Molecular analysis of multidrug resistance in Shiga toxin-producing Escherichia coli O157:H7 isolated from meat and dairy products

Ahmed, Ashraf M.; Shimamoto, Tadashi

doi:10.1016/j.ijfoodmicro.2014.10.014

Cited by 73 publications

(59 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The potential source of cephalothin resistance in these environmental STEC O145 strains is unclear considering that this antibiotic is primarily for clinical use. High incidences of cephalothin resistance have been reported for E. coli strains isolated from both organic and conventional vegetables grown in South Korea (70), both farm and wild animals in India (71), dairy products from Iran (72), and various sources in Switzerland (73), implying the existence of widespread cephalothin resistance in environmental E. coli strains. Although the molecular basis of resistance in environmental STEC O145 strains is currently unclear, our data suggest that selective pressure for antimicrobial resistance may exist in the ecosystems from which the environmental strains were recovered.…”

Section: Resultsmentioning

confidence: 99%

An Environmental Shiga Toxin-Producing Escherichia coli O145 Clonal Population Exhibits High-Level Phenotypic Variation That Includes Virulence Traits

Carter

Quiñones

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

b Shiga toxin-producing Escherichia coli (STEC) serotype O145 is one of the major non-O157 serotypes associated with severe human disease. Here we examined the genetic diversity, population structure, virulence potential, and antimicrobial resistance profiles of environmental O145 strains recovered from a major produce production region in California. Multilocus sequence typing analyses revealed that sequence type 78 (ST-78), a common ST in clinical strains, was the predominant genotype among the environmental strains. Similarly, all California environmental strains belonged to H28, a common H serotype in clinical strains. Although most environmental strains carried an intact fliC gene, only one strain retained swimming motility. Diverse stx subtypes were identified, including stx 1a , stx 2a , stx 2c , and stx 2e . Although no correlation was detected between the stx genotype and Stx1 production, high Stx2 production was detected mainly in strains carrying stx 2a only and was correlated positively with the cytotoxicity of Shiga toxin. All environmental strains were capable of producing enterohemolysin, whereas only 10 strains were positive for anaerobic hemolytic activity. Multidrug resistance appeared to be common, as nearly half of the tested O145 strains displayed resistance to at least two different classes of antibiotics. The core virulence determinants of enterohemorrhagic E. coli were conserved in the environmental STEC O145 strains; however, there was large variation in the expression of virulence traits among the strains that were highly related genotypically, implying a trend of clonal divergence. Several cattle isolates exhibited key virulence traits comparable to those of the STEC O145 outbreak strains, emphasizing the emergence of hypervirulent strains in agricultural environments.

show abstract

Section: Resultsmentioning

confidence: 99%

An Environmental Shiga Toxin-Producing Escherichia coli O145 Clonal Population Exhibits High-Level Phenotypic Variation That Includes Virulence Traits

Carter

Quiñones

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…However, high level of MDR-bacteria isolated from various food animals have been documented in several African countries. Ahmed and Shimamoto (2015a,b) reported MDR-Shiga-toxin-producing E. coli O157:H7 (57.4%) and MDR- Shigella spp. (89%) from food products (meat and dairy products) in Egypt, while a maximum prevalence (100%) of MDR- E. coli has been identified in rectal samples of cattle in South Africa (Iweriebor et al, 2015).…”

Section: Current Status Of Antibiotic Resistance In the Food Chainmentioning

confidence: 99%

Antibiotic Resistance in the Food Chain: A Developing Country-Perspective

2016

View full text Add to dashboard Cite

Antibiotics are now “endangered species” facing extinction due to the worldwide emergence of antibiotic resistance (ABR). Food animals are considered as key reservoirs of antibiotic-resistant bacteria with the use of antibiotics in the food production industry having contributed to the actual global challenge of ABR. There are no geographic boundaries to impede the worldwide spread of ABR. If preventive and containment measures are not applied locally, nationally and regionally, the limited interventions in one country, continent and for instance, in the developing world, could compromise the efficacy and endanger ABR containment policies implemented in other parts of the world, the best-managed high-resource countries included. Multifaceted, comprehensive, and integrated measures complying with the One Health approach are imperative to ensure food safety and security, effectively combat infectious diseases, curb the emergence and spread of ABR, and preserve the efficacy of antibiotics for future generations. Countries should follow the World Health Organization, World Organization for Animal Health, and the Food and Agriculture Organization of the United Nations recommendations to implement national action plans encompassing human, (food) animal, and environmental sectors to improve policies, interventions and activities that address the prevention and containment of ABR from farm-to-fork. This review covers (i) the origin of antibiotic resistance, (ii) pathways by which bacteria spread to humans from farm-to-fork, (iii) differences in levels of antibiotic resistance between developed and developing countries, and (iv) prevention and containment measures of antibiotic resistance in the food chain.

