Sheep gene mapping: additional DNA markers included (CASB, CASK, LALBA, IGF‐1 and AMH).

Abstract

Summary. DNA extracted from 25 hamster‐sheep hybrid cell lines was subjected, after Southern blotting, to hybridization with CASB, CASK, LALBA, IGF‐1 and AMH cDNA probes. CASB and CASK segregated together and IGF‐1 and LALBA were found syntenic with the LDHB‐PEPB‐TPI‐GAPD‐SHMT‐KRTB group. No other synteny was observed with any of the previously described groups using the same hybrid cell panel. Gene nomenclature: ACO 1: aconitase 1 (soluble); ADA: adenosine deaminase; AMH: antiMüllerian hormone; ARA 1: murine sarcoma 3611 viral (v‐raf) oncogene homologue 1; CASB: ß‐casein; CASK: K‐casein; ENO 1: enolase 1 (alpha); GAPD: glucose‐6‐phosphate dehydrogenase; GALA (or GLA): galactosidase (alpha); GAPD; glyceraldehyde‐3‐phosphate dehydrogenase; GPI: glucose phosphate isomerase; GSR: glutathione reductase; HBG: haemoglobin gamma; HPRT: hypoxanthine phosphoribosyl transferase; IDH 1: isocitrate dehydrogenase 1 (soluble); IGF‐1: insulin growth factor 1; ITPA: inosine triphosphatase; KRTA: keratin (acid); KRTB: keratin (basic); LALBA: aT‐lactalbumin; LDHA: lactate dehydrogenase A; LDHB: lactate dehydrogenase B; MDH 2: malate dehydrogenase NAD (soluble); ME 1: malic enzyme (soluble); MPI: mannose phosphate isomerase; NP: nucleoside phosphorylase; OLA: ovine leucocyte antigen: OTC: ornitine carbamoyltransferase; PAIS: phosphoribosyl amino imidazole synthetase; PEPA, PEPB, PEPC: peptidase A, B, C; PGD: phospho gluconate dehydrogenase; PGK: phosphoglycerate kinase; PGM 3: phospho glucomutase 3; PKM 2: pyruvate kinase (muscle); PLP: proteolipid protein; PRGS: phosphoribosyl glycinamide synthetase; RCP: red cone pigment; SHMT: serine hydroxymethyl transferase; SOD 1: superoxide dismutase 1 (soluble); SYN 1: synapsin 1; TPI1: triose phosphate isomerase 1.

Cow management modulates gene expression in the mammary gland, a possible epigenetics role

“…The lactoglobulin gene (LGB), on the other hand, is encoded on chromosome 11 in the three species. The third main protein is lactalbumin, codified by the gene LA assigned to chromosome 3 in sheep (Ghali et al, 1991), while in goats, LA has been mapped on chromosome 6, and in cows on chromosome 5 (Hayes and Petit, 1993). Genes that codified for the major milk proteins (caseins, lactoglobulin and lactalbumin) genes are characterized by a high rate of polymorphism and are expressed in a co-dominant fashion.…”