Shiitake Culinary-Medicinal Mushroom, Lentinus edodes (Agaricomycetes), Supplementation Alters Gut Microbiome and Corrects Dyslipidemia in Rats

Anwar, Haseeb; Suchodolski, Jan S.; Ullah, Muhammad Irfan; Hussain, Ghulam; Shabbir, Muhammad Zubair; Mustafa, Imtiaz; Sohail, Muhammad Umar

doi:10.1615/intjmedmushrooms.2018029348

Cited by 19 publications

(9 citation statements)

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“…Cobas 6000 analyzer (Roche Diagnostics) was used for measuring enzymes, hormones, and metabolites, as described previously. 23 , 24 HOMA-IR (Homeostatic Model Assessment for Insulin Resistance) was calculated using following equation:…”

Section: Methodsmentioning

confidence: 99%

Dysbiosis of the Salivary Microbiome is Associated with Hypertension and Correlated with Metabolic Syndrome Biomarkers

Sohail¹,

Hedin²,

Al-Asmakh³

2021

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Section: Methodsmentioning

confidence: 99%

Dysbiosis of the Salivary Microbiome is Associated with Hypertension and Correlated with Metabolic Syndrome Biomarkers

Sohail¹,

Hedin²,

Al-Asmakh³

2021

DMSO

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“…Kit (FC-131-1002, Illumina Inc., San Diego, CA, USA). In brief, the V3-V4 region of the 16S rRNA gene was amplified using a 337F/805R primer pair [24], followed by an Illumina two-step amplification library preparation strategy [25]. Prepared libraries were cleaned and normalized using magnetic beads (Agencourt Ampure XP, Beckman Coulter, IN, USA).…”

Section: S Rrna Sequencingmentioning

confidence: 99%

Profiling the Oral Microbiome and Plasma Biochemistry of Obese Hyperglycemic Subjects in Qatar

et al. 2019

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The present study is designed to compare demographic characteristics, plasma biochemistry, and the oral microbiome in obese (N = 37) and lean control (N = 36) subjects enrolled at Qatar Biobank, Qatar. Plasma hormones, enzymes, and lipid profiles were analyzed at Hamad Medical Cooperation Diagnostic Laboratory. Saliva microbiome characterization was carried out by 16S rRNA amplicon sequencing using Illumina MiSeq platform. Obese subjects had higher testosterone and sex hormone-binding globulin (SHBG) concentrations compared to the control group. A negative association between BMI and testosterone (p < 0.001, r = −0.64) and SHBG (p < 0.001, r = −0.34) was observed. Irrespective of the study groups, the oral microbiome was predominantly occupied by Streptococcus, Prevotella, and Veillonella species. A generalized linear model revealed that the Firmicutes/Bacteroidetes ratio (2.25 ± 1.83 vs. 1.76 ± 0.58; corrected p-value = 0.04) was higher, and phylum Fusobacteria concentration (4.5 ± 3.0 vs. 6.2 ± 4.3; corrected p-value = 0.05) was low in the obese group compared with the control group. However, no differences in microbiome diversity were observed between the two groups as evaluated by alpha (Kruskal–Wallis p ≥ 0.78) and beta (PERMANOVA p = 0.37) diversity indexes. Certain bacterial phyla (Acidobacteria, Bacteroidetes, Fusobacteria, Proteobacteria, Spirochaetes, and Firmicutes/Bacteroidetes) were positively associated (p = 0.05, r ≤ +0.5) with estradiol, fast food consumption, creatinine, breastfed during infancy, triglycerides, and thyroid-stimulating hormone concentrations. In conclusion, no differences in oral microbiome diversity were observed between the studied groups. However, the Firmicutes/Bacteroidetes ratio, a recognized obesogenic microbiome trait, was higher in the obese subjects. Further studies are warranted to confirm these findings in a larger cohort.

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“…Triglycerides were measured (mg•dl −1 ) by using the enzymatic colorimetric test kit (REF 20016) provided by the Randox ® Laboratories, BT29 4QY; Crumlin, County Antrim, UK on Biolab ® 320 at 500 nm wavelength. 17 Hormonal analysis: The Luteinizing hormone (IU•L −1 ), Follicle stimulating hormone (mIU•L −1 ), and Growth Hormone (mIU•L −1 ) were determined using the enzyme immunoassay kits (BioCheck, Inc., 323 Vintage Park Drive, Foster City, CA 94404) catalog numbers BC-1031, BC-1029, BC-1033 respectively. The readings were taken on ELISA Microplate reader URIT-660 ® , set at 450/630 nm wavelength.…”

Section: Animal Grouping and Handlingmentioning

confidence: 99%

Potential role of probiotic species in ameliorating oxidative stress, effect on liver profile and hormones in male albino rat model

et al. 2021

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Probiotics are living micro-organism preparations which can vigorously inhibit the probable pathogens colonization in the gut microbial ecology. Current experiment was designed to investigate the efficacy of imported probiotic species compared with the indigenous probiotics species on the oxidative stress, enzymes, and hormones in animal model. Thirty Albino rats were equally divided into three groups with 10 rats ( n = 10) in each group as Control (C), supplemented with imported probiotic species (IP), and supplemented with indigenous probiotics species (InP) for 21 days under controlled environment. The evaluation of treatments was done by testing the serum oxidative stress markers, liver enzymes (Aspartate transaminase and Alanine aminotransferase), lipid profile, and hormonal dynamics including Lutinizing hormone (LH), follicular stimulating hormone (FSH), and growth hormone (GH) in albino male rats. Results revealed that use of indigenous probiotic species significantly ( p < 0.05) reduces the oxidative stress and improves the antioxidant capacity; liver enzymes, total cholesterol, and LDL-Cholesterol were also reduced significantly ( p < 0.05) in InP as compared to IP group. Moreover, results of hormones including LH, FSH, and GH explored that indigenous probiotics have significant ( p < 0.05) potential to improve these hormones as compared to imported probiotics. Although, it could be concluded that InP have beneficial role in preventing the body from oxidative stress as well as in improving the blood parameters but comprehensive studies are required to investigate the detail gut ecology of the indigenous species which will definitely a strong support in preparing a more suitable local probiotic supplement.

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Shiitake Culinary-Medicinal Mushroom, Lentinus edodes (Agaricomycetes), Supplementation Alters Gut Microbiome and Corrects Dyslipidemia in Rats

Cited by 19 publications

References 0 publications

Dysbiosis of the Salivary Microbiome is Associated with Hypertension and Correlated with Metabolic Syndrome Biomarkers

Dysbiosis of the Salivary Microbiome is Associated with Hypertension and Correlated with Metabolic Syndrome Biomarkers

Profiling the Oral Microbiome and Plasma Biochemistry of Obese Hyperglycemic Subjects in Qatar

Potential role of probiotic species in ameliorating oxidative stress, effect on liver profile and hormones in male albino rat model

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