show abstract

“…This classification displays resistances to other antibiotics may be encoded by other mechanisms or unwanted genes in this study. Studies carried out on E. coli producing shiga toxin in Egypt showed 3' conserved regions of integrons contains qnrB, qnrS, and floR genes encoding resistance to quinolones (Ahmed and Shimamoto, 2015). In this study, 80% (8/10) of diarrheagenic E. coli harboured class 1 integrons, with dfrA7 gene encoding trimethoprim resistance.…”

Section: Antimicrobial Resistance Of Diarrheagenic E Colimentioning

confidence: 60%

“…The studies on clinical, food and environmental DEC isolated from Nigeria, Egypt, India, and Kenya revealed that the presence of class 1, 2, and 3 integrons are the cause of the resistance of these pathogens to antibiotics. Class 1, and 2 integrons are connected to several resistance genes (cassettes) encoding antibiotic resistance such as dfr for trimethoprim resistance, aac and aad for aminoglycosides, sul for sulfonamides, tet for tetracycline's, cat, and cmlA1 for chloramphenicol, satA1 for streptothricin (Kiiru et al, 2013;Adelowo et al, 2014;Dureja et al, 2014;Ahmed and Shimamoto, 2015). Thus, this study aimed to examine the prevalence and mechanism of antibiotic resistance of diarrheagenic E. coli isolated from milk, pasteurized milk, curds, yogurts, and "déguè" (mixture of yogurt and millet lumps) consumed in Burkina Faso.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of enteropathogenic and enterotoxinogenic Escherichia coli from dairy products consumed in Burkina Faso

Bagré¹,

Sambe-Ba²,

Ibrahim³

et al. 2017

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Food-borne diseases represent a public major health problem, and drink-water, juice, meat, and milk products are usually involved. This study aimed to evaluate the antibiotic resistance of diarrheagenic E. coli isolated from dairy products consumed in Burkina Faso. Five hundred and twenty-two samples were gathered. Escherichia coli were isolated using Standard Microbiological Methods. A 16-plex polymerase chain reaction for virulence associated genes was applied. The standard disc diffusion methods were used to assess the susceptibility to 31 antibiotics. Classes 1, 2, 3 integrons were categorized using PCR. Results showed 1.92% (10/522) of milk products was contaminated by diarrheagenic E. coli. Enterotoxinogenic E. coli was found in 4.45% (4/89) of curds, 3.4% (3/88) of pasteurized milk, and 1.15% (1/87) of "déguè". Also, "déguè" was contaminated at 2.3% (2/87) by atypical enteropathogenic E. coli. Antibiogram susceptibility showed that pathogenic isolated resists mainly to tetracycline, amoxicillin, ticarcillin, nalidixic acid, sulfonamide, and trimethoprimsulfamethoxazole. Only the class 1 integrons was detected in 80% of diarrheagenic E. coli. Among this class 1 integrons, 4 strains contains a variable region, and the subsequent result showed a presence of dfrA7 gene coding for trimethoprim resistance. It appears in this study that dairy products are contaminated by enteropathogenic and enterotoxinogenic E. coli, which are resistant to antibiotics frequently used. This study therefore recommends the training of milk products transformers.

show abstract

Molecular analysis of multidrug resistance in Shiga toxin-producing Escherichia coli O157:H7 isolated from meat and dairy products

Cited by 73 publications

References 33 publications

An Environmental Shiga Toxin-Producing Escherichia coli O145 Clonal Population Exhibits High-Level Phenotypic Variation That Includes Virulence Traits

An Environmental Shiga Toxin-Producing Escherichia coli O145 Clonal Population Exhibits High-Level Phenotypic Variation That Includes Virulence Traits

Antibiotic Resistance in the Food Chain: A Developing Country-Perspective

Isolation and characterization of enteropathogenic and enterotoxinogenic Escherichia coli from dairy products consumed in Burkina Faso

Contact Info

Product

Resources

About