Shell matrix protein genes derived from donor expressed in pearl sac of Akoya pearl oysters (Pinctada fucata) under pearl culture

Masaoka, Tetsuji; Samata, Tetsuro; Nogawa, Chihiro; Baba, Hiroko; Aoki, Hideo; Kotaki, Tomohiro; Nakagawa, Aoi; Sato, Masayuki; Fujiwara, Atushi; Kobayashi, Takanori

doi:10.1016/j.aquaculture.2012.12.019

Cited by 14 publications

(10 citation statements)

References 19 publications

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“…The increase or decrease in gene expression may be linked to the calcification rate [43], so that even the low expression of those proteins contributed to the gradual formation of the nacre lamellae with stair-like patterns. It has been reported that both N16 and N19 could inhibit the in vitro crystallization of calcite and the growth inhibition of undesired aragonite crystal faces is an effective way to modify the morphology of CaCO 3 crystals and orient nacre growth [22], [23], [37], [44], [45], while Pif80 can induce the nucleation of aragonite crystals and is directly involved in creating the layered arrangement of aragonite platelets in nacre [21]. Crystal nucleation and growth inhibition are both antagonistic mechanisms that are involved in nacre formation [46], [47].…”

Section: Discussionmentioning

confidence: 99%

Patterns of Expression in the Matrix Proteins Responsible for Nucleation and Growth of Aragonite Crystals in Flat Pearls of Pinctada fucata

Xiang

Zheng

et al. 2013

PLoS ONE

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The initial growth of the nacreous layer is crucial for comprehending the formation of nacreous aragonite. A flat pearl method in the presence of the inner-shell film was conducted to evaluate the role of matrix proteins in the initial stages of nacre biomineralization in vivo. We examined the crystals deposited on a substrate and the expression patterns of the matrix proteins in the mantle facing the substrate. In this study, the aragonite crystals nucleated on the surface at 5 days in the inner-shell film system. In the film-free system, the calcite crystals nucleated at 5 days, a new organic film covered the calcite, and the aragonite nucleated at 10 days. This meant that the nacre lamellae appeared in the inner-shell film system 5 days earlier than that in the film-free system, timing that was consistent with the maximum level of matrix proteins during the first 20 days. In addition, matrix proteins (Nacrein, MSI60, N19, N16 and Pif80) had similar expression patterns in controlling the sequential morphologies of the nacre growth in the inner-film system, while these proteins in the film-free system also had similar patterns of expression. These results suggest that matrix proteins regulate aragonite nucleation and growth with the inner-shell film in vivo.

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Section: Discussionmentioning

confidence: 99%

Patterns of Expression in the Matrix Proteins Responsible for Nucleation and Growth of Aragonite Crystals in Flat Pearls of Pinctada fucata

Xiang

Zheng

et al. 2013

PLoS ONE

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“…The epithelial cells from the outer surface of the mantle tissue (which lines the inner surface of the shell) are capable of synthesising different calcium carbonate polymorphs (Awaji & Machii, ; Watabe, ; Wilbur, ), which cover the nucleus, as observed by electron microscopy (Zhang & Xu, ). The genome of the donor oyster is still present in the pearl sac of the recipient oyster at the end of pearl formation and its influence on the pearl biomineralisation process still active (Arnaud‐Haond et al., ; Masaoka et al., ). Correlation of cultured pearl nacre weight and thickness with gene expression levels assessed by real‐time PCR from a representative panel of matrix protein genes revealed that pif177 and pearlin expression in the pearl sac of P. margaritifera were correlated with both nacre weight and thickness in Arutua, but not significantly in Apataki.…”

Section: Discussionmentioning

confidence: 99%

Donor effect on cultured pearl nacre development and shell matrix gene expression inPinctada margaritiferareared in different field sites

Broustal

Koua

et al. 2018

Aquac Res

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Understanding the respective roles played by donor and recipient pearl oysters in pearl quality determination in relation to the environment is a challenge for the pearl industry. In most Pinctada species, pearl size is mainly related to recipient oyster growth performance but also relies to some extent on the biomineralisation activity of the pearl sac, a tissue that originates from the donor oyster mantle. We examined donor effect on pearl size in response to culture in the lagoons on Arutua and Apataki atolls. Overall, nacre weight and thickness were greater in Arutua than in Apataki, but sensitivity to the environment differed between donors. Some donors were associated with significantly heavier and thicker nacre in Arutua (I group), while others had similar results at the two sites (NI group). On average, up to 20% of the pearl size could be attributed to the donor but, in group I, donor effect was responsible for up to 36% of nacre weight determination. Additionally, a real‐time PCR expression study of eight matrix protein genes related to biomineralisation in the pearl sac showed that MSI60, pearlin and pif177 were significantly and positively correlated with nacre weight and thickness, with the latter two genes explaining the larger pearl size observed in Arutua. Donor oysters in P. margaritifera therefore play a key role in pearl size improvement, related to the role of the shell matrix protein genes. Understanding such contributions could help in the design of genetic selection plans for specific and adapted donor oyster lines.

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“…Pearl culture is an important industry in China and many other countries in the world (Masaoka et al, 2013). Pearl culture contains four steps: 1) pre-operative conditioning, 2) nucleus implantation, 3) post-operative care, and 4) culturing and harvest (Wada, 1999;Lucas and Southgate, 2008).…”

Section: Introductionmentioning

confidence: 99%

Highly expressed EGFR in pearl sac may facilitate the pearl formation in the pearl oyster, Pinctada fucata

Wang

Fan

Huang

et al. 2015

Gene

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Shell matrix protein genes derived from donor expressed in pearl sac of Akoya pearl oysters (Pinctada fucata) under pearl culture

Cited by 14 publications

References 19 publications

Patterns of Expression in the Matrix Proteins Responsible for Nucleation and Growth of Aragonite Crystals in Flat Pearls of Pinctada fucata

Patterns of Expression in the Matrix Proteins Responsible for Nucleation and Growth of Aragonite Crystals in Flat Pearls of Pinctada fucata

Donor effect on cultured pearl nacre development and shell matrix gene expression inPinctada margaritiferareared in different field sites

Highly expressed EGFR in pearl sac may facilitate the pearl formation in the pearl oyster, Pinctada fucata

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