Shiga toxin and beta-lactamases genes in Escherichia coli phylotypes isolated from carcasses of broiler chickens slaughtered in Iran

Abstract

Two hundred and four Escherichia coli strains were isolated from external and visceral cavity surfaces of 102 slaughtered broiler carcasses. The isolates were screened to determine the phylogenetic background and presence of Shiga toxins (stx1, stx2), intimin (eae) and beta-lactamase (blaTEM, blaSHV) genes. Phylotyping results revealed that the E. coli isolates segregated in four phylogenetic groups A (56.86%), B1 (19.12%), B2 (4.90%) and D (19.12%). PCR assays revealed that 13 isolates (6.37%) from 12 carcasses were positive for eae (12 isolates) and/or stx2 (2) genes. The eae positive isolates belonged to phylogenetic groups A (A0, A1), B1, B2 (B22) and D (D2). Two stx2 positive and seven eae positive isolates were recovered from visceral cavity surface, whereas only 5 eae positive isolates were from the external surface of the carcasses. On the other hand, thirty one E. coli strains isolated from visceral cavity and external surface of 26 carcasses carried the blaTEM (27) and blaSHV (4) genes and belonged to different phylo-groups. This study suggests that broiler carcasses could be considered as an important source of EPEC and STEC pathotypes in southeast of Iran; as well as the examined antibiotic resistance genes, which were carried by some isolates and could be transferred to pathogens through the food chain.

Molecular analysis of multidrug resistance in Shiga toxin-producing Escherichia coli O157:H7 isolated from meat and dairy products

“…Our results are similar to those of a previous report from Canada, in which bla TEM , bla CMY , and bla SHV were found in 56%, 12%, and 4%, respectively, of ampicillin-resistant E. coli isolates recovered from a commercial beef processing plant (Aslam et al, 2009). However, the incidence of β-lactamase genes in isolates from our study is higher than in a recent report from Iran, in which 14.7% and 2.9% of STEC strains isolated from the external surfaces of chicken carcasses carried the bla TEM and bla SHV genes, respectively (Bagheri et al, 2014). In Japan, bla TEM-1 and bla CMY-2 were identified in 17.3% and 23.2% of E. coli isolates, respectively, from retail chicken meat (Ahmed et al, 2009).…”

Genetic diversity of Escherichia coli isolates of animal and environmental origins from an integrated poultry production chain

“…Group D was mainly found in the gut and respiratory tract (Table S1). The abundance of phylogenetic groups A and D in E. coli from broilers and the observation of B2 more represented in E. coli isolates from extraintestinal sites is in agreement with previous studies on commensal and ExPEC E. coli isolated from broilers, broiler carcasses and retail poultry products all over the world (Johnson et al, 2003(Johnson et al, , 2006Carlos et al, 2010; Values within a raw with different superscript letters are statistically different with P 0.05 Hussein et al, 2013;Aslam et al, 2014;Bagheri et al, 2014). Further studies on pathogenicity need to be performed on B2 isolates of the present study in order to verify whether these isolates are pathogenic similarly to extraintestinal B2 isolates described in humans (Bingen et al, 1998, Petersen et al, 2009Basu et al, 2013).…”

Comparative Analysis of Quinolone Resistance in Clinical Isolates ofKlebsiella pneumoniaeandEscherichia colifrom Chinese Children and Adults

“…Shortly after, antibiotic-resistant E. coli was detected in poultry products [ 24 ]. The situation of antibiotic-resistant bacteria from food chain sources has become a notable societal issue which should be worthy of attention [ 25 – 29 ]. Furthermore, pollution by antibiotic resistance genes from stock farming and antibiotics in manure and metabolites could increase the chances of resistance acquisition by human pathogens [ 30 ].…”