounts of starch. In addition, sorghum grain has enhanced protein quality that could contribute significantly to the nutritional value of the diets of people and livestock (Ignacimuthu and Premkumar, 2014). Sorghum has been considered as one of the most difficult plant species to manipulate through tissue culture (Manjula et al, 2000;Chandrakanth et al, 2002;Hagio, 2002;Harshavardhan et al, 2002;Jeoung et al, 2002;Visarada et al, 2003;Kishore et al, 2006;Gupta et al, 2006;Maheswari et al, 2006).…”

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confidence: 99%