Shewanella spongiae sp. nov., isolated from a marine sponge

Abstract

A psychrophilic bacterium, designated strain HJ039 T , was isolated from a marine sponge collected in the East Sea of Korea (also known as the Sea of Japan). Cells were . Growth was observed between 5 and 26 6C (optimum 15 6C), at pH 5?0-8?5 (optimum pH 6?0-6?5) and in the presence of 0-6?0 % NaCl (optimum 2?0 %). The 16S rRNA gene sequence of strain HJ039 T showed high levels of similarity (93?7-95?4 %) with members of the genus Shewanella, especially with Shewanella gaetbuli TF-27 T (95?2 %), Shewanella decolorationis S12 T (94?9 %), Shewanella putrefaciens LMG 26268 T (94?6 %), Shewanella hafniensis P010 T (94?6 %), Shewanella algae ATCC 51192 T (94?5 %) and Shewanella kaireitica c931 T (94?5 %). However, phylogenetic analysis revealed that strain HJ039 T shared a phyletic line with S. algae and Shewanella amazonensis. The major respiratory quinone was Q-8. The DNA G+C content was 52?8 mol%. The major fatty acids were i-13 : 0 (8?5 %), 15 : 0 (4?2 %), i-15 : 0 (23?2 %), i-15 : 1 (7?9 %), 16 : 0 (8?7 %), 16 : 1v7 (21?0 %) and 17 : 1v8 (6?4 %). From this polyphasic taxonomic evidence, strain HJ039 T is considered to represent a novel species of the genus Shewanella, for which the name Shewanella spongiae sp. nov. is proposed. The type strain is HJ039 T (=KCCM 42304 T =JCM 13830 T ).

Gaetbulibacter aestuarii sp. nov., isolated from shallow coastal seawater, and emended description of the genus Gaetbulibacter

“…Isolation was achieved using the standard dilution-plating technique on marine agar (MA; Becton Dickinson) (Yang et al, 2006) and incubation at 25 u C for 7 days. Strain KYW382 T was routinely cultured on MA and preserved as a suspension in marine broth (MB; Becton Dickinson) containing 20 % (w/v) glycerol at -80 u C.…”

Pseudidiomarina aestuarii sp. nov., a marine bacterium isolated from shallow coastal seawater

“…Strain KYW314 T was isolated from seawater using the standard dilution-plating technique. Isolation was achieved using marine agar 2216 (MA; BD) (Yang et al, 2006) at 30 u C for 7 days. The isolate was routinely cultured on MA and maintained at -80 u C as a suspension in marine broth (MB; BD) containing glycerol (20 %, w/v).…”

“…Members of the genus Pseudidiomarina have been isolated from saline habitats with a wide range of salinities such as coastal and oceanic waters, seashore sands, coastal sediment and solar salterns (Jean et al, 2006(Jean et al, , 2009Kwon et al, 2006;Yoon et al, 2007;Hu & Li, 2007;Wu et al, 2009). During a survey of the diversity of heterotrophic marine bacteria, a bacterial strain, KYW314 T , was recovered from seawater samples collected from the shallow coastal region of Gwangyang Bay in the south of the Republic of Korea (34 u 519 N 128 u 479 E) in July 2008.…”

“…Negative for nitrate reduction, citrate utilization, production of H 2 S and indole and Table 1. Characteristics that serve to differentiate strain KYW314 T from the type strains of related Pseudidiomarina species Strains: 1, KYW314 T ; 2, P. homiensis KACC 11514 T (data in parentheses taken from Kwon et al, 2006); 3, P. taiwanensis BCRC 17465 T (Jean et al, 2006); 4, P. sediminum LMG 24046 T (Hu & Li, 2007); 5, P. salinarum CCUG 54359 T (Yoon et al, 2007). Data were obtained in this study unless indicated otherwise.…”

Shewanella haliotis sp. nov., isolated from the gut microflora of abalone, Haliotis discus hannai

“…Isolation was achieved using marine agar (MA; Difco) (Yang et al, 2006) at 30 u C for 7 days. The isolate was routinely cultured on MA and maintained as a glycerol suspension (20 %, w/v) at -80 u C.…”